Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0815 and Shew_0816 are separated by 263 nucleotides Shew_0816 and Shew_0817 are separated by 11 nucleotides Shew_0817 and Shew_0818 are separated by 167 nucleotides
Shew_0815: Shew_0815 - Glucosamine-6-phosphate deaminase (EC 3.5.99.6) <small>(from data)</small>, at 939,182 to 939,988
_0815
Shew_0816: Shew_0816 - hypothetical protein (RefSeq), at 940,252 to 940,788
_0816
Shew_0817: Shew_0817 - cupin 2 domain-containing protein (RefSeq), at 940,800 to 941,117
_0817
Shew_0818: Shew_0818 - peptidase U32 (RefSeq), at 941,285 to 943,198
_0818
Position (kb) 940
941 Strain fitness (log2 ratio) -1
0
1
2 at 939.369 kb on + strand, within Shew_0815 at 939.615 kb on + strand, within Shew_0815 at 939.623 kb on - strand, within Shew_0815 at 939.787 kb on + strand, within Shew_0815 at 939.789 kb on - strand, within Shew_0815 at 939.809 kb on + strand, within Shew_0815 at 940.489 kb on - strand, within Shew_0816 at 940.722 kb on + strand, within Shew_0816 at 940.773 kb on + strand at 941.015 kb on - strand, within Shew_0817 at 941.637 kb on - strand, within Shew_0818 at 941.743 kb on + strand, within Shew_0818 at 941.762 kb on - strand, within Shew_0818
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 939,369 + Shew_0815 0.23 +0.3 939,615 + Shew_0815 0.54 +0.1 939,623 - Shew_0815 0.55 +1.2 939,787 + Shew_0815 0.75 -0.5 939,789 - Shew_0815 0.75 +0.4 939,809 + Shew_0815 0.78 -0.1 940,489 - Shew_0816 0.44 +0.5 940,722 + Shew_0816 0.88 +0.4 940,773 + +2.2 941,015 - Shew_0817 0.68 +0.3 941,637 - Shew_0818 0.18 -0.2 941,743 + Shew_0818 0.24 +1.2 941,762 - Shew_0818 0.25 +0.6
Or see this region's nucleotide sequence