Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0410 and Shew_0411 are separated by 192 nucleotides Shew_0411 and Shew_0412 overlap by 11 nucleotides Shew_0412 and Shew_0413 are separated by 12 nucleotides
Shew_0410: Shew_0410 - peptidase U62, modulator of DNA gyrase (RefSeq), at 462,840 to 464,288
_0410
Shew_0411: Shew_0411 - outer membrane efflux protein (RefSeq), at 464,481 to 465,884
_0411
Shew_0412: Shew_0412 - secretion protein HlyD family protein (RefSeq), at 465,874 to 466,845
_0412
Shew_0413: Shew_0413 - ABC-2 type transporter (RefSeq), at 466,858 to 468,030
_0413
Position (kb) 464
465
466 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 463.869 kb on + strand, within Shew_0410 at 463.882 kb on - strand, within Shew_0410 at 463.940 kb on - strand, within Shew_0410 at 464.291 kb on - strand at 464.626 kb on - strand, within Shew_0411 at 464.665 kb on - strand, within Shew_0411 at 464.937 kb on + strand, within Shew_0411 at 465.036 kb on - strand, within Shew_0411 at 465.243 kb on - strand, within Shew_0411 at 465.268 kb on + strand, within Shew_0411 at 465.276 kb on - strand, within Shew_0411 at 465.557 kb on + strand, within Shew_0411 at 465.797 kb on - strand at 465.797 kb on - strand at 465.797 kb on - strand at 465.858 kb on + strand at 466.097 kb on + strand, within Shew_0412 at 466.499 kb on + strand, within Shew_0412 at 466.519 kb on + strand, within Shew_0412 at 466.606 kb on - strand, within Shew_0412 at 466.636 kb on - strand, within Shew_0412 at 466.827 kb on - strand at 466.861 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 463,869 + Shew_0410 0.71 +0.6 463,882 - Shew_0410 0.72 +0.4 463,940 - Shew_0410 0.76 -0.4 464,291 - -0.2 464,626 - Shew_0411 0.10 -2.9 464,665 - Shew_0411 0.13 -1.6 464,937 + Shew_0411 0.32 +0.7 465,036 - Shew_0411 0.40 -1.6 465,243 - Shew_0411 0.54 -2.7 465,268 + Shew_0411 0.56 -2.1 465,276 - Shew_0411 0.57 -2.4 465,557 + Shew_0411 0.77 +0.4 465,797 - -1.3 465,797 - -1.7 465,797 - -2.6 465,858 + -0.9 466,097 + Shew_0412 0.23 -2.3 466,499 + Shew_0412 0.64 -2.0 466,519 + Shew_0412 0.66 -2.0 466,606 - Shew_0412 0.75 -3.1 466,636 - Shew_0412 0.78 -1.5 466,827 - -3.4 466,861 + -1.5
Or see this region's nucleotide sequence