Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_113 and Pf6N2E2_114 are separated by 104 nucleotides Pf6N2E2_114 and Pf6N2E2_115 overlap by 4 nucleotides
Pf6N2E2_113 - FIG00963512: hypothetical protein, at 158,312 to 160,225
_113
Pf6N2E2_114 - Polysaccharide biosynthesis protein, at 160,330 to 161,634
_114
Pf6N2E2_115 - FIG00958408: hypothetical protein, at 161,631 to 162,635
_115
Position (kb) 160
161
162 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.507 kb on + strand, within Pf6N2E2_113 at 159.521 kb on - strand, within Pf6N2E2_113 at 159.735 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.955 kb on - strand, within Pf6N2E2_113 at 160.093 kb on - strand at 160.150 kb on - strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.297 kb on + strand at 160.387 kb on - strand at 160.427 kb on + strand at 160.428 kb on - strand at 160.460 kb on + strand at 160.461 kb on - strand, within Pf6N2E2_114 at 160.461 kb on - strand, within Pf6N2E2_114 at 161.390 kb on - strand, within Pf6N2E2_114 at 161.618 kb on + strand at 161.741 kb on - strand, within Pf6N2E2_115 at 161.828 kb on - strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 162.085 kb on + strand, within Pf6N2E2_115 at 162.086 kb on - strand, within Pf6N2E2_115 at 162.086 kb on - strand at 162.278 kb on + strand, within Pf6N2E2_115
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 159,441 + Pf6N2E2_113 0.59 -0.6 159,441 + Pf6N2E2_113 0.59 +0.3 159,441 + Pf6N2E2_113 0.59 +0.1 159,444 + Pf6N2E2_113 0.59 +1.1 159,444 + Pf6N2E2_113 0.59 -0.8 159,444 + Pf6N2E2_113 0.59 -0.3 159,444 + Pf6N2E2_113 0.59 +0.2 159,445 - Pf6N2E2_113 0.59 +0.0 159,445 - Pf6N2E2_113 0.59 -0.4 159,445 - Pf6N2E2_113 0.59 -0.1 159,507 + Pf6N2E2_113 0.62 -0.7 159,521 - Pf6N2E2_113 0.63 +0.1 159,735 + Pf6N2E2_113 0.74 +0.5 159,864 + Pf6N2E2_113 0.81 -0.3 159,864 + Pf6N2E2_113 0.81 +0.4 159,865 - Pf6N2E2_113 0.81 -0.5 159,865 - Pf6N2E2_113 0.81 +0.5 159,865 - Pf6N2E2_113 0.81 +0.1 159,865 - Pf6N2E2_113 0.81 -0.2 159,955 - Pf6N2E2_113 0.86 -0.5 160,093 - -0.5 160,150 - +0.2 160,173 + -0.1 160,173 + +0.1 160,173 + -0.8 160,297 + -1.3 160,387 - -0.3 160,427 + +1.8 160,428 - -0.4 160,460 + +1.9 160,461 - Pf6N2E2_114 0.10 +0.2 160,461 - Pf6N2E2_114 0.10 -1.7 161,390 - Pf6N2E2_114 0.81 -1.3 161,618 + +0.6 161,741 - Pf6N2E2_115 0.11 +0.4 161,828 - Pf6N2E2_115 0.20 +0.4 161,833 + Pf6N2E2_115 0.20 +0.1 161,833 + Pf6N2E2_115 0.20 +1.1 161,833 + Pf6N2E2_115 0.20 -0.1 161,833 + Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 +0.2 161,834 - Pf6N2E2_115 0.20 +0.1 161,834 - Pf6N2E2_115 0.20 +1.6 161,834 - Pf6N2E2_115 0.20 +0.1 161,834 - Pf6N2E2_115 0.20 +1.1 161,834 - Pf6N2E2_115 0.20 -1.5 161,834 - Pf6N2E2_115 0.20 -0.1 161,834 - Pf6N2E2_115 0.20 -0.2 161,834 - Pf6N2E2_115 0.20 +0.6 162,085 + Pf6N2E2_115 0.45 +0.7 162,086 - Pf6N2E2_115 0.45 -0.6 162,086 - -2.1 162,278 + Pf6N2E2_115 0.64 -1.1
Or see this region's nucleotide sequence