Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_846 and Pf6N2E2_847 are separated by 52 nucleotides Pf6N2E2_847 and Pf6N2E2_848 overlap by 8 nucleotides Pf6N2E2_848 and Pf6N2E2_849 overlap by 19 nucleotides
Pf6N2E2_846 - Universal stress protein family 5, at 991,394 to 992,284
_846
Pf6N2E2_847 - Phosphoribosyl transferase domain protein, at 992,337 to 993,041
_847
Pf6N2E2_848 - hypothetical protein, at 993,034 to 993,729
_848
Pf6N2E2_849 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 993,711 to 995,078
_849
Position (kb) 993
994 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 992.173 kb on + strand, within Pf6N2E2_846 at 992.173 kb on + strand, within Pf6N2E2_846 at 992.173 kb on + strand, within Pf6N2E2_846 at 992.173 kb on + strand, within Pf6N2E2_846 at 992.174 kb on - strand, within Pf6N2E2_846 at 992.174 kb on - strand, within Pf6N2E2_846 at 992.174 kb on - strand, within Pf6N2E2_846 at 992.231 kb on - strand at 992.245 kb on + strand at 992.245 kb on + strand at 992.246 kb on - strand at 992.259 kb on + strand at 992.259 kb on + strand at 992.282 kb on + strand at 992.282 kb on + strand at 992.282 kb on + strand at 992.282 kb on + strand at 992.587 kb on + strand, within Pf6N2E2_847 at 992.588 kb on - strand, within Pf6N2E2_847 at 992.798 kb on - strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.934 kb on + strand, within Pf6N2E2_847 at 992.935 kb on - strand, within Pf6N2E2_847 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.172 kb on + strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.173 kb on - strand, within Pf6N2E2_848 at 993.670 kb on + strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.671 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.705 kb on - strand at 993.727 kb on + strand at 993.728 kb on - strand at 993.874 kb on + strand, within Pf6N2E2_849 at 993.874 kb on + strand, within Pf6N2E2_849 at 994.115 kb on - strand, within Pf6N2E2_849 at 994.318 kb on + strand, within Pf6N2E2_849 at 994.319 kb on - strand, within Pf6N2E2_849 at 994.321 kb on + strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.322 kb on - strand, within Pf6N2E2_849 at 994.483 kb on + strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.484 kb on - strand, within Pf6N2E2_849 at 994.523 kb on - strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.570 kb on + strand, within Pf6N2E2_849 at 994.607 kb on - strand, within Pf6N2E2_849
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 992,173 + Pf6N2E2_846 0.87 -0.6 992,173 + Pf6N2E2_846 0.87 -1.2 992,173 + Pf6N2E2_846 0.87 +0.3 992,173 + Pf6N2E2_846 0.87 +0.9 992,174 - Pf6N2E2_846 0.88 +0.5 992,174 - Pf6N2E2_846 0.88 -1.6 992,174 - Pf6N2E2_846 0.88 -1.0 992,231 - +0.5 992,245 + +0.8 992,245 + -0.6 992,246 - +0.0 992,259 + +0.6 992,259 + -0.8 992,282 + +0.8 992,282 + +0.0 992,282 + -0.9 992,282 + -1.4 992,587 + Pf6N2E2_847 0.35 -0.2 992,588 - Pf6N2E2_847 0.36 -0.4 992,798 - Pf6N2E2_847 0.65 +0.0 992,934 + Pf6N2E2_847 0.85 -0.4 992,934 + Pf6N2E2_847 0.85 +1.0 992,935 - Pf6N2E2_847 0.85 +0.2 993,172 + Pf6N2E2_848 0.20 -0.1 993,172 + Pf6N2E2_848 0.20 -0.1 993,173 - Pf6N2E2_848 0.20 -0.6 993,173 - Pf6N2E2_848 0.20 +0.8 993,670 + -0.1 993,671 - -0.7 993,671 - -0.6 993,671 - -0.2 993,705 - +0.9 993,705 - +0.6 993,705 - -0.0 993,727 + +0.4 993,728 - -0.2 993,874 + Pf6N2E2_849 0.12 +0.0 993,874 + Pf6N2E2_849 0.12 -0.3 994,115 - Pf6N2E2_849 0.30 +2.9 994,318 + Pf6N2E2_849 0.44 -1.5 994,319 - Pf6N2E2_849 0.44 +0.1 994,321 + Pf6N2E2_849 0.45 -2.0 994,322 - Pf6N2E2_849 0.45 -0.4 994,322 - Pf6N2E2_849 0.45 +2.1 994,322 - Pf6N2E2_849 0.45 +0.2 994,322 - Pf6N2E2_849 0.45 +0.4 994,483 + Pf6N2E2_849 0.56 -0.9 994,484 - Pf6N2E2_849 0.57 +0.6 994,484 - Pf6N2E2_849 0.57 +0.4 994,484 - Pf6N2E2_849 0.57 -0.9 994,484 - Pf6N2E2_849 0.57 -0.7 994,523 - Pf6N2E2_849 0.59 +1.2 994,570 + Pf6N2E2_849 0.63 -0.4 994,570 + Pf6N2E2_849 0.63 +1.5 994,607 - Pf6N2E2_849 0.65 -0.3
Or see this region's nucleotide sequence