Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0497 and Shew_0498 are separated by 5 nucleotides Shew_0498 and Shew_0499 are separated by 145 nucleotides Shew_0499 and Shew_0500 are separated by 88 nucleotides
Shew_0497: Shew_0497 - putative sulfate transporter YchM (RefSeq), at 555,306 to 557,003
_0497
Shew_0498: Shew_0498 - flavoprotein oxygenase (RefSeq), at 557,009 to 557,638
_0498
Shew_0499: Shew_0499 - Xaa-Pro aminopeptidase family enzyme (RefSeq), at 557,784 to 559,136
_0499
Shew_0500: Shew_0500 - hypothetical protein (RefSeq), at 559,225 to 560,241
_0500
Position (kb) 557
558
559
560 Strain fitness (log2 ratio) -1
0
1 at 557.090 kb on - strand, within Shew_0498 at 557.102 kb on - strand, within Shew_0498 at 557.170 kb on + strand, within Shew_0498 at 557.178 kb on - strand, within Shew_0498 at 557.453 kb on + strand, within Shew_0498 at 557.885 kb on - strand at 558.001 kb on + strand, within Shew_0499 at 558.375 kb on - strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.403 kb on + strand, within Shew_0499 at 558.411 kb on - strand, within Shew_0499 at 558.608 kb on + strand, within Shew_0499 at 558.679 kb on + strand, within Shew_0499 at 559.472 kb on + strand, within Shew_0500 at 559.980 kb on - strand, within Shew_0500
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 557,090 - Shew_0498 0.13 +0.4 557,102 - Shew_0498 0.15 +0.5 557,170 + Shew_0498 0.26 +0.2 557,178 - Shew_0498 0.27 +1.5 557,453 + Shew_0498 0.70 +0.8 557,885 - -0.8 558,001 + Shew_0499 0.16 +0.4 558,375 - Shew_0499 0.44 -0.4 558,403 + Shew_0499 0.46 +0.4 558,403 + Shew_0499 0.46 +1.1 558,403 + Shew_0499 0.46 +0.7 558,411 - Shew_0499 0.46 +0.2 558,608 + Shew_0499 0.61 +0.8 558,679 + Shew_0499 0.66 -1.5 559,472 + Shew_0500 0.24 +0.4 559,980 - Shew_0500 0.74 -0.0
Or see this region's nucleotide sequence