Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0135 and Shew_0136 are separated by 162 nucleotides Shew_0136 and Shew_0137 are separated by 139 nucleotides Shew_0137 and Shew_0138 are separated by 80 nucleotides
Shew_0135: Shew_0135 - selenide, water dikinase (RefSeq), at 144,673 to 145,716
_0135
Shew_0136: Shew_0136 - stearoyl-CoA 9-desaturase (RefSeq), at 145,879 to 146,985
_0136
Shew_0137: Shew_0137 - DNA-binding transcriptional repressor FabR (RefSeq), at 147,125 to 147,742
_0137
Shew_0138: Shew_0138 - tRNA (uracil-5-)-methyltransferase (RefSeq), at 147,823 to 148,920
_0138
Position (kb) 145
146
147 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 144.963 kb on + strand, within Shew_0135 at 144.971 kb on - strand, within Shew_0135 at 144.971 kb on - strand, within Shew_0135 at 145.396 kb on + strand, within Shew_0135 at 145.520 kb on + strand, within Shew_0135 at 145.644 kb on - strand at 145.703 kb on - strand at 145.746 kb on + strand at 145.770 kb on - strand at 145.770 kb on - strand at 145.770 kb on - strand at 145.991 kb on + strand, within Shew_0136 at 146.102 kb on + strand, within Shew_0136 at 146.103 kb on + strand, within Shew_0136 at 146.168 kb on + strand, within Shew_0136 at 146.187 kb on + strand, within Shew_0136 at 146.359 kb on - strand, within Shew_0136 at 146.374 kb on - strand, within Shew_0136 at 146.374 kb on - strand, within Shew_0136 at 146.407 kb on - strand, within Shew_0136 at 146.449 kb on + strand, within Shew_0136 at 146.498 kb on - strand, within Shew_0136 at 146.723 kb on + strand, within Shew_0136 at 146.724 kb on - strand, within Shew_0136 at 146.736 kb on + strand, within Shew_0136 at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.052 kb on + strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.060 kb on - strand at 147.255 kb on - strand, within Shew_0137 at 147.334 kb on - strand, within Shew_0137 at 147.427 kb on - strand, within Shew_0137 at 147.474 kb on + strand, within Shew_0137 at 147.515 kb on - strand, within Shew_0137 at 147.548 kb on - strand, within Shew_0137 at 147.721 kb on + strand at 147.807 kb on + strand at 147.921 kb on + strand at 147.921 kb on + strand at 147.928 kb on - strand at 147.928 kb on - strand at 147.947 kb on - strand, within Shew_0138
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 144,963 + Shew_0135 0.28 +1.0 144,971 - Shew_0135 0.29 -0.7 144,971 - Shew_0135 0.29 +0.1 145,396 + Shew_0135 0.69 -0.0 145,520 + Shew_0135 0.81 +0.6 145,644 - +0.6 145,703 - -0.2 145,746 + +0.2 145,770 - -0.8 145,770 - -0.0 145,770 - -0.1 145,991 + Shew_0136 0.10 -0.5 146,102 + Shew_0136 0.20 -1.9 146,103 + Shew_0136 0.20 -0.8 146,168 + Shew_0136 0.26 -3.4 146,187 + Shew_0136 0.28 -1.1 146,359 - Shew_0136 0.43 -1.3 146,374 - Shew_0136 0.45 -2.9 146,374 - Shew_0136 0.45 -1.7 146,407 - Shew_0136 0.48 -1.8 146,449 + Shew_0136 0.51 -1.5 146,498 - Shew_0136 0.56 -1.1 146,723 + Shew_0136 0.76 -1.2 146,724 - Shew_0136 0.76 -1.9 146,736 + Shew_0136 0.77 -1.6 147,052 + +0.7 147,052 + +0.4 147,052 + +0.5 147,052 + +0.9 147,052 + +0.1 147,052 + +0.0 147,052 + -0.2 147,052 + -0.4 147,052 + +0.4 147,052 + +0.3 147,052 + +0.5 147,052 + +0.4 147,060 - +0.4 147,060 - +0.6 147,060 - +0.5 147,060 - -0.9 147,060 - +0.1 147,060 - -0.2 147,060 - +0.6 147,060 - +0.2 147,060 - +0.4 147,060 - +0.1 147,060 - +0.1 147,060 - +0.4 147,255 - Shew_0137 0.21 +1.7 147,334 - Shew_0137 0.34 +1.1 147,427 - Shew_0137 0.49 +1.9 147,474 + Shew_0137 0.56 +1.6 147,515 - Shew_0137 0.63 +1.9 147,548 - Shew_0137 0.68 +1.5 147,721 + -0.2 147,807 + -0.2 147,921 + +0.1 147,921 + +0.1 147,928 - -0.1 147,928 - -0.2 147,947 - Shew_0138 0.11 +0.5
Or see this region's nucleotide sequence