Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddl and Shew_2134 are separated by 94 nucleotides Shew_2134 and ushA are separated by 400 nucleotides ushA and Shew_2136 are separated by 604 nucleotides
Shew_2133: ddl - D-alanyl-alanine synthetase A (RefSeq), at 2,479,110 to 2,480,114
ddl
Shew_2134: Shew_2134 - heat shock protein Hsp20 (RefSeq), at 2,480,209 to 2,480,646
_2134
Shew_2135: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,481,047 to 2,482,750
ushA
Shew_2136: Shew_2136 - carboxylesterase (RefSeq), at 2,483,355 to 2,484,029
_2136
Position (kb)
2481
2482
2483 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2480.523 kb on + strand, within Shew_2134 at 2480.531 kb on - strand, within Shew_2134 at 2480.594 kb on - strand, within Shew_2134 at 2480.655 kb on - strand at 2480.760 kb on - strand at 2480.809 kb on - strand at 2480.907 kb on - strand at 2480.951 kb on - strand at 2480.974 kb on + strand at 2480.974 kb on + strand at 2481.076 kb on - strand at 2481.347 kb on - strand, within ushA at 2481.537 kb on + strand, within ushA at 2481.911 kb on - strand, within ushA at 2482.444 kb on + strand, within ushA at 2482.452 kb on - strand, within ushA at 2483.225 kb on - strand at 2483.506 kb on + strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.708 kb on + strand, within Shew_2136 at 2483.716 kb on - strand, within Shew_2136
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,480,523 + Shew_2134 0.72 -2.3 2,480,531 - Shew_2134 0.74 +0.0 2,480,594 - Shew_2134 0.88 +0.2 2,480,655 - -0.2 2,480,760 - +0.7 2,480,809 - +0.0 2,480,907 - -0.5 2,480,951 - +1.3 2,480,974 + +0.8 2,480,974 + -0.3 2,481,076 - +0.5 2,481,347 - ushA Shew_2135 0.18 +1.7 2,481,537 + ushA Shew_2135 0.29 +0.0 2,481,911 - ushA Shew_2135 0.51 -0.9 2,482,444 + ushA Shew_2135 0.82 +0.9 2,482,452 - ushA Shew_2135 0.82 +1.9 2,483,225 - +0.8 2,483,506 + Shew_2136 0.22 -0.0 2,483,514 - Shew_2136 0.24 +0.0 2,483,514 - Shew_2136 0.24 +0.0 2,483,708 + Shew_2136 0.52 +0.4 2,483,716 - Shew_2136 0.53 +0.8
Or see this region's nucleotide sequence