Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1401 and Shew_1402 overlap by 1 nucleotides Shew_1402 and Shew_1403 are separated by 9 nucleotides Shew_1403 and Shew_1404 are separated by 2 nucleotides
Shew_1401: Shew_1401 - dTDP-glucose 4,6 dehydratase (RefSeq), at 1,613,792 to 1,614,889
_1401
Shew_1402: Shew_1402 - glucose-1-phosphate thymidylyltransferase (RefSeq), at 1,614,889 to 1,615,764
_1402
Shew_1403: Shew_1403 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 1,615,774 to 1,616,640
_1403
Shew_1404: Shew_1404 - dTDP-4-dehydrorhamnose 3,5-epimerase (RefSeq), at 1,616,643 to 1,617,188
_1404
Position (kb)
1614
1615
1616 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1614.020 kb on + strand, within Shew_1401 at 1614.020 kb on + strand, within Shew_1401 at 1614.040 kb on + strand, within Shew_1401 at 1614.040 kb on + strand, within Shew_1401 at 1614.102 kb on - strand, within Shew_1401 at 1614.110 kb on - strand, within Shew_1401 at 1614.196 kb on + strand, within Shew_1401 at 1614.340 kb on + strand, within Shew_1401 at 1614.342 kb on + strand, within Shew_1401 at 1614.342 kb on + strand, within Shew_1401 at 1614.342 kb on + strand, within Shew_1401 at 1614.342 kb on + strand, within Shew_1401 at 1614.448 kb on + strand, within Shew_1401 at 1614.448 kb on + strand, within Shew_1401 at 1614.456 kb on - strand, within Shew_1401 at 1614.466 kb on - strand, within Shew_1401 at 1614.466 kb on - strand, within Shew_1401 at 1614.520 kb on + strand, within Shew_1401 at 1614.520 kb on + strand, within Shew_1401 at 1614.520 kb on + strand, within Shew_1401 at 1614.612 kb on + strand, within Shew_1401 at 1614.686 kb on + strand, within Shew_1401 at 1614.731 kb on + strand, within Shew_1401 at 1614.787 kb on - strand at 1614.787 kb on + strand at 1614.787 kb on + strand at 1614.795 kb on - strand at 1614.811 kb on + strand at 1614.848 kb on + strand at 1614.933 kb on - strand at 1615.134 kb on + strand, within Shew_1402 at 1615.142 kb on + strand, within Shew_1402 at 1615.147 kb on + strand, within Shew_1402 at 1615.147 kb on + strand, within Shew_1402 at 1615.153 kb on + strand, within Shew_1402 at 1615.296 kb on + strand, within Shew_1402 at 1615.327 kb on - strand, within Shew_1402 at 1615.500 kb on + strand, within Shew_1402 at 1615.557 kb on + strand, within Shew_1402 at 1615.559 kb on + strand, within Shew_1402 at 1615.604 kb on + strand, within Shew_1402 at 1615.604 kb on + strand, within Shew_1402 at 1615.622 kb on + strand, within Shew_1402 at 1615.723 kb on + strand at 1615.780 kb on + strand at 1615.800 kb on - strand at 1615.853 kb on + strand at 1615.862 kb on + strand, within Shew_1403 at 1616.035 kb on + strand, within Shew_1403 at 1616.130 kb on + strand, within Shew_1403 at 1616.137 kb on + strand, within Shew_1403 at 1616.137 kb on + strand, within Shew_1403 