Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0233 and Shew_0234 are separated by 74 nucleotides Shew_0234 and Shew_0235 are separated by 13 nucleotides Shew_0235 and Shew_0236 are separated by 117 nucleotides
Shew_0233: Shew_0233 - hypothetical protein (RefSeq), at 247,659 to 250,001
_0233
Shew_0234: Shew_0234 - hypothetical protein (RefSeq), at 250,076 to 250,435
_0234
Shew_0235: Shew_0235 - isochorismatase hydrolase (RefSeq), at 250,449 to 251,399
_0235
Shew_0236: Shew_0236 - LysR family transcriptional regulator (RefSeq), at 251,517 to 252,434
_0236
Position (kb) 250
251
252 Strain fitness (log2 ratio) -2
-1
0
1 at 249.635 kb on - strand, within Shew_0233 at 249.635 kb on - strand, within Shew_0233 at 249.818 kb on + strand at 249.982 kb on - strand at 250.144 kb on + strand, within Shew_0234 at 250.151 kb on + strand, within Shew_0234 at 250.179 kb on - strand, within Shew_0234 at 250.224 kb on + strand, within Shew_0234 at 250.481 kb on + strand at 250.659 kb on + strand, within Shew_0235 at 250.659 kb on + strand, within Shew_0235 at 250.662 kb on - strand, within Shew_0235 at 250.667 kb on - strand, within Shew_0235 at 250.667 kb on - strand, within Shew_0235 at 250.936 kb on + strand, within Shew_0235 at 251.742 kb on + strand, within Shew_0236 at 251.760 kb on - strand, within Shew_0236 at 252.100 kb on + strand, within Shew_0236 at 252.182 kb on + strand, within Shew_0236 at 252.243 kb on - strand, within Shew_0236
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 249,635 - Shew_0233 0.84 +0.5 249,635 - Shew_0233 0.84 -0.2 249,818 + +0.8 249,982 - -0.1 250,144 + Shew_0234 0.19 -0.1 250,151 + Shew_0234 0.21 +0.4 250,179 - Shew_0234 0.29 -1.0 250,224 + Shew_0234 0.41 -0.1 250,481 + +0.3 250,659 + Shew_0235 0.22 +0.3 250,659 + Shew_0235 0.22 +0.3 250,662 - Shew_0235 0.22 +0.2 250,667 - Shew_0235 0.23 +0.7 250,667 - Shew_0235 0.23 -0.2 250,936 + Shew_0235 0.51 -0.2 251,742 + Shew_0236 0.25 +0.1 251,760 - Shew_0236 0.26 +0.0 252,100 + Shew_0236 0.64 -2.6 252,182 + Shew_0236 0.72 -0.4 252,243 - Shew_0236 0.79 -0.6
Or see this region's nucleotide sequence