Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3521 and Shew_3522 overlap by 10 nucleotides Shew_3522 and Shew_3523 are separated by 32 nucleotides Shew_3523 and Shew_3524 overlap by 1 nucleotides Shew_3524 and fabG overlap by 4 nucleotides
Shew_3521: Shew_3521 - tryptophan halogenase (RefSeq), at 4,208,445 to 4,209,719
_3521
Shew_3522: Shew_3522 - hypothetical protein (RefSeq), at 4,209,710 to 4,210,282
_3522
Shew_3523: Shew_3523 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 4,210,315 to 4,211,520
_3523
Shew_3524: Shew_3524 - Beta-hydroxyacyl-(acyl-carrier-protein) dehydratase, FabA/FabZ (RefSeq), at 4,211,520 to 4,212,113
_3524
Shew_3525: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 4,212,110 to 4,212,835
fabG
Position (kb)
4210
4211
4212 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4209.411 kb on + strand, within Shew_3521 at 4209.615 kb on - strand at 4209.638 kb on - strand at 4209.809 kb on + strand, within Shew_3522 at 4209.876 kb on + strand, within Shew_3522 at 4210.277 kb on + strand at 4210.477 kb on - strand, within Shew_3523 at 4210.604 kb on + strand, within Shew_3523 at 4210.716 kb on + strand, within Shew_3523 at 4210.984 kb on + strand, within Shew_3523 at 4211.150 kb on + strand, within Shew_3523 at 4211.263 kb on + strand, within Shew_3523 at 4211.578 kb on - strand at 4211.681 kb on - strand, within Shew_3524 at 4211.778 kb on + strand, within Shew_3524 at 4211.786 kb on - strand, within Shew_3524 at 4212.026 kb on + strand, within Shew_3524 at 4212.173 kb on - strand at 4212.173 kb on - strand at 4212.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,209,411 + Shew_3521 0.76 -0.1 4,209,615 - +0.1 4,209,638 - +0.4 4,209,809 + Shew_3522 0.17 -0.2 4,209,876 + Shew_3522 0.29 +2.4 4,210,277 + -1.7 4,210,477 - Shew_3523 0.13 -1.7 4,210,604 + Shew_3523 0.24 +0.3 4,210,716 + Shew_3523 0.33 +0.3 4,210,984 + Shew_3523 0.55 +0.8 4,211,150 + Shew_3523 0.69 +0.2 4,211,263 + Shew_3523 0.79 +0.4 4,211,578 - +1.0 4,211,681 - Shew_3524 0.27 +1.1 4,211,778 + Shew_3524 0.43 -0.8 4,211,786 - Shew_3524 0.45 -2.4 4,212,026 + Shew_3524 0.85 -2.3 4,212,173 - +0.6 4,212,173 - -2.4 4,212,173 - +0.2
Or see this region's nucleotide sequence