Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3499 and Shew_3500 are separated by 34 nucleotides Shew_3500 and Shew_3501 are separated by 68 nucleotides Shew_3501 and Shew_3502 are separated by 341 nucleotides
Shew_3499: Shew_3499 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,184,648 to 4,186,753
_3499
Shew_3500: Shew_3500 - putative endoribonuclease L-PSP (RefSeq), at 4,186,788 to 4,187,171
_3500
Shew_3501: Shew_3501 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 4,187,240 to 4,187,944
_3501
Shew_3502: Shew_3502 - AMP-dependent synthetase and ligase (RefSeq), at 4,188,286 to 4,189,941
_3502
Position (kb)
4187
4188 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4186.480 kb on + strand, within Shew_3499 at 4186.560 kb on - strand at 4187.011 kb on - strand, within Shew_3500 at 4187.095 kb on - strand, within Shew_3500 at 4187.449 kb on + strand, within Shew_3501 at 4187.771 kb on - strand, within Shew_3501 at 4187.803 kb on + strand, within Shew_3501 at 4187.803 kb on + strand, within Shew_3501 at 4187.811 kb on - strand, within Shew_3501 at 4187.813 kb on + strand, within Shew_3501 at 4187.818 kb on + strand, within Shew_3501 at 4188.273 kb on - strand at 4188.297 kb on + strand at 4188.414 kb on - strand at 4188.452 kb on + strand, within Shew_3502 at 4188.460 kb on - strand, within Shew_3502 at 4188.507 kb on - strand, within Shew_3502 at 4188.535 kb on + strand, within Shew_3502 at 4188.552 kb on + strand, within Shew_3502 at 4188.774 kb on - strand, within Shew_3502 at 4188.783 kb on + strand, within Shew_3502 at 4188.822 kb on + strand, within Shew_3502 at 4188.843 kb on - strand, within Shew_3502 at 4188.843 kb on - strand, within Shew_3502 at 4188.885 kb on + strand, within Shew_3502
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 4,186,480 + Shew_3499 0.87 -1.2 4,186,560 - -1.4 4,187,011 - Shew_3500 0.58 +0.8 4,187,095 - Shew_3500 0.80 +0.9 4,187,449 + Shew_3501 0.30 +0.7 4,187,771 - Shew_3501 0.75 -0.0 4,187,803 + Shew_3501 0.80 -0.5 4,187,803 + Shew_3501 0.80 +0.1 4,187,811 - Shew_3501 0.81 -0.0 4,187,813 + Shew_3501 0.81 +0.1 4,187,818 + Shew_3501 0.82 +0.1 4,188,273 - +0.0 4,188,297 + +0.4 4,188,414 - +0.3 4,188,452 + Shew_3502 0.10 -0.2 4,188,460 - Shew_3502 0.11 +1.0 4,188,507 - Shew_3502 0.13 +0.3 4,188,535 + Shew_3502 0.15 +0.4 4,188,552 + Shew_3502 0.16 +0.9 4,188,774 - Shew_3502 0.29 +1.8 4,188,783 + Shew_3502 0.30 -0.9 4,188,822 + Shew_3502 0.32 -0.1 4,188,843 - Shew_3502 0.34 +0.1 4,188,843 - Shew_3502 0.34 -2.0 4,188,885 + Shew_3502 0.36 -0.1
Or see this region's nucleotide sequence