Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1224 and Shew_1225 are separated by 277 nucleotides Shew_1225 and Shew_1226 are separated by 85 nucleotides Shew_1226 and Shew_1227 are separated by 198 nucleotides
Shew_1224: Shew_1224 - sodium:dicarboxylate symporter (RefSeq), at 1,421,398 to 1,422,648
_1224
Shew_1225: Shew_1225 - hypothetical protein (RefSeq), at 1,422,926 to 1,423,348
_1225
Shew_1226: Shew_1226 - peptidase M16 domain-containing protein (RefSeq), at 1,423,434 to 1,426,268
_1226
Shew_1227: Shew_1227 - glutamate--cysteine ligase (RefSeq), at 1,426,467 to 1,428,038
_1227
Position (kb)
1423
1424
1425
1426
1427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1422.720 kb on + strand at 1423.070 kb on + strand, within Shew_1225 at 1423.089 kb on + strand, within Shew_1225 at 1423.251 kb on + strand, within Shew_1225 at 1423.400 kb on + strand at 1423.400 kb on + strand at 1423.408 kb on - strand at 1423.732 kb on + strand, within Shew_1226 at 1423.740 kb on - strand, within Shew_1226 at 1424.128 kb on + strand, within Shew_1226 at 1424.201 kb on + strand, within Shew_1226 at 1424.251 kb on - strand, within Shew_1226 at 1424.474 kb on + strand, within Shew_1226 at 1424.596 kb on + strand, within Shew_1226 at 1424.903 kb on + strand, within Shew_1226 at 1424.986 kb on - strand, within Shew_1226 at 1425.360 kb on - strand, within Shew_1226 at 1425.438 kb on - strand, within Shew_1226 at 1425.438 kb on - strand, within Shew_1226 at 1425.438 kb on - strand, within Shew_1226 at 1425.544 kb on + strand, within Shew_1226 at 1425.562 kb on - strand, within Shew_1226 at 1425.677 kb on + strand, within Shew_1226 at 1425.766 kb on + strand, within Shew_1226 at 1425.824 kb on + strand, within Shew_1226 at 1425.886 kb on - strand, within Shew_1226 at 1425.944 kb on + strand, within Shew_1226 at 1426.008 kb on - strand at 1426.153 kb on - strand at 1426.241 kb on - strand at 1426.602 kb on + strand at 1426.706 kb on + strand, within Shew_1227
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 1,422,720 + +3.2 1,423,070 + Shew_1225 0.34 -0.8 1,423,089 + Shew_1225 0.39 -2.2 1,423,251 + Shew_1225 0.77 -0.7 1,423,400 + +0.9 1,423,400 + +0.8 1,423,408 - -1.6 1,423,732 + Shew_1226 0.11 -0.5 1,423,740 - Shew_1226 0.11 -1.3 1,424,128 + Shew_1226 0.24 -0.5 1,424,201 + Shew_1226 0.27 -0.9 1,424,251 - Shew_1226 0.29 -1.3 1,424,474 + Shew_1226 0.37 -1.6 1,424,596 + Shew_1226 0.41 -0.4 1,424,903 + Shew_1226 0.52 -0.7 1,424,986 - Shew_1226 0.55 -0.1 1,425,360 - Shew_1226 0.68 -0.9 1,425,438 - Shew_1226 0.71 -1.2 1,425,438 - Shew_1226 0.71 -0.1 1,425,438 - Shew_1226 0.71 -3.0 1,425,544 + Shew_1226 0.74 -0.7 1,425,562 - Shew_1226 0.75 -2.5 1,425,677 + Shew_1226 0.79 -1.7 1,425,766 + Shew_1226 0.82 -1.7 1,425,824 + Shew_1226 0.84 -0.2 1,425,886 - Shew_1226 0.86 -0.6 1,425,944 + Shew_1226 0.89 -1.8 1,426,008 - -1.0 1,426,153 - -0.9 1,426,241 - -0.8 1,426,602 + +2.1 1,426,706 + Shew_1227 0.15 +1.6
Or see this region's nucleotide sequence