Experiment: nitrate 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS02175 and ABZR86_RS02180 are separated by 6 nucleotides ABZR86_RS02180 and ABZR86_RS02185 are separated by 10 nucleotides ABZR86_RS02185 and ABZR86_RS02190 overlap by 4 nucleotides ABZR86_RS02190 and ABZR86_RS02195 are separated by 138 nucleotides
ABZR86_RS02175: ABZR86_RS02175 - LysR family transcriptional regulator, at 469,472 to 470,380
_RS02175
ABZR86_RS02180: ABZR86_RS02180 - high frequency lysogenization protein HflD, at 470,387 to 471,010
_RS02180
ABZR86_RS02185: ABZR86_RS02185 - tRNA 2-thiouridine(34) synthase MnmA, at 471,021 to 472,151
_RS02185
ABZR86_RS02190: ABZR86_RS02190 - NUDIX hydrolase, at 472,148 to 472,642
_RS02190
ABZR86_RS02195: ABZR86_RS02195 - ATP-dependent Clp protease adapter ClpS, at 472,781 to 473,107
_RS02195
Position (kb) 471
472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 470.172 kb on - strand, within ABZR86_RS02175 at 470.172 kb on - strand, within ABZR86_RS02175 at 470.274 kb on - strand, within ABZR86_RS02175 at 470.274 kb on - strand, within ABZR86_RS02175 at 470.274 kb on - strand, within ABZR86_RS02175 at 470.451 kb on + strand, within ABZR86_RS02180 at 470.452 kb on - strand, within ABZR86_RS02180 at 470.619 kb on + strand, within ABZR86_RS02180 at 470.619 kb on + strand, within ABZR86_RS02180 at 470.619 kb on + strand, within ABZR86_RS02180 at 470.620 kb on - strand, within ABZR86_RS02180 at 470.620 kb on - strand, within ABZR86_RS02180 at 470.857 kb on - strand, within ABZR86_RS02180 at 470.857 kb on - strand, within ABZR86_RS02180 at 471.015 kb on + strand at 471.015 kb on + strand at 471.015 kb on + strand at 471.015 kb on + strand at 471.015 kb on + strand at 471.015 kb on + strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.016 kb on - strand at 471.074 kb on - strand at 471.127 kb on + strand at 471.135 kb on + strand, within ABZR86_RS02185 at 471.355 kb on + strand, within ABZR86_RS02185 at 471.356 kb on - strand, within ABZR86_RS02185 at 471.391 kb on + strand, within ABZR86_RS02185 at 471.392 kb on - strand, within ABZR86_RS02185 at 471.392 kb on - strand, within ABZR86_RS02185 at 471.457 kb on + strand, within ABZR86_RS02185 at 471.457 kb on + strand, within ABZR86_RS02185 at 471.457 kb on + strand, within ABZR86_RS02185 at 471.457 kb on + strand, within ABZR86_RS02185 at 471.457 kb on + strand, within ABZR86_RS02185 at 471.458 kb on - strand, within ABZR86_RS02185 at 471.458 kb on - strand, within ABZR86_RS02185 at 471.458 kb on - strand, within ABZR86_RS02185 at 471.458 kb on - strand, within ABZR86_RS02185 at 471.458 kb on - strand, within ABZR86_RS02185 at 471.460 kb on + strand, within ABZR86_RS02185 at 471.460 kb on + strand, within ABZR86_RS02185 at 471.460 kb on + strand, within ABZR86_RS02185 at 471.460 kb on + strand, within ABZR86_RS02185 at 471.461 kb on - strand, within ABZR86_RS02185 at 471.461 kb on - strand, within ABZR86_RS02185 at 471.461 kb on - strand, within ABZR86_RS02185 at 471.461 kb on - strand, within ABZR86_RS02185 at 471.461 kb on - strand, within ABZR86_RS02185 at 471.532 kb on + strand, within