Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2861 and Shew_2862 are separated by 4 nucleotides Shew_2862 and Shew_2863 are separated by 14 nucleotides Shew_2863 and Shew_2864 are separated by 32 nucleotides
Shew_2861: Shew_2861 - aminoglycoside phosphotransferase (RefSeq), at 3,392,700 to 3,393,737
_2861
Shew_2862: Shew_2862 - short chain dehydrogenase (RefSeq), at 3,393,742 to 3,394,509
_2862
Shew_2863: Shew_2863 - short-chain dehydrogenase/reductase SDR (RefSeq), at 3,394,524 to 3,395,270
_2863
Shew_2864: Shew_2864 - 3-hydroxyacyl-CoA dehydrogenase, NAD-binding (RefSeq), at 3,395,303 to 3,397,429
_2864
Position (kb)
3394
3395
3396 Strain fitness (log2 ratio)
-1
0
1 at 3393.974 kb on + strand, within Shew_2862 at 3394.038 kb on + strand, within Shew_2862 at 3394.598 kb on + strand at 3394.606 kb on - strand, within Shew_2863 at 3394.606 kb on - strand, within Shew_2863 at 3395.036 kb on - strand, within Shew_2863 at 3395.083 kb on + strand, within Shew_2863 at 3395.083 kb on + strand, within Shew_2863 at 3395.135 kb on + strand, within Shew_2863 at 3395.188 kb on - strand, within Shew_2863 at 3395.606 kb on - strand, within Shew_2864 at 3395.890 kb on + strand, within Shew_2864 at 3395.890 kb on + strand, within Shew_2864 at 3395.890 kb on + strand, within Shew_2864 at 3395.898 kb on - strand, within Shew_2864 at 3395.910 kb on + strand, within Shew_2864
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,393,974 + Shew_2862 0.30 +0.3 3,394,038 + Shew_2862 0.39 +0.3 3,394,598 + +0.9 3,394,606 - Shew_2863 0.11 +1.0 3,394,606 - Shew_2863 0.11 +1.4 3,395,036 - Shew_2863 0.69 -0.4 3,395,083 + Shew_2863 0.75 +0.2 3,395,083 + Shew_2863 0.75 +0.4 3,395,135 + Shew_2863 0.82 +0.3 3,395,188 - Shew_2863 0.89 +0.5 3,395,606 - Shew_2864 0.14 +0.7 3,395,890 + Shew_2864 0.28 +0.2 3,395,890 + Shew_2864 0.28 -0.0 3,395,890 + Shew_2864 0.28 +0.2 3,395,898 - Shew_2864 0.28 +0.3 3,395,910 + Shew_2864 0.29 +0.4
Or see this region's nucleotide sequence