Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3776 and Shew_3777 are separated by 127 nucleotides Shew_3777 and Shew_3778 overlap by 4 nucleotides Shew_3778 and Shew_3779 are separated by 8 nucleotides
Shew_3776: Shew_3776 - AMP-dependent synthetase and ligase (RefSeq), at 4,494,661 to 4,496,457
_3776
Shew_3777: Shew_3777 - hypothetical protein (RefSeq), at 4,496,585 to 4,497,847
_3777
Shew_3778: Shew_3778 - ABC transporter-related protein (RefSeq), at 4,497,844 to 4,498,695
_3778
Shew_3779: Shew_3779 - GntR family transcriptional regulator (RefSeq), at 4,498,704 to 4,499,084
_3779
Position (kb)
4496
4497
4498 Strain fitness (log2 ratio)
-1
0
1 at 4495.595 kb on + strand, within Shew_3776 at 4495.600 kb on + strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.608 kb on - strand, within Shew_3776 at 4495.613 kb on + strand, within Shew_3776 at 4495.621 kb on - strand, within Shew_3776 at 4495.621 kb on - strand, within Shew_3776 at 4495.658 kb on - strand, within Shew_3776 at 4496.090 kb on - strand, within Shew_3776 at 4496.241 kb on - strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.272 kb on + strand, within Shew_3776 at 4496.280 kb on - strand at 4496.371 kb on + strand at 4496.596 kb on - strand at 4496.789 kb on + strand, within Shew_3777 at 4496.789 kb on + strand, within Shew_3777 at 4496.925 kb on + strand, within Shew_3777 at 4496.995 kb on - strand, within Shew_3777 at 4496.996 kb on + strand, within Shew_3777 at 4497.041 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.054 kb on + strand, within Shew_3777 at 4497.067 kb on - strand, within Shew_3777 at 4497.072 kb on - strand, within Shew_3777 at 4497.163 kb on + strand, within Shew_3777 at 4497.319 kb on - strand, within Shew_3777 at 4497.455 kb on - strand, within Shew_3777 at 4497.509 kb on + strand, within Shew_3777 at 4497.530 kb on + strand, within Shew_3777 at 4497.588 kb on - strand, within Shew_3777 at 4497.614 kb on + strand, within Shew_3777 at 4497.614 kb on + strand, within Shew_3777 at 4497.617 kb on - strand, within Shew_3777 at 4497.769 kb on + strand at 4497.845 kb on + strand at 4498.001 kb on - strand, within Shew_3778 at 4498.001 kb on - strand, within Shew_3778 at 4498.026 kb on - strand, within Shew_3778 at 4498.066 kb on + strand, within Shew_3778 at 4498.190 kb on + strand, within Shew_3778 at 4498.247 kb on + strand, within Shew_3778 at 4498.270 kb on + strand, within Shew_3778 at 4498.281 kb on - strand, within Shew_3778 at 4498.296 kb on - strand, within Shew_3778 at 4498.388 kb on - strand, within Shew_3778 at 4498.397 kb on + strand, within Shew_3778
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,495,595 + Shew_3776 0.52 -0.5 4,495,600 + Shew_3776 0.52 -0.4 4,495,608 - Shew_3776 0.53 -0.3 4,495,608 - Shew_3776 0.53 -0.3 4,495,608 - Shew_3776 0.53 -0.1 4,495,613 + Shew_3776 0.53 +0.1 4,495,621 - Shew_3776 0.53 -0.3 4,495,621 - Shew_3776 0.53 -0.1 4,495,658 - Shew_3776 0.55 -0.6 4,496,090 - Shew_3776 0.80 -1.4 4,496,241 - Shew_3776 0.88 -0.1 4,496,272 + Shew_3776 0.90 -0.7 4,496,272 + Shew_3776 0.90 -1.1 4,496,272 + Shew_3776 0.90 -1.3 4,496,280 - +0.1 4,496,371 + -1.0 4,496,596 - +0.2 4,496,789 + Shew_3777 0.16 -0.9 4,496,789 + Shew_3777 0.16 -0.6 4,496,925 + Shew_3777 0.27 -0.3 4,496,995 - Shew_3777 0.32 +0.1 4,496,996 + Shew_3777 0.33 -0.1 4,497,041 + Shew_3777 0.36 +0.1 4,497,054 + Shew_3777 0.37 +0.5 4,497,054 + Shew_3777 0.37 +0.7 4,497,054 + Shew_3777 0.37 -0.1 4,497,067 - Shew_3777 0.38 -1.3 4,497,072 - Shew_3777 0.39 -0.1 4,497,163 + Shew_3777 0.46 -0.2 4,497,319 - Shew_3777 0.58 +1.0 4,497,455 - Shew_3777 0.69 +0.7 4,497,509 + Shew_3777 0.73 +0.0 4,497,530 + Shew_3777 0.75 -0.1 4,497,588 - Shew_3777 0.79 -0.5 4,497,614 + Shew_3777 0.81 +0.2 4,497,614 + Shew_3777 0.81 +0.6 4,497,617 - Shew_3777 0.82 +0.2 4,497,769 + -0.1 4,497,845 + +1.1 4,498,001 - Shew_3778 0.18 +1.1 4,498,001 - Shew_3778 0.18 +0.6 4,498,026 - Shew_3778 0.21 +0.1 4,498,066 + Shew_3778 0.26 +0.6 4,498,190 + Shew_3778 0.41 +0.1 4,498,247 + Shew_3778 0.47 +0.6 4,498,270 + Shew_3778 0.50 +0.5 4,498,281 - Shew_3778 0.51 +1.2 4,498,296 - Shew_3778 0.53 +0.1 4,498,388 - Shew_3778 0.64 +0.1 4,498,397 + Shew_3778 0.65 +0.6
Or see this region's nucleotide sequence