Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3206 and napG are separated by 58 nucleotides napG and napH overlap by 4 nucleotides napH and Shew_3209 overlap by 4 nucleotides Shew_3209 and Shew_3210 are separated by 529 nucleotides
Shew_3206: Shew_3206 - nitrate reductase (RefSeq), at 3,823,608 to 3,826,091
_3206
Shew_3207: napG - quinol dehydrogenase periplasmic component (RefSeq), at 3,826,150 to 3,826,881
napG
Shew_3208: napH - quinol dehydrogenase membrane component (RefSeq), at 3,826,878 to 3,827,777
napH
Shew_3209: Shew_3209 - nitrate reductase cytochrome c-type subunit (NapB) (RefSeq), at 3,827,774 to 3,828,196
_3209
Shew_3210: Shew_3210 - hypothetical protein (RefSeq), at 3,828,726 to 3,829,013
_3210
Position (kb)
3826
3827
3828 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3826.027 kb on + strand at 3826.133 kb on + strand at 3826.366 kb on + strand, within napG at 3826.371 kb on + strand, within napG at 3826.372 kb on - strand, within napG at 3826.379 kb on - strand, within napG at 3826.414 kb on - strand, within napG at 3826.436 kb on + strand, within napG at 3826.474 kb on + strand, within napG at 3826.482 kb on - strand, within napG at 3826.482 kb on - strand, within napG at 3826.700 kb on - strand, within napG at 3826.776 kb on + strand, within napG at 3826.847 kb on + strand at 3827.151 kb on + strand, within napH at 3827.398 kb on - strand, within napH at 3827.503 kb on - strand, within napH at 3827.505 kb on + strand, within napH at 3827.538 kb on - strand, within napH at 3827.615 kb on + strand, within napH at 3827.911 kb on - strand, within Shew_3209 at 3827.969 kb on + strand, within Shew_3209 at 3828.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,826,027 + +0.8 3,826,133 + +0.3 3,826,366 + napG Shew_3207 0.30 -2.0 3,826,371 + napG Shew_3207 0.30 +0.6 3,826,372 - napG Shew_3207 0.30 -0.0 3,826,379 - napG Shew_3207 0.31 -0.7 3,826,414 - napG Shew_3207 0.36 +0.2 3,826,436 + napG Shew_3207 0.39 -0.4 3,826,474 + napG Shew_3207 0.44 +0.3 3,826,482 - napG Shew_3207 0.45 -0.7 3,826,482 - napG Shew_3207 0.45 -0.9 3,826,700 - napG Shew_3207 0.75 +0.2 3,826,776 + napG Shew_3207 0.86 +0.1 3,826,847 + +0.5 3,827,151 + napH Shew_3208 0.30 +0.3 3,827,398 - napH Shew_3208 0.58 +1.0 3,827,503 - napH Shew_3208 0.69 -1.2 3,827,505 + napH Shew_3208 0.70 +0.6 3,827,538 - napH Shew_3208 0.73 -0.2 3,827,615 + napH Shew_3208 0.82 -1.3 3,827,911 - Shew_3209 0.32 +2.0 3,827,969 + Shew_3209 0.46 +0.4 3,828,642 + -0.5
Or see this region's nucleotide sequence