Experiment: Polymyxin B sulfate 20 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0024 and Shew_0025 are separated by 272 nucleotides Shew_0025 and Shew_0026 are separated by 115 nucleotides Shew_0026 and Shew_0027 are separated by 12 nucleotides Shew_0027 and Shew_0028 are separated by 81 nucleotides
Shew_0024: Shew_0024 - ArsR family transcriptional regulator (RefSeq), at 27,605 to 27,910
_0024
Shew_0025: Shew_0025 - protoporphyrinogen oxidase (RefSeq), at 28,183 to 28,716
_0025
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Shew_0027: Shew_0027 - glutathione S-transferase domain-containing protein (RefSeq), at 29,381 to 30,028
_0027
Shew_0028: Shew_0028 - LysR family transcriptional regulator (RefSeq), at 30,110 to 31,009
_0028
Position (kb) 28
29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 27.851 kb on - strand, within Shew_0024 at 28.301 kb on + strand, within Shew_0025 at 28.365 kb on - strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.401 kb on + strand, within Shew_0025 at 28.442 kb on + strand, within Shew_0025 at 28.700 kb on - strand at 28.750 kb on + strand at 29.027 kb on - strand, within Shew_0026 at 29.135 kb on + strand, within Shew_0026 at 29.328 kb on - strand at 29.371 kb on - strand at 29.774 kb on - strand, within Shew_0027 at 29.889 kb on + strand, within Shew_0027 at 30.115 kb on - strand at 30.128 kb on - strand at 30.324 kb on - strand, within Shew_0028
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 20 mg/ml remove 27,851 - Shew_0024 0.80 -2.6 28,301 + Shew_0025 0.22 +0.4 28,365 - Shew_0025 0.34 -0.1 28,401 + Shew_0025 0.41 +0.2 28,401 + Shew_0025 0.41 -1.7 28,401 + Shew_0025 0.41 -0.3 28,442 + Shew_0025 0.49 +0.1 28,700 - -0.2 28,750 + -1.2 29,027 - Shew_0026 0.36 -0.4 29,135 + Shew_0026 0.56 +0.2 29,328 - +0.6 29,371 - +0.5 29,774 - Shew_0027 0.61 +0.0 29,889 + Shew_0027 0.78 +1.2 30,115 - -0.4 30,128 - +1.2 30,324 - Shew_0028 0.24 +0.3
Or see this region's nucleotide sequence