Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2567 and Shew_2568 are separated by 384 nucleotides Shew_2568 and Shew_2569 are separated by 318 nucleotides Shew_2569 and Shew_2570 are separated by 111 nucleotides
Shew_2567: Shew_2567 - MORN repeat-containing protein (RefSeq), at 3,024,276 to 3,025,259
_2567
Shew_2568: Shew_2568 - AMP-dependent synthetase and ligase (RefSeq), at 3,025,644 to 3,027,632
_2568
Shew_2569: Shew_2569 - MerR family transcriptional regulator (RefSeq), at 3,027,951 to 3,028,352
_2569
Shew_2570: Shew_2570 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 3,028,464 to 3,029,633
_2570
Position (kb)
3025
3026
3027
3028 Strain fitness (log2 ratio)
-1
0
1
2 at 3024.937 kb on - strand, within Shew_2567 at 3024.952 kb on + strand, within Shew_2567 at 3025.491 kb on + strand at 3025.642 kb on + strand at 3025.668 kb on - strand at 3025.674 kb on + strand at 3025.682 kb on - strand at 3025.682 kb on - strand at 3025.682 kb on - strand at 3025.729 kb on + strand at 3025.784 kb on - strand at 3025.943 kb on - strand, within Shew_2568 at 3026.421 kb on + strand, within Shew_2568 at 3026.424 kb on - strand, within Shew_2568 at 3026.424 kb on - strand, within Shew_2568 at 3026.496 kb on - strand, within Shew_2568 at 3026.623 kb on + strand, within Shew_2568 at 3026.641 kb on - strand, within Shew_2568 at 3026.808 kb on - strand, within Shew_2568 at 3026.965 kb on - strand, within Shew_2568 at 3027.414 kb on + strand, within Shew_2568 at 3027.473 kb on - strand at 3028.211 kb on + strand, within Shew_2569 at 3028.520 kb on + strand at 3028.520 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 3,024,937 - Shew_2567 0.67 +0.3 3,024,952 + Shew_2567 0.69 -0.2 3,025,491 + +0.5 3,025,642 + +0.6 3,025,668 - +1.6 3,025,674 + +0.6 3,025,682 - +0.3 3,025,682 - -0.8 3,025,682 - +0.6 3,025,729 + +0.1 3,025,784 - +0.0 3,025,943 - Shew_2568 0.15 -0.2 3,026,421 + Shew_2568 0.39 -0.2 3,026,424 - Shew_2568 0.39 +0.7 3,026,424 - Shew_2568 0.39 +0.1 3,026,496 - Shew_2568 0.43 +0.4 3,026,623 + Shew_2568 0.49 +0.6 3,026,641 - Shew_2568 0.50 +0.1 3,026,808 - Shew_2568 0.59 +0.2 3,026,965 - Shew_2568 0.66 +0.7 3,027,414 + Shew_2568 0.89 +2.5 3,027,473 - +0.3 3,028,211 + Shew_2569 0.65 +0.7 3,028,520 + +0.9 3,028,520 + +0.7
Or see this region's nucleotide sequence