Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05003

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05001 and H281DRAFT_05002 overlap by 16 nucleotidesH281DRAFT_05002 and H281DRAFT_05003 overlap by 4 nucleotidesH281DRAFT_05003 and H281DRAFT_05004 overlap by 4 nucleotidesH281DRAFT_05004 and H281DRAFT_05005 are separated by 54 nucleotides H281DRAFT_05001: H281DRAFT_05001 - type III secretion protein L, at 187,273 to 188,052 _05001 H281DRAFT_05002: H281DRAFT_05002 - type III secretion protein (HrpB4), at 188,037 to 188,852 _05002 H281DRAFT_05003: H281DRAFT_05003 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 188,849 to 189,670 _05003 H281DRAFT_05004: H281DRAFT_05004 - type III secretion inner rod protein HrpB2, at 189,667 to 190,059 _05004 H281DRAFT_05005: H281DRAFT_05005 - type III secretion protein HrpB1, at 190,114 to 190,731 _05005 Position (kb) 188 189 190Strain fitness (log2 ratio) -2 -1 0 1at 188.249 kb on + strand, within H281DRAFT_05002at 188.689 kb on + strand, within H281DRAFT_05002at 188.690 kb on - strand, within H281DRAFT_05002at 188.694 kb on - strand, within H281DRAFT_05002at 188.833 kb on + strandat 188.834 kb on - strandat 189.103 kb on + strand, within H281DRAFT_05003at 189.104 kb on - strand, within H281DRAFT_05003at 190.585 kb on - strand, within H281DRAFT_05005

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
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188,249 + H281DRAFT_05002 0.26 -0.1
188,689 + H281DRAFT_05002 0.80 -0.4
188,690 - H281DRAFT_05002 0.80 -2.7
188,694 - H281DRAFT_05002 0.81 +0.1
188,833 + +0.3
188,834 - +0.5
189,103 + H281DRAFT_05003 0.31 +0.2
189,104 - H281DRAFT_05003 0.31 -1.1
190,585 - H281DRAFT_05005 0.76 -0.8

Or see this region's nucleotide sequence