at 1616.137 kb on + strand, within Shew_1403 at 1616.167 kb on + strand, within Shew_1403 at 1616.314 kb on + strand, within Shew_1403 at 1616.344 kb on + strand, within Shew_1403 at 1616.410 kb on + strand, within Shew_1403 at 1616.410 kb on + strand, within Shew_1403 at 1616.410 kb on + strand, within Shew_1403 at 1616.410 kb on + strand, within Shew_1403 at 1616.418 kb on - strand, within Shew_1403 at 1616.488 kb on + strand, within Shew_1403 at 1616.606 kb on + strand at 1616.614 kb on - strand at 1616.753 kb on + strand, within Shew_1404 at 1616.753 kb on + strand, within Shew_1404 at 1616.753 kb on + strand, within Shew_1404 at 1616.753 kb on + strand, within Shew_1404 at 1616.753 kb on + strand, within Shew_1404
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,614,020 + Shew_1401 0.21 -4.0 1,614,020 + Shew_1401 0.21 -3.2 1,614,040 + Shew_1401 0.23 -3.8 1,614,040 + Shew_1401 0.23 -2.9 1,614,102 - Shew_1401 0.28 -3.5 1,614,110 - Shew_1401 0.29 -0.5 1,614,196 + Shew_1401 0.37 -3.1 1,614,340 + Shew_1401 0.50 -2.8 1,614,342 + Shew_1401 0.50 -3.5 1,614,342 + Shew_1401 0.50 -2.9 1,614,342 + Shew_1401 0.50 -2.9 1,614,342 + Shew_1401 0.50 -3.7 1,614,448 + Shew_1401 0.60 -2.4 1,614,448 + Shew_1401 0.60 -3.0 1,614,456 - Shew_1401 0.60 -2.1 1,614,466 - Shew_1401 0.61 -1.1 1,614,466 - Shew_1401 0.61 +0.5 1,614,520 + Shew_1401 0.66 -3.0 1,614,520 + Shew_1401 0.66 -1.9 1,614,520 + Shew_1401 0.66 -4.1 1,614,612 + Shew_1401 0.75 -3.2 1,614,686 + Shew_1401 0.81 -3.2 1,614,731 + Shew_1401 0.86 -4.4 1,614,787 - -0.5 1,614,787 + -1.1 1,614,787 + +0.7 1,614,795 - -0.1 1,614,811 + +0.6 1,614,848 + -1.8 1,614,933 - -1.1 1,615,134 + Shew_1402 0.28 -3.9 1,615,142 + Shew_1402 0.29 +0.9 1,615,147 + Shew_1402 0.29 -2.7 1,615,147 + Shew_1402 0.29 -2.1 1,615,153 + Shew_1402 0.30 -4.1 1,615,296 + Shew_1402 0.46 -3.5 1,615,327 - Shew_1402 0.50 -0.5 1,615,500 + Shew_1402 0.70 -5.1 1,615,557 + Shew_1402 0.76 -4.2 1,615,559 + Shew_1402 0.76 -3.3 1,615,604 + Shew_1402 0.82 -2.0 1,615,604 + Shew_1402 0.82 -2.2 1,615,622 + Shew_1402 0.84 -2.6 1,615,723 + -4.2 1,615,780 + -3.0 1,615,800 - -1.5 1,615,853 + -3.0 1,615,862 + Shew_1403 0.10 -2.3 1,616,035 + Shew_1403 0.30 -3.6 1,616,130 + Shew_1403 0.41 -2.4 1,616,137 + Shew_1403 0.42 -3.0 1,616,137 + Shew_1403 0.42 -4.3 1,616,137 + Shew_1403 0.42 -3.5 1,616,167 + Shew_1403 0.45 -2.0 1,616,314 + Shew_1403 0.62 -3.0 1,616,344 + Shew_1403 0.66 -2.3 1,616,410 + Shew_1403 0.73 -3.9 1,616,410 + Shew_1403 0.73 -2.5 1,616,410 + Shew_1403 0.73 -2.3 1,616,410 + Shew_1403 0.73 -4.0 1,616,418 - Shew_1403 0.74 +0.5 1,616,488 + Shew_1403 0.82 -2.7 1,616,606 + -3.5 1,616,614 - +0.5 1,616,753 + Shew_1404 0.20 -3.1 1,616,753 + Shew_1404 0.20 -2.5 1,616,753 + Shew_1404 0.20 -4.2 1,616,753 + Shew_1404 0.20 -3.2 1,616,753 + Shew_1404 0.20 -3.0
Or see this region's nucleotide sequence