Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0411 and Shew_0412 overlap by 11 nucleotides Shew_0412 and Shew_0413 are separated by 12 nucleotides Shew_0413 and Shew_0414 overlap by 4 nucleotides
Shew_0411: Shew_0411 - outer membrane efflux protein (RefSeq), at 464,481 to 465,884
_0411
Shew_0412: Shew_0412 - secretion protein HlyD family protein (RefSeq), at 465,874 to 466,845
_0412
Shew_0413: Shew_0413 - ABC-2 type transporter (RefSeq), at 466,858 to 468,030
_0413
Shew_0414: Shew_0414 - ABC-2 type transporter (RefSeq), at 468,027 to 469,172
_0414
Position (kb) 466
467
468
469 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 465.858 kb on + strand at 466.097 kb on + strand, within Shew_0412 at 466.499 kb on + strand, within Shew_0412 at 466.519 kb on + strand, within Shew_0412 at 466.606 kb on - strand, within Shew_0412 at 466.636 kb on - strand, within Shew_0412 at 466.827 kb on - strand at 466.861 kb on + strand at 466.983 kb on - strand, within Shew_0413 at 467.115 kb on + strand, within Shew_0413 at 467.381 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.438 kb on - strand, within Shew_0413 at 467.542 kb on + strand, within Shew_0413 at 467.686 kb on + strand, within Shew_0413 at 467.994 kb on - strand at 468.064 kb on - strand at 468.193 kb on + strand, within Shew_0414 at 468.303 kb on - strand, within Shew_0414 at 468.426 kb on + strand, within Shew_0414 at 468.427 kb on + strand, within Shew_0414 at 468.620 kb on - strand, within Shew_0414 at 468.721 kb on + strand, within Shew_0414 at 468.786 kb on - strand, within Shew_0414 at 468.852 kb on - strand, within Shew_0414
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 465,858 + -0.9 466,097 + Shew_0412 0.23 -2.3 466,499 + Shew_0412 0.64 -2.0 466,519 + Shew_0412 0.66 -2.0 466,606 - Shew_0412 0.75 -3.1 466,636 - Shew_0412 0.78 -1.5 466,827 - -3.4 466,861 + -1.5 466,983 - Shew_0413 0.11 -0.9 467,115 + Shew_0413 0.22 -2.1 467,381 - Shew_0413 0.45 -1.1 467,438 - Shew_0413 0.49 -1.3 467,438 - Shew_0413 0.49 -0.7 467,542 + Shew_0413 0.58 +0.5 467,686 + Shew_0413 0.71 -3.2 467,994 - -2.2 468,064 - -2.3 468,193 + Shew_0414 0.14 -1.9 468,303 - Shew_0414 0.24 -1.8 468,426 + Shew_0414 0.35 -2.5 468,427 + Shew_0414 0.35 -1.5 468,620 - Shew_0414 0.52 -0.1 468,721 + Shew_0414 0.61 -2.1 468,786 - Shew_0414 0.66 -3.4 468,852 - Shew_0414 0.72 -1.6
Or see this region's nucleotide sequence