Experiment: formate McAA pantothenate dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06840 and MMJJ_RS06845 are separated by 13 nucleotides MMJJ_RS06845 and MMJJ_RS06850 are separated by 22 nucleotides MMJJ_RS06850 and MMJJ_RS06855 are separated by 15 nucleotides MMJJ_RS06855 and MMJJ_RS06860 are separated by 4 nucleotides MMJJ_RS06860 and MMJJ_RS06865 are separated by 8 nucleotides
MMJJ_RS06840: MMJJ_RS06840 - nickel-dependent hydrogenase large subunit, at 1,262,588 to 1,263,715
_RS06840
MMJJ_RS06845: MMJJ_RS06845 - NADH-quinone oxidoreductase subunit B family protein, at 1,263,729 to 1,264,199
_RS06845
MMJJ_RS06850: MMJJ_RS06850 - DUF1959 domain-containing protein, at 1,264,222 to 1,264,632
_RS06850
MMJJ_RS06855: MMJJ_RS06855 - DUF2104 domain-containing protein, at 1,264,648 to 1,264,947
_RS06855
MMJJ_RS06860: MMJJ_RS06860 - hypothetical protein, at 1,264,952 to 1,265,203
_RS06860
MMJJ_RS06865: MMJJ_RS06865 - NADH-quinone oxidoreductase subunit H, at 1,265,212 to 1,266,072
_RS06865
Position (kb)
1264
1265 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1263.271 kb on - strand, within MMJJ_RS06840 at 1263.285 kb on + strand, within MMJJ_RS06840 at 1263.299 kb on + strand, within MMJJ_RS06840 at 1263.430 kb on - strand, within MMJJ_RS06840 at 1263.435 kb on + strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.516 kb on - strand, within MMJJ_RS06840 at 1263.553 kb on - strand at 1263.565 kb on + strand, within MMJJ_RS06840 at 1263.633 kb on + strand at 1263.715 kb on - strand at 1263.718 kb on - strand at 1263.902 kb on - strand, within MMJJ_RS06845 at 1263.915 kb on + strand, within MMJJ_RS06845 at 1264.061 kb on - strand at 1264.117 kb on + strand, within MMJJ_RS06845 at 1264.223 kb on - strand at 1264.224 kb on + strand at 1264.246 kb on + strand at 1264.252 kb on + strand at 1264.252 kb on + strand at 1264.381 kb on - strand, within MMJJ_RS06850 at 1264.627 kb on - strand at 1264.649 kb on - strand at 1264.650 kb on + strand at 1264.666 kb on - strand at 1264.670 kb on + strand at 1264.683 kb on - strand, within MMJJ_RS06855 at 1264.684 kb on + strand at 1264.693 kb on - strand, within MMJJ_RS06855 at 1264.760 kb on + strand, within MMJJ_RS06855 at 1264.924 kb on - strand at 1264.927 kb on - strand at 1264.988 kb on + strand, within MMJJ_RS06860 at 1264.997 kb on - strand, within MMJJ_RS06860 at 1265.026 kb on - strand, within MMJJ_RS06860 at 1265.066 kb on - strand, within MMJJ_RS06860 at 1265.137 kb on - strand, within MMJJ_RS06860 at 1265.140 kb on - strand, within MMJJ_RS06860 at 1265.140 kb on - strand, within MMJJ_RS06860 at 1265.140 kb on - strand, within MMJJ_RS06860 at 1265.145 kb on + strand at 1265.193 kb on - strand at 1265.194 kb on + strand at 1265.228 kb on - strand at 1265.316 kb on - strand, within MMJJ_RS06865 at 1265.375 kb on + strand, within MMJJ_RS06865 at 1265.499 kb on - strand, within MMJJ_RS06865
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA pantothenate dropout remove 1,263,271 - MMJJ_RS06840 0.61 -0.6 1,263,285 + MMJJ_RS06840 0.62 -2.4 1,263,299 + MMJJ_RS06840 0.63 -2.0 1,263,430 - MMJJ_RS06840 0.75 -2.1 1,263,435 + MMJJ_RS06840 0.75 -2.1 1,263,516 - MMJJ_RS06840 0.82 -2.4 1,263,516 - MMJJ_RS06840 0.82 -2.0 1,263,553 - -1.0 1,263,565 + MMJJ_RS06840 0.87 +0.8 1,263,633 + -1.6 1,263,715 - -2.8 1,263,718 - -1.1 1,263,902 - MMJJ_RS06845 0.37 -1.3 1,263,915 + MMJJ_RS06845 0.39 -1.3 1,264,061 - -0.6 1,264,117 + MMJJ_RS06845 0.82 -2.0 1,264,223 - -3.0 1,264,224 + +0.0 1,264,246 + -2.1 1,264,252 + -3.1 1,264,252 + -1.8 1,264,381 - MMJJ_RS06850 0.39 -2.2 1,264,627 - -2.4 1,264,649 - -1.7 1,264,650 + -4.1 1,264,666 - -2.0 1,264,670 + -1.0 1,264,683 - MMJJ_RS06855 0.12 -3.3 1,264,684 + +0.4 1,264,693 - MMJJ_RS06855 0.15 -2.0 1,264,760 + MMJJ_RS06855 0.37 -1.0 1,264,924 - -1.8 1,264,927 - -2.2 1,264,988 + MMJJ_RS06860 0.14 -3.4 1,264,997 - MMJJ_RS06860 0.18 -4.0 1,265,026 - MMJJ_RS06860 0.29 -3.4 1,265,066 - MMJJ_RS06860 0.45 -3.8 1,265,137 - MMJJ_RS06860 0.73 -2.5 1,265,140 - MMJJ_RS06860 0.75 -3.9 1,265,140 - MMJJ_RS06860 0.75 -3.1 1,265,140 - MMJJ_RS06860 0.75 -2.9 1,265,145 + -0.6 1,265,193 - -3.7 1,265,194 + -2.7 1,265,228 - -0.6 1,265,316 - MMJJ_RS06865 0.12 -2.8 1,265,375 + MMJJ_RS06865 0.19 -1.6 1,265,499 - MMJJ_RS06865 0.33 -3.3
Or see this region's nucleotide sequence