Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0499 and Shew_0500 are separated by 88 nucleotides Shew_0500 and Shew_0501 are separated by 202 nucleotides Shew_0501 and Shew_0502 are separated by 9 nucleotides
Shew_0499: Shew_0499 - Xaa-Pro aminopeptidase family enzyme (RefSeq), at 557,784 to 559,136
_0499
Shew_0500: Shew_0500 - hypothetical protein (RefSeq), at 559,225 to 560,241
_0500
Shew_0501: Shew_0501 - hypothetical protein (RefSeq), at 560,444 to 560,728
_0501
Shew_0502: Shew_0502 - hypothetical protein (RefSeq), at 560,738 to 561,265
_0502
Position (kb) 559
560
561 Strain fitness (log2 ratio) -1
0
1 at 558.375 kb on - strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.411 kb on - strand, within Shew_0499 at 558.608 kb on + strand, within Shew_0499 at 558.679 kb on + strand, within Shew_0499 at 559.472 kb on + strand, within Shew_0500 at 559.980 kb on - strand, within Shew_0500 at 560.254 kb on + strand at 560.257 kb on - strand at 560.272 kb on - strand at 560.294 kb on + strand at 560.487 kb on + strand, within Shew_0501 at 560.497 kb on + strand, within Shew_0501 at 560.699 kb on + strand, within Shew_0501 at 560.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 558,375 - Shew_0499 0.44 -0.4 558,403 + Shew_0499 0.46 +0.4 558,403 + Shew_0499 0.46 +1.1 558,403 + Shew_0499 0.46 +0.7 558,411 - Shew_0499 0.46 +0.2 558,608 + Shew_0499 0.61 +0.8 558,679 + Shew_0499 0.66 -1.5 559,472 + Shew_0500 0.24 +0.4 559,980 - Shew_0500 0.74 -0.0 560,254 + +1.5 560,257 - +0.2 560,272 - +1.3 560,294 + +0.3 560,487 + Shew_0501 0.15 +0.2 560,497 + Shew_0501 0.19 +0.1 560,699 + Shew_0501 0.89 -1.4 560,707 - -0.4
Or see this region's nucleotide sequence