Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_796 and Pf6N2E2_797 are separated by 15 nucleotides Pf6N2E2_797 and Pf6N2E2_798 are separated by 32 nucleotides Pf6N2E2_798 and Pf6N2E2_799 overlap by 48 nucleotides
Pf6N2E2_796 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 927,383 to 928,276
_796
Pf6N2E2_797 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 928,292 to 930,259
_797
Pf6N2E2_798 - Ku domain protein, at 930,292 to 931,182
_798
Pf6N2E2_799 - hypothetical protein, at 931,135 to 931,344
_799
Position (kb) 928
929
930
931 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 927.293 kb on + strand at 927.294 kb on - strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.408 kb on - strand at 927.408 kb on - strand at 927.408 kb on - strand at 927.425 kb on + strand at 927.426 kb on - strand at 927.455 kb on - strand at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.516 kb on - strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.754 kb on - strand, within Pf6N2E2_796 at 927.754 kb on - strand, within Pf6N2E2_796 at 927.771 kb on + strand, within Pf6N2E2_796 at 927.852 kb on - strand, within Pf6N2E2_796 at 927.908 kb on + strand, within Pf6N2E2_796 at 927.908 kb on + strand, within Pf6N2E2_796 at 927.909 kb on - strand, within Pf6N2E2_796 at 927.932 kb on + strand, within Pf6N2E2_796 at 927.932 kb on + strand, within Pf6N2E2_796 at 927.983 kb on + strand, within Pf6N2E2_796 at 928.006 kb on + strand, within Pf6N2E2_796 at 928.007 kb on - strand, within Pf6N2E2_796 at 928.166 kb on + strand, within Pf6N2E2_796 at 928.174 kb on - strand, within Pf6N2E2_796 at 928.217 kb on + strand at 928.217 kb on + strand at 928.217 kb on + strand at 928.217 kb on + strand at 928.218 kb on - strand at 928.220 kb on + strand at 928.221 kb on - strand at 928.264 kb on + strand at 928.265 kb on - strand at 928.265 kb on - strand at 928.287 kb on + strand at 928.288 kb on - strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.543 kb on - strand, within Pf6N2E2_797 at 928.630 kb on + strand, within Pf6N2E2_797 at 928.630 kb on + strand, within Pf6N2E2_797 at 928.631 kb on - strand, within Pf6N2E2_797 at 929.103 kb on + strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.106 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.456 kb on + strand, within Pf6N2E2_797 at 929.457 kb on - strand, within Pf6N2E2_797 at 929.505 kb on + strand, within Pf6N2E2_797 at 929.505 kb on + strand, within Pf6N2E2_797 at 929.624 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.650 kb on + strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.651 kb on - strand, within Pf6N2E2_797 at 929.652 kb on + strand, within Pf6N2E2_797 at 929.865 kb on + strand, within Pf6N2E2_797 at 929.865 kb on + strand, within Pf6N2E2_797 at 929.866 kb on - strand, within Pf6N2E2_797 at 930.108 kb on + strand at 930.109 kb on - strand at 930.274 kb on + strand at 930.274 kb on + strand at 930.275 kb on - strand at 930.673 kb on + strand, within Pf6N2E2_798 at 930.673 kb on + strand, within Pf6N2E2_798 at 930.673 kb on + strand, within Pf6N2E2_798 at 930.674 kb on - strand, within Pf6N2E2_798 at 930.674 kb on - strand, within Pf6N2E2_798 at 930.815 kb on + strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.968 kb on + strand, within Pf6N2E2_798 at 930.968 kb on + strand, within Pf6N2E2_798
