Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0049 and Shew_0050 are separated by 103 nucleotides Shew_0050 and Shew_0051 are separated by 7 nucleotides
Shew_0049: Shew_0049 - choline/carnitine/betaine transporter (RefSeq), at 59,401 to 60,972
_0049
Shew_0050: Shew_0050 - DNA polymerase III subunit epsilon (RefSeq), at 61,076 to 61,795
_0050
Shew_0051: Shew_0051 - cyclic nucleotide-binding protein (RefSeq), at 61,803 to 63,692
_0051
Position (kb) 61
62 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.152 kb on + strand, within Shew_0049 at 60.152 kb on + strand, within Shew_0049 at 60.160 kb on - strand, within Shew_0049 at 60.238 kb on - strand, within Shew_0049 at 60.238 kb on - strand, within Shew_0049 at 60.586 kb on + strand, within Shew_0049 at 60.950 kb on - strand at 60.950 kb on - strand at 61.064 kb on - strand at 61.187 kb on + strand, within Shew_0050 at 61.198 kb on - strand, within Shew_0050 at 61.204 kb on - strand, within Shew_0050 at 61.308 kb on + strand, within Shew_0050 at 61.473 kb on - strand, within Shew_0050 at 61.513 kb on + strand, within Shew_0050 at 61.572 kb on + strand, within Shew_0050 at 61.599 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.736 kb on - strand at 61.812 kb on - strand at 61.921 kb on + strand at 62.300 kb on + strand, within Shew_0051 at 62.300 kb on + strand, within Shew_0051 at 62.574 kb on + strand, within Shew_0051 at 62.695 kb on - strand, within Shew_0051 at 62.769 kb on + strand, within Shew_0051 at 62.778 kb on - strand, within Shew_0051 at 62.778 kb on - strand, within Shew_0051
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 60,152 + Shew_0049 0.48 +0.2 60,152 + Shew_0049 0.48 -0.6 60,160 - Shew_0049 0.48 -0.1 60,238 - Shew_0049 0.53 +0.1 60,238 - Shew_0049 0.53 +1.2 60,586 + Shew_0049 0.75 +0.2 60,950 - -2.2 60,950 - +0.2 61,064 - +0.5 61,187 + Shew_0050 0.15 +1.6 61,198 - Shew_0050 0.17 +0.3 61,204 - Shew_0050 0.18 +0.5 61,308 + Shew_0050 0.32 +0.0 61,473 - Shew_0050 0.55 -0.8 61,513 + Shew_0050 0.61 +2.0 61,572 + Shew_0050 0.69 +0.4 61,599 + Shew_0050 0.73 -0.5 61,616 + Shew_0050 0.75 +1.2 61,616 + Shew_0050 0.75 +0.8 61,736 - -1.2 61,812 - +0.1 61,921 + +0.5 62,300 + Shew_0051 0.26 -2.3 62,300 + Shew_0051 0.26 +0.2 62,574 + Shew_0051 0.41 -0.4 62,695 - Shew_0051 0.47 -0.5 62,769 + Shew_0051 0.51 +0.4 62,778 - Shew_0051 0.52 +1.2 62,778 - Shew_0051 0.52 -0.1
Or see this region's nucleotide sequence