Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2245 and Shew_2246 are separated by 302 nucleotides Shew_2246 and Shew_2247 overlap by 23 nucleotides Shew_2247 and Shew_2248 are separated by 0 nucleotides
Shew_2245: Shew_2245 - hypothetical protein (RefSeq), at 2,624,567 to 2,625,481
_2245
Shew_2246: Shew_2246 - pseudouridine synthase (RefSeq), at 2,625,784 to 2,626,665
_2246
Shew_2247: Shew_2247 - putative transcriptional regulator (RefSeq), at 2,626,643 to 2,627,275
_2247
Shew_2248: Shew_2248 - chromosome segregation and condensation protein ScpA (RefSeq), at 2,627,276 to 2,628,064
_2248
Position (kb)
2625
2626
2627 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2625.024 kb on + strand, within Shew_2245 at 2625.024 kb on + strand, within Shew_2245 at 2625.024 kb on + strand, within Shew_2245 at 2625.032 kb on - strand, within Shew_2245 at 2625.032 kb on - strand, within Shew_2245 at 2625.032 kb on - strand, within Shew_2245 at 2625.032 kb on - strand, within Shew_2245 at 2625.192 kb on + strand, within Shew_2245 at 2625.888 kb on - strand, within Shew_2246 at 2625.949 kb on - strand, within Shew_2246 at 2626.420 kb on + strand, within Shew_2246 at 2626.629 kb on - strand at 2627.504 kb on - strand, within Shew_2248
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,625,024 + Shew_2245 0.50 +0.5 2,625,024 + Shew_2245 0.50 -0.7 2,625,024 + Shew_2245 0.50 +0.7 2,625,032 - Shew_2245 0.51 +0.2 2,625,032 - Shew_2245 0.51 -0.9 2,625,032 - Shew_2245 0.51 +0.6 2,625,032 - Shew_2245 0.51 -0.1 2,625,192 + Shew_2245 0.68 -0.3 2,625,888 - Shew_2246 0.12 -0.8 2,625,949 - Shew_2246 0.19 -3.0 2,626,420 + Shew_2246 0.72 -2.4 2,626,629 - -3.1 2,627,504 - Shew_2248 0.29 +0.1
Or see this region's nucleotide sequence