Experiment: nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS15450 and ABZR86_RS15455 overlap by 8 nucleotides ABZR86_RS15455 and ABZR86_RS15460 are separated by 378 nucleotides ABZR86_RS15460 and ABZR86_RS15465 are separated by 123 nucleotides ABZR86_RS15465 and ABZR86_RS15470 are separated by 137 nucleotides
ABZR86_RS15450: ABZR86_RS15450 - FecR domain-containing protein, at 3,477,422 to 3,478,465
_RS15450
ABZR86_RS15455: ABZR86_RS15455 - RNA polymerase sigma factor, at 3,478,458 to 3,478,973
_RS15455
ABZR86_RS15460: ABZR86_RS15460 - type I glutamate--ammonia ligase, at 3,479,352 to 3,480,761
_RS15460
ABZR86_RS15465: ABZR86_RS15465 - glutamine amidotransferase, at 3,480,885 to 3,481,580
_RS15465
ABZR86_RS15470: ABZR86_RS15470 - hypothetical protein, at 3,481,718 to 3,482,341
_RS15470
Position (kb)
3479
3480
3481 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3478.404 kb on + strand at 3478.405 kb on - strand at 3478.466 kb on - strand at 3478.510 kb on + strand, within ABZR86_RS15455 at 3478.510 kb on + strand, within ABZR86_RS15455 at 3478.511 kb on - strand, within ABZR86_RS15455 at 3478.580 kb on - strand, within ABZR86_RS15455 at 3478.792 kb on + strand, within ABZR86_RS15455 at 3478.852 kb on + strand, within ABZR86_RS15455 at 3478.852 kb on + strand, within ABZR86_RS15455 at 3478.853 kb on - strand, within ABZR86_RS15455 at 3478.853 kb on - strand, within ABZR86_RS15455 at 3478.853 kb on - strand, within ABZR86_RS15455 at 3478.853 kb on - strand, within ABZR86_RS15455 at 3478.896 kb on - strand, within ABZR86_RS15455 at 3479.010 kb on + strand at 3479.011 kb on - strand at 3479.011 kb on - strand at 3479.011 kb on - strand at 3479.017 kb on + strand at 3479.018 kb on - strand at 3479.018 kb on - strand at 3479.042 kb on + strand at 3479.043 kb on - strand at 3479.043 kb on - strand at 3479.043 kb on - strand at 3479.120 kb on + strand at 3479.120 kb on + strand at 3479.120 kb on + strand at 3479.120 kb on + strand at 3479.144 kb on + strand at 3479.144 kb on + strand at 3479.144 kb on + strand at 3479.145 kb on - strand at 3479.145 kb on - strand at 3479.145 kb on - strand at 3479.145 kb on - strand at 3479.653 kb on - strand, within ABZR86_RS15460 at 3480.214 kb on - strand, within ABZR86_RS15460 at 3480.309 kb on + strand, within ABZR86_RS15460 at 3480.456 kb on + strand, within ABZR86_RS15460 at 3480.678 kb on + strand at 3480.748 kb on - strand at 3480.748 kb on - strand at 3480.750 kb on + strand at 3480.849 kb on - strand at 3480.849 kb on - strand at 3480.849 kb on - strand at 3481.578 kb on + strand at 3481.615 kb on + strand at 3481.615 kb on + strand at 3481.664 kb on + strand at 3481.679 kb on - strand at 3481.711 kb on + strand at 3481.711 kb on + strand at 3481.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 150 mM remove 3,478,404 + -0.0 3,478,405 - +0.0 3,478,466 - +0.0 3,478,510 + ABZR86_RS15455 0.10 +0.2 3,478,510 + ABZR86_RS15455 0.10 -0.4 3,478,511 - ABZR86_RS15455 0.10 +0.4 3,478,580 - ABZR86_RS15455 0.24 +1.0 3,478,792 + ABZR86_RS15455 0.65 +0.6 3,478,852 + ABZR86_RS15455 0.76 +0.1 3,478,852 + ABZR86_RS15455 0.76 +0.2 3,478,853 - ABZR86_RS15455 0.77 +0.3 3,478,853 - ABZR86_RS15455 0.77 +0.1 3,478,853 - ABZR86_RS15455 0.77 -0.2 3,478,853 - ABZR86_RS15455 0.77 -0.6 3,478,896 - ABZR86_RS15455 0.85 +1.2 3,479,010 + -0.4 3,479,011 - +0.3 3,479,011 - -0.2 3,479,011 - +0.0 3,479,017 + -0.3 3,479,018 - +0.0 3,479,018 - -0.4 3,479,042 + -0.3 3,479,043 - -0.2 3,479,043 - +0.0 3,479,043 - -0.2 3,479,120 + +0.1 3,479,120 + -0.1 3,479,120 + +0.0 3,479,120 + -0.8 3,479,144 + +0.0 3,479,144 + -0.1 3,479,144 + -0.4 3,479,145 - +0.1 3,479,145 - +0.8 3,479,145 - -1.0 3,479,145 - -0.1 3,479,653 - ABZR86_RS15460 0.21 -0.3 3,480,214 - ABZR86_RS15460 0.61 -3.0 3,480,309 + ABZR86_RS15460 0.68 -1.9 3,480,456 + ABZR86_RS15460 0.78 -0.7 3,480,678 + +0.5 3,480,748 - -1.3 3,480,748 - +0.3 3,480,750 + +0.2 3,480,849 - +0.1 3,480,849 - -0.1 3,480,849 - -0.1 3,481,578 + +0.9 3,481,615 + -0.3 3,481,615 + +0.0 3,481,664 + +0.2 3,481,679 - +0.3 3,481,711 + +0.4 3,481,711 + +1.1 3,481,712 - +0.2
Or see this region's nucleotide sequence