Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_953 and Pf6N2E2_954 are separated by 125 nucleotides Pf6N2E2_954 and Pf6N2E2_955 are separated by 17 nucleotides Pf6N2E2_955 and Pf6N2E2_956 are separated by 7 nucleotides Pf6N2E2_956 and Pf6N2E2_957 are separated by 17 nucleotides Pf6N2E2_957 and Pf6N2E2_958 are separated by 236 nucleotides
Pf6N2E2_953 - FIG00958851: hypothetical protein, at 1,125,205 to 1,125,888
_953
Pf6N2E2_954 - Possible exported protein, at 1,126,014 to 1,126,634
_954
Pf6N2E2_955 - FIG00953508: hypothetical protein, at 1,126,652 to 1,127,065
_955
Pf6N2E2_956 - FIG01210404: hypothetical protein, at 1,127,073 to 1,127,444
_956
Pf6N2E2_957 - hypothetical protein, at 1,127,462 to 1,127,578
_957
Pf6N2E2_958 - hypothetical protein, at 1,127,815 to 1,127,934
_958
Position (kb)
1126
1127
1128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1125.800 kb on + strand, within Pf6N2E2_953 at 1125.801 kb on - strand, within Pf6N2E2_953 at 1125.925 kb on + strand at 1126.003 kb on - strand at 1126.165 kb on + strand, within Pf6N2E2_954 at 1126.166 kb on - strand, within Pf6N2E2_954 at 1126.168 kb on + strand, within Pf6N2E2_954 at 1126.169 kb on - strand, within Pf6N2E2_954 at 1126.169 kb on - strand, within Pf6N2E2_954 at 1126.169 kb on - strand, within Pf6N2E2_954 at 1126.321 kb on + strand, within Pf6N2E2_954 at 1126.375 kb on + strand, within Pf6N2E2_954 at 1126.375 kb on + strand, within Pf6N2E2_954 at 1126.376 kb on - strand, within Pf6N2E2_954 at 1126.376 kb on - strand, within Pf6N2E2_954 at 1126.376 kb on - strand, within Pf6N2E2_954 at 1126.438 kb on + strand, within Pf6N2E2_954 at 1126.438 kb on + strand, within Pf6N2E2_954 at 1126.439 kb on - strand, within Pf6N2E2_954 at 1126.439 kb on - strand, within Pf6N2E2_954 at 1126.439 kb on - strand, within Pf6N2E2_954 at 1126.439 kb on - strand, within Pf6N2E2_954 at 1126.453 kb on + strand, within Pf6N2E2_954 at 1126.454 kb on - strand, within Pf6N2E2_954 at 1126.454 kb on - strand, within Pf6N2E2_954 at 1126.454 kb on - strand, within Pf6N2E2_954 at 1126.454 kb on - strand, within Pf6N2E2_954 at 1126.472 kb on + strand, within Pf6N2E2_954 at 1126.515 kb on + strand, within Pf6N2E2_954 at 1126.515 kb on + strand, within Pf6N2E2_954 at 1126.515 kb on + strand, within Pf6N2E2_954 at 1126.515 kb on + strand, within Pf6N2E2_954 at 1126.576 kb on - strand at 1126.841 kb on + strand, within Pf6N2E2_955 at 1126.841 kb on + strand, within Pf6N2E2_955 at 1126.842 kb on - strand, within Pf6N2E2_955 at 1126.842 kb on - strand, within Pf6N2E2_955 at 1127.168 kb on + strand, within Pf6N2E2_956 