Experiment: nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS18695 and ABZR86_RS18700 are separated by 364 nucleotides ABZR86_RS18700 and ABZR86_RS18705 are separated by 157 nucleotides ABZR86_RS18705 and ABZR86_RS18710 are separated by 195 nucleotides
ABZR86_RS18695: ABZR86_RS18695 - hypothetical protein, at 164,049 to 164,651
_RS18695
ABZR86_RS18700: ABZR86_RS18700 - YdcH family protein, at 165,016 to 165,252
_RS18700
ABZR86_RS18705: ABZR86_RS18705 - pyridoxal-phosphate dependent enzyme, at 165,410 to 166,780
_RS18705
ABZR86_RS18710: ABZR86_RS18710 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 166,976 to 168,805
_RS18710
Position (kb)
165
166
167 Strain fitness (log2 ratio)
-2
-1
0
1 at 164.440 kb on - strand, within ABZR86_RS18695 at 164.440 kb on - strand, within ABZR86_RS18695 at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.649 kb on + strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.650 kb on - strand at 164.829 kb on + strand at 164.830 kb on - strand at 164.918 kb on + strand at 165.085 kb on + strand, within ABZR86_RS18700 at 165.085 kb on + strand, within ABZR86_RS18700 at 165.085 kb on + strand, within ABZR86_RS18700 at 165.085 kb on + strand, within ABZR86_RS18700 at 165.085 kb on + strand, within ABZR86_RS18700 at 165.085 kb on + strand, within ABZR86_RS18700 at 165.086 kb on - strand, within ABZR86_RS18700 at 165.086 kb on - strand, within ABZR86_RS18700 at 165.250 kb on + strand at 165.251 kb on - strand at 165.251 kb on - strand at 165.251 kb on - strand at 165.251 kb on - strand at 165.251 kb on - strand at 165.349 kb on + strand at 165.349 kb on + strand at 165.349 kb on + strand at 165.789 kb on - strand, within ABZR86_RS18705 at 166.289 kb on - strand, within ABZR86_RS18705 at 166.328 kb on + strand, within ABZR86_RS18705 at 166.344 kb on - strand, within ABZR86_RS18705 at 166.762 kb on - strand at 166.762 kb on - strand at 166.768 kb on + strand at 166.778 kb on + strand at 166.779 kb on - strand at 166.779 kb on - strand at 166.779 kb on - strand at 166.779 kb on - strand at 166.792 kb on + strand at 166.792 kb on + strand at 166.792 kb on + strand at 166.793 kb on - strand at 166.793 kb on - strand at 166.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 150 mM remove 164,440 - ABZR86_RS18695 0.65 -0.9 164,440 - ABZR86_RS18695 0.65 +0.3 164,649 + -0.1 164,649 + -0.6 164,649 + -0.4 164,649 + -0.9 164,649 + +0.3 164,649 + -0.3 164,649 + +0.3 164,649 + -0.7 164,650 - +0.3 164,650 - -0.5 164,650 - -0.3 164,650 - -0.3 164,650 - -1.1 164,650 - -0.4 164,650 - -0.3 164,650 - -0.2 164,650 - +0.1 164,650 - -0.1 164,829 + -0.2 164,830 - -0.3 164,918 + +0.1 165,085 + ABZR86_RS18700 0.29 +1.2 165,085 + ABZR86_RS18700 0.29 +0.5 165,085 + ABZR86_RS18700 0.29 +0.0 165,085 + ABZR86_RS18700 0.29 -0.4 165,085 + ABZR86_RS18700 0.29 -0.4 165,085 + ABZR86_RS18700 0.29 -0.5 165,086 - ABZR86_RS18700 0.30 +0.3 165,086 - ABZR86_RS18700 0.30 -0.0 165,250 + -0.2 165,251 - +0.4 165,251 - +0.1 165,251 - -0.3 165,251 - +1.2 165,251 - -0.2 165,349 + +0.0 165,349 + -0.2 165,349 + +1.5 165,789 - ABZR86_RS18705 0.28 -1.3 166,289 - ABZR86_RS18705 0.64 -0.1 166,328 + ABZR86_RS18705 0.67 -1.8 166,344 - ABZR86_RS18705 0.68 +0.5 166,762 - +0.9 166,762 - +0.5 166,768 + -0.9 166,778 + -0.6 166,779 - -0.3 166,779 - -0.1 166,779 - +0.2 166,779 - +0.6 166,792 + +0.1 166,792 + +0.4 166,792 + -0.1 166,793 - +0.5 166,793 - +0.6 166,793 - +0.2
Or see this region's nucleotide sequence