Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3015 and Shew_3016 are separated by 86 nucleotides Shew_3016 and Shew_3017 are separated by 79 nucleotides
Shew_3015: Shew_3015 - gamma-glutamyl phosphate reductase (RefSeq), at 3,580,588 to 3,581,835
_3015
Shew_3016: Shew_3016 - pyrroline-5-carboxylate reductase (RefSeq), at 3,581,922 to 3,582,716
_3016
Shew_3017: Shew_3017 - glycerophosphoryl diester phosphodiesterase (RefSeq), at 3,582,796 to 3,584,091
_3017
Position (kb)
3581
3582
3583 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3581.060 kb on + strand, within Shew_3015 at 3581.060 kb on + strand, within Shew_3015 at 3581.068 kb on - strand, within Shew_3015 at 3581.171 kb on + strand, within Shew_3015 at 3581.253 kb on + strand, within Shew_3015 at 3581.261 kb on - strand, within Shew_3015 at 3581.261 kb on - strand, within Shew_3015 at 3581.298 kb on + strand, within Shew_3015 at 3581.357 kb on - strand, within Shew_3015 at 3581.424 kb on + strand, within Shew_3015 at 3581.663 kb on + strand, within Shew_3015 at 3581.984 kb on - strand at 3582.406 kb on + strand, within Shew_3016 at 3582.576 kb on + strand, within Shew_3016 at 3582.610 kb on + strand, within Shew_3016 at 3582.613 kb on - strand, within Shew_3016 at 3582.921 kb on + strand at 3583.257 kb on + strand, within Shew_3017 at 3583.382 kb on - strand, within Shew_3017 at 3583.400 kb on + strand, within Shew_3017 at 3583.508 kb on + strand, within Shew_3017 at 3583.631 kb on + strand, within Shew_3017
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,581,060 + Shew_3015 0.38 +0.5 3,581,060 + Shew_3015 0.38 +0.3 3,581,068 - Shew_3015 0.38 +0.7 3,581,171 + Shew_3015 0.47 -0.5 3,581,253 + Shew_3015 0.53 +0.2 3,581,261 - Shew_3015 0.54 -0.0 3,581,261 - Shew_3015 0.54 -0.2 3,581,298 + Shew_3015 0.57 -0.2 3,581,357 - Shew_3015 0.62 -2.8 3,581,424 + Shew_3015 0.67 +0.1 3,581,663 + Shew_3015 0.86 -0.6 3,581,984 - -0.2 3,582,406 + Shew_3016 0.61 +0.1 3,582,576 + Shew_3016 0.82 +0.3 3,582,610 + Shew_3016 0.87 +1.1 3,582,613 - Shew_3016 0.87 +0.4 3,582,921 + +0.2 3,583,257 + Shew_3017 0.36 -0.7 3,583,382 - Shew_3017 0.45 -0.2 3,583,400 + Shew_3017 0.47 +0.1 3,583,508 + Shew_3017 0.55 +1.5 3,583,631 + Shew_3017 0.64 -0.8
Or see this region's nucleotide sequence