at 1127.169 kb on - strand, within Pf6N2E2_956 at 1127.191 kb on + strand, within Pf6N2E2_956 at 1127.191 kb on + strand, within Pf6N2E2_956 at 1127.192 kb on - strand, within Pf6N2E2_956 at 1127.269 kb on + strand, within Pf6N2E2_956 at 1127.275 kb on + strand, within Pf6N2E2_956 at 1127.276 kb on - strand, within Pf6N2E2_956 at 1127.276 kb on - strand, within Pf6N2E2_956 at 1127.603 kb on + strand at 1127.812 kb on - strand at 1127.812 kb on - strand at 1127.877 kb on + strand, within Pf6N2E2_958 at 1127.878 kb on - strand, within Pf6N2E2_958 at 1127.878 kb on - strand, within Pf6N2E2_958 at 1127.878 kb on - strand, within Pf6N2E2_958 at 1127.937 kb on + strand at 1127.938 kb on - strand at 1128.049 kb on - strand at 1128.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 1,125,800 + Pf6N2E2_953 0.87 -0.6 1,125,801 - Pf6N2E2_953 0.87 -2.5 1,125,925 + -1.0 1,126,003 - +1.1 1,126,165 + Pf6N2E2_954 0.24 +0.9 1,126,166 - Pf6N2E2_954 0.24 -0.2 1,126,168 + Pf6N2E2_954 0.25 +0.6 1,126,169 - Pf6N2E2_954 0.25 +0.9 1,126,169 - Pf6N2E2_954 0.25 -0.3 1,126,169 - Pf6N2E2_954 0.25 +0.7 1,126,321 + Pf6N2E2_954 0.49 +0.8 1,126,375 + Pf6N2E2_954 0.58 -0.3 1,126,375 + Pf6N2E2_954 0.58 -0.6 1,126,376 - Pf6N2E2_954 0.58 -0.6 1,126,376 - Pf6N2E2_954 0.58 +0.3 1,126,376 - Pf6N2E2_954 0.58 +0.5 1,126,438 + Pf6N2E2_954 0.68 +0.2 1,126,438 + Pf6N2E2_954 0.68 -0.6 1,126,439 - Pf6N2E2_954 0.68 +0.9 1,126,439 - Pf6N2E2_954 0.68 +1.0 1,126,439 - Pf6N2E2_954 0.68 +0.7 1,126,439 - Pf6N2E2_954 0.68 +0.9 1,126,453 + Pf6N2E2_954 0.71 -0.3 1,126,454 - Pf6N2E2_954 0.71 -0.7 1,126,454 - Pf6N2E2_954 0.71 +0.1 1,126,454 - Pf6N2E2_954 0.71 +1.1 1,126,454 - Pf6N2E2_954 0.71 +0.8 1,126,472 + Pf6N2E2_954 0.74 +0.8 1,126,515 + Pf6N2E2_954 0.81 -0.4 1,126,515 + Pf6N2E2_954 0.81 +1.4 1,126,515 + Pf6N2E2_954 0.81 -0.0 1,126,515 + Pf6N2E2_954 0.81 -0.6 1,126,576 - +0.4 1,126,841 + Pf6N2E2_955 0.46 -0.3 1,126,841 + Pf6N2E2_955 0.46 -0.3 1,126,842 - Pf6N2E2_955 0.46 -0.7 1,126,842 - Pf6N2E2_955 0.46 -0.2 1,127,168 + Pf6N2E2_956 0.26 +0.5 1,127,169 - Pf6N2E2_956 0.26 +1.2 1,127,191 + Pf6N2E2_956 0.32 +1.4 1,127,191 + Pf6N2E2_956 0.32 +1.1 1,127,192 - Pf6N2E2_956 0.32 +1.6 1,127,269 + Pf6N2E2_956 0.53 +0.7 1,127,275 + Pf6N2E2_956 0.54 -0.9 1,127,276 - Pf6N2E2_956 0.55 +0.7 1,127,276 - Pf6N2E2_956 0.55 -0.1 1,127,603 + +0.6 1,127,812 - +2.5 1,127,812 - +0.4 1,127,877 + Pf6N2E2_958 0.52 +1.5 1,127,878 - Pf6N2E2_958 0.53 +0.2 1,127,878 - Pf6N2E2_958 0.53 -1.0 1,127,878 - Pf6N2E2_958 0.53 -0.1 1,127,937 + +1.8 1,127,938 - +0.0 1,128,049 - -0.1 1,128,052 - -0.5
Or see this region's nucleotide sequence