ABZR86_RS02185 at 471.532 kb on + strand, within ABZR86_RS02185 at 471.532 kb on + strand, within ABZR86_RS02185 at 471.532 kb on + strand, within ABZR86_RS02185 at 471.533 kb on - strand, within ABZR86_RS02185 at 471.533 kb on - strand, within ABZR86_RS02185 at 471.533 kb on - strand, within ABZR86_RS02185 at 471.712 kb on + strand, within ABZR86_RS02185 at 471.712 kb on + strand, within ABZR86_RS02185 at 471.712 kb on + strand, within ABZR86_RS02185 at 471.713 kb on - strand, within ABZR86_RS02185 at 471.713 kb on - strand, within ABZR86_RS02185 at 471.713 kb on - strand, within ABZR86_RS02185 at 471.754 kb on + strand, within ABZR86_RS02185 at 471.785 kb on - strand, within ABZR86_RS02185 at 471.899 kb on - strand, within ABZR86_RS02185 at 472.406 kb on + strand, within ABZR86_RS02190 at 472.407 kb on - strand, within ABZR86_RS02190 at 472.407 kb on - strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.518 kb on + strand, within ABZR86_RS02190 at 472.519 kb on - strand, within ABZR86_RS02190 at 472.519 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.558 kb on - strand, within ABZR86_RS02190 at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.653 kb on + strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.654 kb on - strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.655 kb on + strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.656 kb on - strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.710 kb on + strand at 472.711 kb on - strand at 472.711 kb on - strand at 472.748 kb on + strand at 472.749 kb on - strand at 472.749 kb on - strand at 472.749 kb on - strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.750 kb on + strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.751 kb on - strand at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.984 kb on + strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 472.985 kb on - strand, within ABZR86_RS02195 at 473.057 kb on + strand, within ABZR86_RS02195 at 473.058 kb on - strand, within ABZR86_RS02195 at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.085 kb on + strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.086 kb on - strand at 473.092 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 150 mM remove 470,172 - ABZR86_RS02175 0.77 +0.0 470,172 - ABZR86_RS02175 0.77 -0.1 470,274 - ABZR86_RS02175 0.88 -0.1 470,274 - ABZR86_RS02175 0.88 -1.0 470,274 - ABZR86_RS02175 0.88 -0.0 470,451 + ABZR86_RS02180 0.10 -0.3 470,452 - ABZR86_RS02180 0.10 -0.8 470,619 + ABZR86_RS02180 0.37 +0.0 470,619 + ABZR86_RS02180 0.37 -0.5 470,619 + ABZR86_RS02180 0.37 -2.3 470,620 - ABZR86_RS02180 0.37 -1.4 470,620 - ABZR86_RS02180 0.37 -0.4 470,857 - ABZR86_RS02180 0.75 -0.3 470,857 - ABZR86_RS02180 0.75 -0.8 471,015 + -0.3 471,015 + +0.0 471,015 + -1.1 471,015 + -0.4 471,015 + -0.1 471,015 + -0.7 471,016 - +0.1 471,016 - -1.0 471,016 - -1.3 471,016 - +0.1 471,016 - +0.2 471,016 - -0.1 471,016 - -0.4 471,074 - -0.1 471,127 + +0.5 471,135 + ABZR86_RS02185 0.10 -0.7 471,355 + ABZR86_RS02185 0.30 -0.6 471,356 - ABZR86_RS02185 0.30 +0.2 471,391 + ABZR86_RS02185 0.33 -2.8 471,392 - ABZR86_RS02185 0.33 -0.6 471,392 - ABZR86_RS02185 0.33 -1.9 471,457 + ABZR86_RS02185 0.39 -1.0 471,457 + ABZR86_RS02185 0.39 -1.9 471,457 + ABZR86_RS02185 0.39 +0.5 471,457 + ABZR86_RS02185 0.39 -0.4 471,457 + ABZR86_RS02185 0.39 -1.3 471,458 - ABZR86_RS02185 0.39 -1.4 471,458 - ABZR86_RS02185 0.39 -0.6 471,458 - ABZR86_RS02185 0.39 +0.4 471,458 - ABZR86_RS02185 0.39 -1.3 471,458 - ABZR86_RS02185 0.39 -1.2 471,460 + ABZR86_RS02185 0.39 -0.4 471,460 + ABZR86_RS02185 0.39 -1.5 471,460 + ABZR86_RS02185 0.39 -1.4 471,460 + ABZR86_RS02185 0.39 +0.1 471,461 - ABZR86_RS02185 0.39 -1.0 471,461 - ABZR86_RS02185 0.39 -0.9 471,461 - ABZR86_RS02185 0.39 -1.2 471,461 - ABZR86_RS02185 0.39 -0.9 471,461 - ABZR86_RS02185 0.39 -0.8 471,532 + ABZR86_RS02185 0.45 -0.6 471,532 + ABZR86_RS02185 0.45 -0.4 471,532 + ABZR86_RS02185 0.45 +0.6 471,532 + ABZR86_RS02185 0.45 +1.8 471,533 - ABZR86_RS02185 0.45 -1.6 471,533 - ABZR86_RS02185 0.45 -1.4 471,533 - ABZR86_RS02185 0.45 -0.0 471,712 + ABZR86_RS02185 0.61 -0.7 471,712 + ABZR86_RS02185 0.61 -0.5 471,712 + ABZR86_RS02185 0.61 -1.2 471,713 - ABZR86_RS02185 0.61 -0.0 471,713 - ABZR86_RS02185 0.61 +0.4 471,713 - ABZR86_RS02185 0.61 -0.1 471,754 + ABZR86_RS02185 0.65 -0.4 471,785 - ABZR86_RS02185 0.68 -0.7 471,899 - ABZR86_RS02185 0.78 -2.1 472,406 + ABZR86_RS02190 0.52 -0.7 472,407 - ABZR86_RS02190 0.52 +0.7 472,407 - ABZR86_RS02190 0.52 -0.5 472,518 + ABZR86_RS02190 0.75 -0.4 472,518 + ABZR86_RS02190 0.75 +0.2 472,518 + ABZR86_RS02190 0.75 +0.2 472,518 + ABZR86_RS02190 0.75 +0.1 472,519 - ABZR86_RS02190 0.75 +0.4 472,519 - ABZR86_RS02190 0.75 +0.0 472,558 - ABZR86_RS02190 0.83 +0.0 472,558 - ABZR86_RS02190 0.83 +0.3 472,558 - ABZR86_RS02190 0.83 +1.0 472,653 + +0.3 472,653 + -0.0 472,653 + +0.5 472,653 + +0.1 472,653 + -0.2 472,653 + -0.3 472,654 - +0.1 472,654 - +0.3 472,654 - +0.3 472,654 - -0.1 472,655 + -0.4 472,655 + +0.2 472,655 + +0.5 472,655 + -0.2 472,655 + -0.9 472,655 + -0.8 472,655 + +0.2 472,655 + +0.0 472,655 + -0.1 472,656 - -0.5 472,656 - -0.7 472,656 - +0.2 472,656 - +0.1 472,656 - -0.2 472,656 - -0.1 472,656 - -0.5 472,656 - +0.0 472,656 - -0.5 472,656 - +0.0 472,656 - +0.1 472,656 - -0.8 472,656 - +0.1 472,656 - -0.3 472,656 - +0.5 472,656 - -0.7 472,710 + +1.0 472,710 + +1.1 472,710 + -0.2 472,710 + -0.0 472,711 - +0.9 472,711 - -0.1 472,748 + +0.7 472,749 - -0.4 472,749 - +0.1 472,749 - -0.0 472,750 + -0.1 472,750 + -0.2 472,750 + +2.0 472,750 + -0.0 472,750 + -0.6 472,751 - -2.2 472,751 - -0.7 472,751 - -3.3 472,751 - -1.6 472,751 - +0.1 472,984 + ABZR86_RS02195 0.62 -0.5 472,984 + ABZR86_RS02195 0.62 -2.0 472,984 + ABZR86_RS02195 0.62 -1.5 472,984 + ABZR86_RS02195 0.62 -1.5 472,985 - ABZR86_RS02195 0.62 -1.6 472,985 - ABZR86_RS02195 0.62 -0.6 472,985 - ABZR86_RS02195 0.62 -1.2 472,985 - ABZR86_RS02195 0.62 -1.6 473,057 + ABZR86_RS02195 0.84 -2.4 473,058 - ABZR86_RS02195 0.85 -2.0 473,085 + -0.8 473,085 + -0.5 473,085 + -1.3 473,085 + -1.8 473,085 + -1.6 473,085 + -0.6 473,085 + -1.0 473,085 + -1.7 473,086 - -0.5 473,086 - -1.6 473,086 - -2.4 473,086 - -1.0 473,092 + -0.9
Or see this region's nucleotide sequence