Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3839 and Shew_3840 are separated by 35 nucleotides Shew_3840 and Shew_3841 are separated by 145 nucleotides Shew_3841 and Shew_3842 are separated by 210 nucleotides
Shew_3839: Shew_3839 - glucosamine--fructose-6-phosphate aminotransferase, isomerizing (RefSeq), at 4,569,026 to 4,570,855
_3839
Shew_3840: Shew_3840 - DeoR family transcriptional regulator (RefSeq), at 4,570,891 to 4,571,661
_3840
Shew_3841: Shew_3841 - TonB-dependent siderophore receptor (RefSeq), at 4,571,807 to 4,573,945
_3841
Shew_3842: Shew_3842 - UDP-N-acetylglucosamine pyrophosphorylase (RefSeq), at 4,574,156 to 4,575,520
_3842
Position (kb)
4571
4572
4573
4574 Strain fitness (log2 ratio)
-2
-1
0
1 at 4571.561 kb on - strand, within Shew_3840 at 4571.790 kb on - strand at 4572.025 kb on + strand, within Shew_3841 at 4572.275 kb on + strand, within Shew_3841 at 4572.901 kb on + strand, within Shew_3841 at 4573.035 kb on - strand, within Shew_3841 at 4573.059 kb on - strand, within Shew_3841 at 4573.091 kb on - strand, within Shew_3841 at 4573.158 kb on + strand, within Shew_3841 at 4573.166 kb on - strand, within Shew_3841 at 4573.182 kb on - strand, within Shew_3841 at 4573.192 kb on + strand, within Shew_3841 at 4573.192 kb on + strand, within Shew_3841 at 4573.200 kb on - strand, within Shew_3841 at 4573.320 kb on + strand, within Shew_3841 at 4573.328 kb on - strand, within Shew_3841 at 4573.783 kb on + strand at 4573.841 kb on - strand at 4574.030 kb on + strand at 4574.030 kb on + strand at 4574.030 kb on + strand at 4574.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,571,561 - Shew_3840 0.87 +0.3 4,571,790 - -0.8 4,572,025 + Shew_3841 0.10 -0.7 4,572,275 + Shew_3841 0.22 +0.8 4,572,901 + Shew_3841 0.51 +0.0 4,573,035 - Shew_3841 0.57 -2.3 4,573,059 - Shew_3841 0.59 -0.7 4,573,091 - Shew_3841 0.60 -0.1 4,573,158 + Shew_3841 0.63 -0.5 4,573,166 - Shew_3841 0.64 -1.4 4,573,182 - Shew_3841 0.64 -2.7 4,573,192 + Shew_3841 0.65 -0.5 4,573,192 + Shew_3841 0.65 -0.7 4,573,200 - Shew_3841 0.65 -0.2 4,573,320 + Shew_3841 0.71 -0.2 4,573,328 - Shew_3841 0.71 -0.5 4,573,783 + -1.0 4,573,841 - -0.6 4,574,030 + +0.2 4,574,030 + -2.3 4,574,030 + -0.1 4,574,038 - -0.1
Or see this region's nucleotide sequence