Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2115 and Shew_2116 are separated by 217 nucleotides Shew_2116 and Shew_2117 are separated by 37 nucleotides Shew_2117 and Shew_2118 are separated by 209 nucleotides
Shew_2115: Shew_2115 - lytic transglycosylase, catalytic (RefSeq), at 2,450,005 to 2,451,540
_2115
Shew_2116: Shew_2116 - AsmA family protein (RefSeq), at 2,451,758 to 2,453,602
_2116
Shew_2117: Shew_2117 - ribonuclease activity regulator protein RraA (RefSeq), at 2,453,640 to 2,454,134
_2117
Shew_2118: Shew_2118 - hypothetical protein (RefSeq), at 2,454,344 to 2,454,658
_2118
Position (kb)
2451
2452
2453
2454 Strain fitness (log2 ratio)
-2
-1
0
1 at 2450.780 kb on - strand, within Shew_2115 at 2451.108 kb on - strand, within Shew_2115 at 2451.376 kb on - strand, within Shew_2115 at 2451.560 kb on + strand at 2452.011 kb on - strand, within Shew_2116 at 2452.092 kb on - strand, within Shew_2116 at 2452.440 kb on + strand, within Shew_2116 at 2452.758 kb on + strand, within Shew_2116 at 2453.209 kb on - strand, within Shew_2116 at 2453.280 kb on + strand, within Shew_2116 at 2453.388 kb on - strand, within Shew_2116 at 2453.434 kb on - strand at 2453.529 kb on - strand at 2453.910 kb on + strand, within Shew_2117 at 2454.153 kb on - strand at 2454.474 kb on + strand, within Shew_2118 at 2454.533 kb on + strand, within Shew_2118 at 2454.571 kb on - strand, within Shew_2118
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 2,450,780 - Shew_2115 0.50 +0.0 2,451,108 - Shew_2115 0.72 -0.1 2,451,376 - Shew_2115 0.89 +0.9 2,451,560 + +0.5 2,452,011 - Shew_2116 0.14 -2.7 2,452,092 - Shew_2116 0.18 +0.1 2,452,440 + Shew_2116 0.37 -1.9 2,452,758 + Shew_2116 0.54 +0.3 2,453,209 - Shew_2116 0.79 -1.2 2,453,280 + Shew_2116 0.82 -0.4 2,453,388 - Shew_2116 0.88 -0.3 2,453,434 - -0.8 2,453,529 - -1.7 2,453,910 + Shew_2117 0.55 -1.4 2,454,153 - +0.5 2,454,474 + Shew_2118 0.41 +0.9 2,454,533 + Shew_2118 0.60 +0.2 2,454,571 - Shew_2118 0.72 +0.3
Or see this region's nucleotide sequence