Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0413 and Shew_0414 overlap by 4 nucleotides Shew_0414 and Shew_0415 are separated by 36 nucleotides Shew_0415 and Shew_0416 are separated by 146 nucleotides
Shew_0413: Shew_0413 - ABC-2 type transporter (RefSeq), at 466,858 to 468,030
_0413
Shew_0414: Shew_0414 - ABC-2 type transporter (RefSeq), at 468,027 to 469,172
_0414
Shew_0415: Shew_0415 - hypothetical protein (RefSeq), at 469,209 to 469,751
_0415
Shew_0416: Shew_0416 - peptidase U62, modulator of DNA gyrase (RefSeq), at 469,898 to 471,238
_0416
Position (kb) 468
469
470 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 467.115 kb on + strand, within Shew_0413 at 467.381 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.542 kb on + strand, within Shew_0413 at 467.686 kb on + strand, within Shew_0413 at 467.994 kb on - strand at 468.064 kb on - strand at 468.193 kb on + strand, within Shew_0414 at 468.303 kb on - strand, within Shew_0414 at 468.426 kb on + strand, within Shew_0414 at 468.427 kb on + strand, within Shew_0414 at 468.620 kb on - strand, within Shew_0414 at 468.721 kb on + strand, within Shew_0414 at 468.786 kb on - strand, within Shew_0414 at 468.852 kb on - strand, within Shew_0414 at 469.364 kb on - strand, within Shew_0415 at 469.515 kb on - strand, within Shew_0415 at 469.560 kb on - strand, within Shew_0415 at 469.715 kb on + strand at 469.724 kb on + strand at 469.732 kb on - strand at 470.142 kb on - strand, within Shew_0416
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 467,115 + Shew_0413 0.22 -2.1 467,381 - Shew_0413 0.45 -1.1 467,438 - Shew_0413 0.49 -1.3 467,438 - Shew_0413 0.49 -0.7 467,542 + Shew_0413 0.58 +0.5 467,686 + Shew_0413 0.71 -3.2 467,994 - -2.2 468,064 - -2.3 468,193 + Shew_0414 0.14 -1.9 468,303 - Shew_0414 0.24 -1.8 468,426 + Shew_0414 0.35 -2.5 468,427 + Shew_0414 0.35 -1.5 468,620 - Shew_0414 0.52 -0.1 468,721 + Shew_0414 0.61 -2.1 468,786 - Shew_0414 0.66 -3.4 468,852 - Shew_0414 0.72 -1.6 469,364 - Shew_0415 0.29 -0.2 469,515 - Shew_0415 0.56 -0.1 469,560 - Shew_0415 0.65 +0.1 469,715 + -1.7 469,724 + -0.6 469,732 - +0.6 470,142 - Shew_0416 0.18 +0.1
Or see this region's nucleotide sequence