Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3630 and Shew_3631 are separated by 58 nucleotides Shew_3631 and Shew_3632 overlap by 8 nucleotides Shew_3632 and Shew_3633 are separated by 492 nucleotides Shew_3633 and Shew_3634 are separated by 87 nucleotides
Shew_3630: Shew_3630 - bioH protein (RefSeq), at 4,328,079 to 4,328,858
_3630
Shew_3631: Shew_3631 - hypothetical protein (RefSeq), at 4,328,917 to 4,329,411
_3631
Shew_3632: Shew_3632 - sulfatase (RefSeq), at 4,329,404 to 4,331,392
_3632
Shew_3633: Shew_3633 - hypothetical protein (RefSeq), at 4,331,885 to 4,332,103
_3633
Shew_3634: Shew_3634 - RNA-binding S1 domain-containing protein (RefSeq), at 4,332,191 to 4,334,554
_3634
Position (kb)
4329
4330
4331
4332 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4328.570 kb on - strand, within Shew_3630 at 4328.834 kb on - strand at 4329.085 kb on - strand, within Shew_3631 at 4329.289 kb on + strand, within Shew_3631 at 4329.434 kb on + strand at 4329.457 kb on - strand at 4329.548 kb on + strand at 4329.751 kb on - strand, within Shew_3632 at 4329.762 kb on - strand, within Shew_3632 at 4329.838 kb on + strand, within Shew_3632 at 4329.850 kb on + strand, within Shew_3632 at 4330.063 kb on - strand, within Shew_3632 at 4330.089 kb on + strand, within Shew_3632 at 4330.224 kb on - strand, within Shew_3632 at 4330.522 kb on - strand, within Shew_3632 at 4330.522 kb on - strand, within Shew_3632 at 4330.672 kb on + strand, within Shew_3632 at 4330.680 kb on - strand, within Shew_3632 at 4330.815 kb on - strand, within Shew_3632 at 4330.956 kb on + strand, within Shew_3632 at 4331.175 kb on + strand, within Shew_3632 at 4331.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,328,570 - Shew_3630 0.63 -0.0 4,328,834 - -5.2 4,329,085 - Shew_3631 0.34 +0.4 4,329,289 + Shew_3631 0.75 +1.3 4,329,434 + +0.5 4,329,457 - +1.0 4,329,548 + +0.3 4,329,751 - Shew_3632 0.17 +1.2 4,329,762 - Shew_3632 0.18 +1.4 4,329,838 + Shew_3632 0.22 +2.1 4,329,850 + Shew_3632 0.22 +2.0 4,330,063 - Shew_3632 0.33 +1.7 4,330,089 + Shew_3632 0.34 +0.1 4,330,224 - Shew_3632 0.41 +2.1 4,330,522 - Shew_3632 0.56 +2.2 4,330,522 - Shew_3632 0.56 +0.9 4,330,672 + Shew_3632 0.64 +1.9 4,330,680 - Shew_3632 0.64 +1.5 4,330,815 - Shew_3632 0.71 +2.1 4,330,956 + Shew_3632 0.78 +1.7 4,331,175 + Shew_3632 0.89 +2.2 4,331,743 + -3.0
Or see this region's nucleotide sequence