Experiment: nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS13925 and ABZR86_RS13930 overlap by 4 nucleotides ABZR86_RS13930 and ABZR86_RS13935 are separated by 93 nucleotides ABZR86_RS13935 and ABZR86_RS13940 are separated by 217 nucleotides ABZR86_RS13940 and ABZR86_RS13945 are separated by 165 nucleotides
ABZR86_RS13925: ABZR86_RS13925 - glycosyltransferase family 39 protein, at 3,105,330 to 3,107,036
_RS13925
ABZR86_RS13930: ABZR86_RS13930 - hypothetical protein, at 3,107,033 to 3,107,362
_RS13930
ABZR86_RS13935: ABZR86_RS13935 - lipid A biosynthesis acyltransferase, at 3,107,456 to 3,108,364
_RS13935
ABZR86_RS13940: ABZR86_RS13940 - D-aminoacyl-tRNA deacylase, at 3,108,582 to 3,109,022
_RS13940
ABZR86_RS13945: ABZR86_RS13945 - RNA polymerase sigma factor RpoD, at 3,109,188 to 3,111,047
_RS13945
Position (kb)
3107
3108
3109 Strain fitness (log2 ratio)
-2
-1
0
1 at 3107.361 kb on - strand at 3107.361 kb on - strand at 3107.361 kb on - strand at 3107.361 kb on - strand at 3107.445 kb on - strand at 3107.490 kb on + strand at 3107.526 kb on + strand at 3107.526 kb on + strand at 3107.526 kb on + strand at 3107.526 kb on + strand at 3107.526 kb on + strand at 3107.527 kb on - strand at 3107.527 kb on - strand at 3107.538 kb on + strand at 3107.538 kb on + strand at 3107.539 kb on - strand at 3107.539 kb on - strand at 3107.539 kb on - strand at 3107.539 kb on - strand at 3107.547 kb on + strand, within ABZR86_RS13935 at 3107.547 kb on + strand, within ABZR86_RS13935 at 3107.548 kb on - strand, within ABZR86_RS13935 at 3107.982 kb on + strand, within ABZR86_RS13935 at 3107.982 kb on + strand, within ABZR86_RS13935 at 3107.983 kb on - strand, within ABZR86_RS13935 at 3107.983 kb on - strand, within ABZR86_RS13935 at 3108.287 kb on + strand at 3108.288 kb on - strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.339 kb on + strand at 3108.340 kb on - strand at 3108.340 kb on - strand at 3108.340 kb on - strand at 3108.340 kb on - strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.348 kb on + strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.349 kb on - strand at 3108.449 kb on - strand at 3108.449 kb on - strand at 3108.464 kb on + strand at 3108.464 kb on + strand at 3108.465 kb on - strand at 3108.465 kb on - strand at 3108.465 kb on - strand at 3108.465 kb on - strand at 3108.496 kb on + strand at 3108.496 kb on + strand at 3108.497 kb on - strand at 3108.527 kb on + strand at 3108.543 kb on + strand at 3108.543 kb on + strand at 3108.543 kb on + strand at 3108.544 kb on - strand at 3108.578 kb on - strand at 3108.965 kb on + strand, within ABZR86_RS13940 at 3108.965 kb on + strand, within ABZR86_RS13940 at 3108.965 kb on + strand, within ABZR86_RS13940 at 3108.966 kb on - strand, within ABZR86_RS13940 at 3108.966 kb on - strand, within ABZR86_RS13940 at 3108.966 kb on - strand, within ABZR86_RS13940 at 3109.069 kb on - strand at 3109.098 kb on - strand at 3109.106 kb on + strand at 3109.106 kb on + strand at 3109.107 kb on - strand at 3109.112 kb on + strand at 3109.113 kb on - strand at 3109.153 kb on + strand at 3109.154 kb on - strand at 3109.187 kb on + strand at 3109.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 150 mM remove 3,107,361 - +0.4 3,107,361 - -0.3 3,107,361 - -0.3 3,107,361 - +0.3 3,107,445 - -0.1 3,107,490 + -0.8 3,107,526 + -2.5 3,107,526 + -0.4 3,107,526 + -2.2 3,107,526 + -1.2 3,107,526 + -0.3 3,107,527 - -1.7 3,107,527 - -2.7 3,107,538 + -0.6 3,107,538 + -0.8 3,107,539 - -1.5 3,107,539 - -2.4 3,107,539 - -1.2 3,107,539 - -1.7 3,107,547 + ABZR86_RS13935 0.10 -0.2 3,107,547 + ABZR86_RS13935 0.10 +0.0 3,107,548 - ABZR86_RS13935 0.10 -0.8 3,107,982 + ABZR86_RS13935 0.58 -1.2 3,107,982 + ABZR86_RS13935 0.58 -1.3 3,107,983 - ABZR86_RS13935 0.58 -1.8 3,107,983 - ABZR86_RS13935 0.58 +0.7 3,108,287 + -0.4 3,108,288 - -1.4 3,108,339 + -1.0 3,108,339 + -0.3 3,108,339 + -0.4 3,108,339 + -1.1 3,108,339 + -0.2 3,108,339 + -1.9 3,108,339 + -2.0 3,108,339 + -2.0 3,108,340 - -0.1 3,108,340 - +0.2 3,108,340 - +0.7 3,108,340 - +0.3 3,108,348 + -1.6 3,108,348 + -0.9 3,108,348 + -0.6 3,108,348 + -1.2 3,108,348 + -0.5 3,108,348 + -2.7 3,108,348 + -0.8 3,108,348 + -1.0 3,108,349 - -1.5 3,108,349 - +0.2 3,108,349 - -0.1 3,108,349 - -0.3 3,108,349 - -0.2 3,108,349 - +0.8 3,108,349 - -0.1 3,108,349 - -0.4 3,108,449 - -0.4 3,108,449 - +0.7 3,108,464 + -0.1 3,108,464 + -0.1 3,108,465 - +0.5 3,108,465 - +0.5 3,108,465 - +0.6 3,108,465 - -0.0 3,108,496 + -0.5 3,108,496 + +0.7 3,108,497 - +0.7 3,108,527 + -0.3 3,108,543 + +0.1 3,108,543 + -0.0 3,108,543 + +0.2 3,108,544 - +0.5 3,108,578 - +0.3 3,108,965 + ABZR86_RS13940 0.87 -0.2 3,108,965 + ABZR86_RS13940 0.87 -1.2 3,108,965 + ABZR86_RS13940 0.87 -0.0 3,108,966 - ABZR86_RS13940 0.87 +0.6 3,108,966 - ABZR86_RS13940 0.87 -1.5 3,108,966 - ABZR86_RS13940 0.87 +0.3 3,109,069 - -0.5 3,109,098 - +0.3 3,109,106 + -0.4 3,109,106 + -0.8 3,109,107 - -0.2 3,109,112 + +0.2 3,109,113 - +0.6 3,109,153 + +0.2 3,109,154 - -0.6 3,109,187 + -0.6 3,109,187 + -0.1
Or see this region's nucleotide sequence