Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01320

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01318 and H281DRAFT_01319 are separated by 54 nucleotidesH281DRAFT_01319 and H281DRAFT_01320 are separated by 93 nucleotidesH281DRAFT_01320 and H281DRAFT_01321 are separated by 103 nucleotides H281DRAFT_01318: H281DRAFT_01318 - hypothetical protein, at 159,218 to 159,802 _01318 H281DRAFT_01319: H281DRAFT_01319 - Uncharacterized protein, UPF0264 family, at 159,857 to 160,600 _01319 H281DRAFT_01320: H281DRAFT_01320 - Uncharacterized archaeal kinase related to aspartokinases, uridylate kinases, at 160,694 to 161,284 _01320 H281DRAFT_01321: H281DRAFT_01321 - probable H4MPT-linked C1 transfer pathway protein, at 161,388 to 162,512 _01321 Position (kb) 160 161 162Strain fitness (log2 ratio) -2 -1 0 1 2 3at 159.811 kb on - strandat 160.118 kb on + strand, within H281DRAFT_01319at 160.598 kb on + strandat 160.599 kb on - strandat 160.634 kb on - strandat 160.747 kb on + strandat 160.747 kb on + strandat 160.767 kb on + strand, within H281DRAFT_01320at 161.819 kb on - strand, within H281DRAFT_01321at 162.184 kb on + strand, within H281DRAFT_01321

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
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159,811 - +0.1
160,118 + H281DRAFT_01319 0.35 +0.8
160,598 + -0.2
160,599 - +0.9
160,634 - -2.7
160,747 + -1.4
160,747 + +2.8
160,767 + H281DRAFT_01320 0.12 -0.6
161,819 - H281DRAFT_01321 0.38 +1.8
162,184 + H281DRAFT_01321 0.71 +0.2

Or see this region's nucleotide sequence