Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0370 and Shew_0371 are separated by 124 nucleotides Shew_0371 and Shew_0372 are separated by 94 nucleotides Shew_0372 and Shew_0373 are separated by 21 nucleotides
Shew_0370: Shew_0370 - protoheme IX farnesyltransferase (RefSeq), at 418,183 to 419,109
_0370
Shew_0371: Shew_0371 - AraC family transcriptional regulator (RefSeq), at 419,234 to 420,100
_0371
Shew_0372: Shew_0372 - hypothetical protein (RefSeq), at 420,195 to 420,629
_0372
Shew_0373: Shew_0373 - hypothetical protein (RefSeq), at 420,651 to 421,046
_0373
Position (kb) 419
420
421 Strain fitness (log2 ratio) -1
0
1 at 418.505 kb on + strand, within Shew_0370 at 418.670 kb on - strand, within Shew_0370 at 419.036 kb on - strand at 419.164 kb on + strand at 419.466 kb on + strand, within Shew_0371 at 419.761 kb on + strand, within Shew_0371 at 419.890 kb on + strand, within Shew_0371 at 420.095 kb on + strand at 420.254 kb on + strand, within Shew_0372 at 420.343 kb on + strand, within Shew_0372 at 420.613 kb on + strand at 420.780 kb on + strand, within Shew_0373 at 420.886 kb on + strand, within Shew_0373 at 420.886 kb on + strand, within Shew_0373 at 421.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 418,505 + Shew_0370 0.35 +0.3 418,670 - Shew_0370 0.53 -0.2 419,036 - +0.4 419,164 + +0.1 419,466 + Shew_0371 0.27 +0.0 419,761 + Shew_0371 0.61 -1.1 419,890 + Shew_0371 0.76 +0.1 420,095 + +0.4 420,254 + Shew_0372 0.14 -0.1 420,343 + Shew_0372 0.34 +0.7 420,613 + -0.1 420,780 + Shew_0373 0.33 +0.4 420,886 + Shew_0373 0.59 +0.1 420,886 + Shew_0373 0.59 -0.9 421,058 + +0.7
Or see this region's nucleotide sequence