Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0212 and Shew_0213 are separated by 215 nucleotides Shew_0213 and Shew_0214 are separated by 15 nucleotides Shew_0214 and Shew_0215 are separated by 16 nucleotides
Shew_0212: Shew_0212 - type IV pilus secretin PilQ (RefSeq), at 226,517 to 228,568
_0212
Shew_0213: Shew_0213 - shikimate kinase I (RefSeq), at 228,784 to 229,299
_0213
Shew_0214: Shew_0214 - 3-dehydroquinate synthase (RefSeq), at 229,315 to 230,391
_0214
Shew_0215: Shew_0215 - sporulation domain-containing protein (RefSeq), at 230,408 to 231,943
_0215
Position (kb) 229
230
231 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 228.315 kb on - strand, within Shew_0212 at 228.329 kb on - strand, within Shew_0212 at 228.366 kb on + strand at 228.381 kb on - strand at 228.552 kb on + strand at 229.593 kb on - strand, within Shew_0214 at 229.767 kb on + strand, within Shew_0214 at 229.775 kb on - strand, within Shew_0214 at 229.827 kb on - strand, within Shew_0214 at 229.915 kb on - strand, within Shew_0214 at 229.991 kb on - strand, within Shew_0214 at 230.060 kb on - strand, within Shew_0214 at 230.522 kb on + strand at 230.579 kb on - strand, within Shew_0215 at 230.721 kb on + strand, within Shew_0215 at 230.769 kb on - strand, within Shew_0215 at 230.791 kb on + strand, within Shew_0215 at 230.937 kb on - strand, within Shew_0215 at 231.046 kb on - strand, within Shew_0215 at 231.287 kb on - strand, within Shew_0215
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 228,315 - Shew_0212 0.88 -1.6 228,329 - Shew_0212 0.88 +0.1 228,366 + +0.4 228,381 - -1.3 228,552 + +0.0 229,593 - Shew_0214 0.26 -2.8 229,767 + Shew_0214 0.42 -2.8 229,775 - Shew_0214 0.43 -4.1 229,827 - Shew_0214 0.48 -2.8 229,915 - Shew_0214 0.56 -3.0 229,991 - Shew_0214 0.63 -2.8 230,060 - Shew_0214 0.69 +0.4 230,522 + +0.3 230,579 - Shew_0215 0.11 -0.3 230,721 + Shew_0215 0.20 +0.2 230,769 - Shew_0215 0.24 -0.3 230,791 + Shew_0215 0.25 +0.8 230,937 - Shew_0215 0.34 +0.6 231,046 - Shew_0215 0.42 +0.1 231,287 - Shew_0215 0.57 +0.4
Or see this region's nucleotide sequence