Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0407 and Shew_0408 overlap by 1 nucleotides Shew_0408 and Shew_0409 overlap by 77 nucleotides Shew_0409 and Shew_0410 are separated by 41 nucleotides
Shew_0407: Shew_0407 - ribonuclease G (RefSeq), at 456,232 to 457,734
_0407
Shew_0408: Shew_0408 - hypothetical protein (RefSeq), at 457,734 to 462,044
_0408
Shew_0409: Shew_0409 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (RefSeq), at 461,968 to 462,798
_0409
Shew_0410: Shew_0410 - peptidase U62, modulator of DNA gyrase (RefSeq), at 462,840 to 464,288
_0410
Position (kb) 457
458
459
460
461
462
463 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 456.840 kb on + strand, within Shew_0407 at 457.184 kb on - strand, within Shew_0407 at 457.203 kb on + strand, within Shew_0407 at 457.219 kb on + strand, within Shew_0407 at 457.834 kb on - strand at 457.834 kb on - strand at 458.047 kb on + strand at 458.232 kb on + strand, within Shew_0408 at 458.427 kb on + strand, within Shew_0408 at 458.427 kb on + strand, within Shew_0408 at 458.548 kb on - strand, within Shew_0408 at 458.732 kb on - strand, within Shew_0408 at 458.898 kb on - strand, within Shew_0408 at 458.898 kb on - strand, within Shew_0408 at 458.898 kb on - strand, within Shew_0408 at 458.937 kb on - strand, within Shew_0408 at 459.157 kb on + strand, within Shew_0408 at 459.289 kb on - strand, within Shew_0408 at 459.678 kb on + strand, within Shew_0408 at 459.733 kb on - strand, within Shew_0408 at 459.819 kb on + strand, within Shew_0408 at 460.268 kb on + strand, within Shew_0408 at 460.344 kb on - strand, within Shew_0408 at 460.474 kb on - strand, within Shew_0408 at 460.791 kb on - strand, within Shew_0408 at 460.958 kb on + strand, within Shew_0408 at 460.966 kb on - strand, within Shew_0408 at 461.314 kb on + strand, within Shew_0408 at 461.322 kb on - strand, within Shew_0408 at 461.322 kb on - strand, within Shew_0408 at 461.332 kb on - strand, within Shew_0408 at 461.345 kb on - strand, within Shew_0408 at 461.576 kb on + strand, within Shew_0408 at 461.685 kb on + strand at 461.769 kb on + strand at 462.097 kb on - strand, within Shew_0409 at 462.391 kb on - strand, within Shew_0409 at 462.431 kb on + strand, within Shew_0409 at 462.446 kb on - strand, within Shew_0409 at 462.615 kb on + strand, within Shew_0409 at 462.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 456,840 + Shew_0407 0.40 -4.3 457,184 - Shew_0407 0.63 -4.1 457,203 + Shew_0407 0.65 -2.8 457,219 + Shew_0407 0.66 -3.5 457,834 - -2.5 457,834 - -2.6 458,047 + -2.8 458,232 + Shew_0408 0.12 -2.8 458,427 + Shew_0408 0.16 -1.6 458,427 + Shew_0408 0.16 -1.0 458,548 - Shew_0408 0.19 -1.7 458,732 - Shew_0408 0.23 -2.4 458,898 - Shew_0408 0.27 -3.2 458,898 - Shew_0408 0.27 -2.0 458,898 - Shew_0408 0.27 -3.0 458,937 - Shew_0408 0.28 -2.0 459,157 + Shew_0408 0.33 -2.6 459,289 - Shew_0408 0.36 -2.1 459,678 + Shew_0408 0.45 -1.5 459,733 - Shew_0408 0.46 -2.6 459,819 + Shew_0408 0.48 -1.6 460,268 + Shew_0408 0.59 -2.1 460,344 - Shew_0408 0.61 -3.1 460,474 - Shew_0408 0.64 -1.3 460,791 - Shew_0408 0.71 -2.2 460,958 + Shew_0408 0.75 -1.7 460,966 - Shew_0408 0.75 -1.3 461,314 + Shew_0408 0.83 -2.7 461,322 - Shew_0408 0.83 -3.2 461,322 - Shew_0408 0.83 -0.6 461,332 - Shew_0408 0.83 -0.3 461,345 - Shew_0408 0.84 -2.2 461,576 + Shew_0408 0.89 -1.6 461,685 + -0.3 461,769 + +0.5 462,097 - Shew_0409 0.16 -0.0 462,391 - Shew_0409 0.51 +0.4 462,431 + Shew_0409 0.56 +0.1 462,446 - Shew_0409 0.58 -0.1 462,615 + Shew_0409 0.78 +0.2 462,717 + -0.2
Or see this region's nucleotide sequence