Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0130 and Shew_0131 overlap by 8 nucleotides Shew_0131 and Shew_0132 are separated by 107 nucleotides Shew_0132 and Shew_0133 overlap by 10 nucleotides
Shew_0130: Shew_0130 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 139,630 to 140,442
_0130
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Shew_0132: Shew_0132 - TetR family transcriptional regulator (RefSeq), at 140,998 to 141,654
_0132
Shew_0133: Shew_0133 - phospholipid/glycerol acyltransferase (RefSeq), at 141,645 to 143,507
_0133
Position (kb) 140
141
142 Strain fitness (log2 ratio) -1
0
1 at 140.097 kb on - strand, within Shew_0130 at 140.201 kb on - strand, within Shew_0130 at 140.442 kb on + strand at 140.476 kb on - strand at 140.609 kb on - strand, within Shew_0131 at 140.665 kb on + strand, within Shew_0131 at 140.756 kb on - strand, within Shew_0131 at 140.855 kb on + strand at 141.028 kb on - strand at 141.097 kb on - strand, within Shew_0132 at 141.099 kb on + strand, within Shew_0132 at 141.276 kb on - strand, within Shew_0132 at 141.342 kb on + strand, within Shew_0132 at 141.471 kb on + strand, within Shew_0132 at 141.536 kb on + strand, within Shew_0132 at 141.570 kb on + strand, within Shew_0132 at 141.583 kb on - strand, within Shew_0132 at 141.886 kb on + strand, within Shew_0133 at 141.997 kb on + strand, within Shew_0133 at 142.032 kb on - strand, within Shew_0133 at 142.069 kb on + strand, within Shew_0133 at 142.270 kb on - strand, within Shew_0133 at 142.528 kb on + strand, within Shew_0133 at 142.536 kb on - strand, within Shew_0133 at 142.548 kb on + strand, within Shew_0133
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 140,097 - Shew_0130 0.57 -1.5 140,201 - Shew_0130 0.70 -1.0 140,442 + -0.1 140,476 - +0.4 140,609 - Shew_0131 0.38 -0.0 140,665 + Shew_0131 0.50 -0.3 140,756 - Shew_0131 0.70 +0.8 140,855 + +0.3 141,028 - +0.4 141,097 - Shew_0132 0.15 +0.8 141,099 + Shew_0132 0.15 -0.1 141,276 - Shew_0132 0.42 +0.3 141,342 + Shew_0132 0.52 +0.5 141,471 + Shew_0132 0.72 +0.5 141,536 + Shew_0132 0.82 +0.2 141,570 + Shew_0132 0.87 +0.7 141,583 - Shew_0132 0.89 -0.2 141,886 + Shew_0133 0.13 -0.1 141,997 + Shew_0133 0.19 +0.0 142,032 - Shew_0133 0.21 +0.5 142,069 + Shew_0133 0.23 +1.3 142,270 - Shew_0133 0.34 -0.1 142,528 + Shew_0133 0.47 +0.2 142,536 - Shew_0133 0.48 -0.2 142,548 + Shew_0133 0.48 +0.5
Or see this region's nucleotide sequence