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 927,293 + +1.3 927,294 - +0.4 927,407 + +0.3 927,407 + -0.5 927,407 + -0.6 927,407 + -0.6 927,407 + +1.2 927,408 - -0.4 927,408 - -0.4 927,408 - +0.9 927,425 + +0.1 927,426 - +0.5 927,455 - +0.2 927,515 + Pf6N2E2_796 0.15 +1.7 927,515 + Pf6N2E2_796 0.15 +0.1 927,515 + Pf6N2E2_796 0.15 +1.0 927,515 + Pf6N2E2_796 0.15 -3.4 927,516 - Pf6N2E2_796 0.15 +0.2 927,753 + Pf6N2E2_796 0.41 +0.4 927,753 + Pf6N2E2_796 0.41 -0.8 927,753 + Pf6N2E2_796 0.41 -1.4 927,754 - Pf6N2E2_796 0.41 -0.0 927,754 - Pf6N2E2_796 0.41 +1.2 927,771 + Pf6N2E2_796 0.43 +1.3 927,852 - Pf6N2E2_796 0.52 -0.5 927,908 + Pf6N2E2_796 0.59 -0.1 927,908 + Pf6N2E2_796 0.59 -0.8 927,909 - Pf6N2E2_796 0.59 +0.8 927,932 + Pf6N2E2_796 0.61 +0.1 927,932 + Pf6N2E2_796 0.61 +0.1 927,983 + Pf6N2E2_796 0.67 -0.3 928,006 + Pf6N2E2_796 0.70 +0.1 928,007 - Pf6N2E2_796 0.70 +0.0 928,166 + Pf6N2E2_796 0.88 +0.1 928,174 - Pf6N2E2_796 0.88 -0.5 928,217 + +1.3 928,217 + -0.2 928,217 + +0.5 928,217 + -0.4 928,218 - -0.4 928,220 + +0.4 928,221 - +1.7 928,264 + +0.4 928,265 - +1.1 928,265 - +0.2 928,287 + -0.6 928,288 - -0.0 928,410 + -0.6 928,410 + +0.8 928,410 + +0.4 928,410 + -1.1 928,410 + -1.5 928,410 + +0.6 928,410 + -0.4 928,410 + +1.2 928,410 + -0.8 928,411 - +0.5 928,411 - +1.2 928,411 - +0.6 928,411 - -2.4 928,411 - +0.6 928,411 - +0.5 928,411 - +0.6 928,411 - +1.2 928,543 - Pf6N2E2_797 0.13 -0.4 928,630 + Pf6N2E2_797 0.17 -0.9 928,630 + Pf6N2E2_797 0.17 +0.2 928,631 - Pf6N2E2_797 0.17 -1.1 929,103 + Pf6N2E2_797 0.41 -0.7 929,104 - Pf6N2E2_797 0.41 +0.3 929,104 - Pf6N2E2_797 0.41 -0.7 929,104 - Pf6N2E2_797 0.41 -0.0 929,104 - Pf6N2E2_797 0.41 -0.9 929,104 - Pf6N2E2_797 0.41 +0.8 929,106 + Pf6N2E2_797 0.41 +0.1 929,456 + Pf6N2E2_797 0.59 +0.5 929,456 + Pf6N2E2_797 0.59 +0.1 929,456 + Pf6N2E2_797 0.59 +0.2 929,456 + Pf6N2E2_797 0.59 +0.1 929,456 + Pf6N2E2_797 0.59 +0.5 929,456 + Pf6N2E2_797 0.59 +1.1 929,456 + Pf6N2E2_797 0.59 +0.8 929,456 + Pf6N2E2_797 0.59 +1.4 929,457 - Pf6N2E2_797 0.59 +0.1 929,505 + Pf6N2E2_797 0.62 -0.3 929,505 + Pf6N2E2_797 0.62 +0.6 929,624 + Pf6N2E2_797 0.68 -1.7 929,650 + Pf6N2E2_797 0.69 +0.8 929,650 + Pf6N2E2_797 0.69 +0.4 929,650 + Pf6N2E2_797 0.69 +0.2 929,650 + Pf6N2E2_797 0.69 -0.4 929,650 + Pf6N2E2_797 0.69 +0.2 929,650 + Pf6N2E2_797 0.69 -1.9 929,650 + Pf6N2E2_797 0.69 -1.4 929,650 + Pf6N2E2_797 0.69 +1.0 929,650 + Pf6N2E2_797 0.69 +1.8 929,650 + Pf6N2E2_797 0.69 -0.1 929,650 + Pf6N2E2_797 0.69 +1.1 929,650 + Pf6N2E2_797 0.69 -1.4 929,650 + Pf6N2E2_797 0.69 -0.8 929,650 + Pf6N2E2_797 0.69 +1.1 929,650 + Pf6N2E2_797 0.69 -0.4 929,651 - Pf6N2E2_797 0.69 -0.9 929,651 - Pf6N2E2_797 0.69 -2.8 929,651 - Pf6N2E2_797 0.69 +1.0 929,651 - Pf6N2E2_797 0.69 +0.4 929,651 - Pf6N2E2_797 0.69 -0.8 929,651 - Pf6N2E2_797 0.69 -1.0 929,651 - Pf6N2E2_797 0.69 -0.9 929,651 - Pf6N2E2_797 0.69 +0.6 929,651 - Pf6N2E2_797 0.69 -0.2 929,651 - Pf6N2E2_797 0.69 -0.6 929,651 - Pf6N2E2_797 0.69 -0.7 929,651 - Pf6N2E2_797 0.69 +0.0 929,651 - Pf6N2E2_797 0.69 +0.3 929,651 - Pf6N2E2_797 0.69 -0.2 929,652 + Pf6N2E2_797 0.69 -1.1 929,865 + Pf6N2E2_797 0.80 +0.2 929,865 + Pf6N2E2_797 0.80 -0.9 929,866 - Pf6N2E2_797 0.80 -1.4 930,108 + +0.7 930,109 - +0.2 930,274 + -1.2 930,274 + +0.9 930,275 - +1.8 930,673 + Pf6N2E2_798 0.43 +1.2 930,673 + Pf6N2E2_798 0.43 +0.0 930,673 + Pf6N2E2_798 0.43 -0.9 930,674 - Pf6N2E2_798 0.43 -0.3 930,674 - Pf6N2E2_798 0.43 +1.2 930,815 + Pf6N2E2_798 0.59 -0.6 930,816 - Pf6N2E2_798 0.59 -0.4 930,816 - Pf6N2E2_798 0.59 +1.2 930,816 - Pf6N2E2_798 0.59 +1.2 930,816 - Pf6N2E2_798 0.59 +0.6 930,968 + Pf6N2E2_798 0.76 +0.8 930,968 + Pf6N2E2_798 0.76 -0.2
Or see this region's nucleotide sequence