Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3691 and coaD are separated by 10 nucleotides coaD and Shew_3693 are separated by 290 nucleotides Shew_3693 and Shew_3694 are separated by 45 nucleotides
Shew_3691: Shew_3691 - hypothetical protein (RefSeq), at 4,395,002 to 4,396,507
_3691
Shew_3692: coaD - phosphopantetheine adenylyltransferase (RefSeq), at 4,396,518 to 4,396,994
coaD
Shew_3693: Shew_3693 - ADP-heptose--lipooligosaccharide heptosyltransferase III (RefSeq), at 4,397,285 to 4,398,310
_3693
Shew_3694: Shew_3694 - ADP-L-glycero-D-manno-heptose-6-epimerase (RefSeq), at 4,398,356 to 4,399,309
_3694
Position (kb)
4397
4398
4399 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4397.244 kb on - strand at 4397.338 kb on + strand at 4397.346 kb on - strand at 4397.544 kb on - strand, within Shew_3693 at 4397.639 kb on - strand, within Shew_3693 at 4397.689 kb on + strand, within Shew_3693 at 4397.789 kb on + strand, within Shew_3693 at 4397.917 kb on + strand, within Shew_3693 at 4397.917 kb on + strand, within Shew_3693 at 4397.970 kb on + strand, within Shew_3693 at 4398.167 kb on - strand, within Shew_3693 at 4398.222 kb on + strand at 4398.664 kb on - strand, within Shew_3694 at 4398.850 kb on - strand, within Shew_3694 at 4398.869 kb on - strand, within Shew_3694 at 4399.023 kb on + strand, within Shew_3694 at 4399.066 kb on - strand, within Shew_3694 at 4399.165 kb on + strand, within Shew_3694 at 4399.178 kb on + strand, within Shew_3694 at 4399.208 kb on - strand, within Shew_3694 at 4399.210 kb on + strand, within Shew_3694 at 4399.210 kb on + strand, within Shew_3694 at 4399.210 kb on + strand, within Shew_3694 at 4399.218 kb on - strand at 4399.218 kb on - strand at 4399.218 kb on - strand at 4399.223 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,397,244 - +2.5 4,397,338 + +2.6 4,397,346 - +2.7 4,397,544 - Shew_3693 0.25 +2.6 4,397,639 - Shew_3693 0.35 +2.5 4,397,689 + Shew_3693 0.39 +2.8 4,397,789 + Shew_3693 0.49 +3.6 4,397,917 + Shew_3693 0.62 +2.4 4,397,917 + Shew_3693 0.62 +2.6 4,397,970 + Shew_3693 0.67 +2.8 4,398,167 - Shew_3693 0.86 +2.8 4,398,222 + +2.7 4,398,664 - Shew_3694 0.32 +2.1 4,398,850 - Shew_3694 0.52 +2.3 4,398,869 - Shew_3694 0.54 +0.7 4,399,023 + Shew_3694 0.70 +2.9 4,399,066 - Shew_3694 0.74 +3.1 4,399,165 + Shew_3694 0.85 +2.6 4,399,178 + Shew_3694 0.86 +1.9 4,399,208 - Shew_3694 0.89 +2.1 4,399,210 + Shew_3694 0.90 +2.7 4,399,210 + Shew_3694 0.90 +2.8 4,399,210 + Shew_3694 0.90 +2.2 4,399,218 - +2.4 4,399,218 - +2.2 4,399,218 - +2.6 4,399,223 + +2.7
Or see this region's nucleotide sequence