Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_759

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_758 and Pf6N2E2_759 are separated by 8 nucleotidesPf6N2E2_759 and Pf6N2E2_760 are separated by 163 nucleotides Pf6N2E2_758 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 867,937 to 869,457 _758 Pf6N2E2_759 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 869,466 to 870,275 _759 Pf6N2E2_760 - Na+/H+ antiporter NhaB, at 870,439 to 871,941 _760 Position (kb) 869 870 871Strain fitness (log2 ratio) -2 -1 0 1 2 3at 868.508 kb on - strand, within Pf6N2E2_758at 868.522 kb on + strand, within Pf6N2E2_758at 868.522 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.603 kb on + strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.604 kb on - strand, within Pf6N2E2_758at 868.616 kb on + strand, within Pf6N2E2_758at 868.616 kb on + strand, within Pf6N2E2_758at 868.617 kb on - strand, within Pf6N2E2_758at 868.617 kb on - strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.936 kb on + strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.937 kb on - strand, within Pf6N2E2_758at 868.962 kb on + strand, within Pf6N2E2_758at 869.060 kb on - strand, within Pf6N2E2_758at 869.100 kb on + strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strandat 869.101 kb on - strand, within Pf6N2E2_758at 869.101 kb on - strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.209 kb on + strand, within Pf6N2E2_758at 869.210 kb on - strand, within Pf6N2E2_758at 869.210 kb on - strand, within Pf6N2E2_758at 869.211 kb on + strand, within Pf6N2E2_758at 869.226 kb on - strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.260 kb on + strand, within Pf6N2E2_758at 869.377 kb on + strandat 869.378 kb on - strandat 869.508 kb on - strandat 869.718 kb on + strand, within Pf6N2E2_759at 869.719 kb on - strand, within Pf6N2E2_759at 869.719 kb on - strand, within Pf6N2E2_759at 869.788 kb on + strand, within Pf6N2E2_759at 869.788 kb on + strand, within Pf6N2E2_759at 869.789 kb on - strand, within Pf6N2E2_759at 869.789 kb on - strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.895 kb on + strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 869.896 kb on - strand, within Pf6N2E2_759at 870.018 kb on + strand, within Pf6N2E2_759at 870.258 kb on + strandat 870.258 kb on + strandat 870.335 kb on - strandat 870.335 kb on - strandat 870.335 kb on - strandat 870.434 kb on + strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.435 kb on - strandat 870.440 kb on + strandat 870.440 kb on + strandat 870.440 kb on + strandat 870.441 kb on - strandat 870.449 kb on + strandat 870.450 kb on - strandat 870.450 kb on - strandat 870.450 kb on - strandat 870.462 kb on - strandat 870.492 kb on - strandat 870.522 kb on - strandat 870.693 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.698 kb on + strand, within Pf6N2E2_760at 870.699 kb on - strand, within Pf6N2E2_760at 871.023 kb on + strand, within Pf6N2E2_760at 871.024 kb on - strand, within Pf6N2E2_760at 871.024 kb on - strand, within Pf6N2E2_760at 871.085 kb on + strand, within Pf6N2E2_760at 871.085 kb on + strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760at 871.106 kb on - strand, within Pf6N2E2_760

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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868,508 - Pf6N2E2_758 0.38 +0.1
868,522 + Pf6N2E2_758 0.38 -0.7
868,522 + Pf6N2E2_758 0.38 -0.3
868,603 + Pf6N2E2_758 0.44 -1.6
868,603 + Pf6N2E2_758 0.44 -1.6
868,603 + Pf6N2E2_758 0.44 +0.5
868,604 - Pf6N2E2_758 0.44 +0.8
868,604 - Pf6N2E2_758 0.44 -0.6
868,604 - Pf6N2E2_758 0.44 -0.2
868,604 - Pf6N2E2_758 0.44 -0.9
868,604 - Pf6N2E2_758 0.44 +0.7
868,604 - Pf6N2E2_758 0.44 +0.5
868,604 - Pf6N2E2_758 0.44 +1.0
868,616 + Pf6N2E2_758 0.45 -0.9
868,616 + Pf6N2E2_758 0.45 +1.5
868,617 - Pf6N2E2_758 0.45 +0.7
868,617 - Pf6N2E2_758 0.45 -0.1
868,936 + Pf6N2E2_758 0.66 +1.4
868,936 + Pf6N2E2_758 0.66 +0.6
868,936 + Pf6N2E2_758 0.66 +0.9
868,936 + Pf6N2E2_758 0.66 +0.7
868,936 + Pf6N2E2_758 0.66 +1.2
868,936 + Pf6N2E2_758 0.66 +1.1
868,936 + Pf6N2E2_758 0.66 +1.5
868,936 + Pf6N2E2_758 0.66 -0.2
868,937 - Pf6N2E2_758 0.66 +0.1
868,937 - Pf6N2E2_758 0.66 -1.2
868,937 - Pf6N2E2_758 0.66 +2.2
868,962 + Pf6N2E2_758 0.67 -0.6
869,060 - Pf6N2E2_758 0.74 -0.5
869,100 + Pf6N2E2_758 0.76 -0.3
869,101 - Pf6N2E2_758 0.77 +1.0
869,101 - Pf6N2E2_758 0.77 +1.1
869,101 - Pf6N2E2_758 0.77 -0.1
869,101 - -0.3
869,101 - Pf6N2E2_758 0.77 -0.1
869,101 - Pf6N2E2_758 0.77 +1.2
869,209 + Pf6N2E2_758 0.84 -1.1
869,209 + Pf6N2E2_758 0.84 +0.3
869,209 + Pf6N2E2_758 0.84 +0.6
869,210 - Pf6N2E2_758 0.84 -0.3
869,210 - Pf6N2E2_758 0.84 +0.9
869,211 + Pf6N2E2_758 0.84 +0.5
869,226 - Pf6N2E2_758 0.85 -0.1
869,260 + Pf6N2E2_758 0.87 +1.0
869,260 + Pf6N2E2_758 0.87 -0.6
869,260 + Pf6N2E2_758 0.87 +0.9
869,260 + Pf6N2E2_758 0.87 -0.4
869,377 + -0.1
869,378 - +0.8
869,508 - +0.2
869,718 + Pf6N2E2_759 0.31 -0.3
869,719 - Pf6N2E2_759 0.31 +1.2
869,719 - Pf6N2E2_759 0.31 -0.2
869,788 + Pf6N2E2_759 0.40 -1.8
869,788 + Pf6N2E2_759 0.40 -0.3
869,789 - Pf6N2E2_759 0.40 -0.9
869,789 - Pf6N2E2_759 0.40 -0.0
869,895 + Pf6N2E2_759 0.53 +0.2
869,895 + Pf6N2E2_759 0.53 +0.7
869,895 + Pf6N2E2_759 0.53 +0.8
869,895 + Pf6N2E2_759 0.53 -0.3
869,895 + Pf6N2E2_759 0.53 -0.3
869,896 - Pf6N2E2_759 0.53 +0.7
869,896 - Pf6N2E2_759 0.53 +0.1
869,896 - Pf6N2E2_759 0.53 -1.2
870,018 + Pf6N2E2_759 0.68 -0.0
870,258 + +2.3
870,258 + +0.2
870,335 - -0.7
870,335 - -0.9
870,335 - +0.2
870,434 + +2.2
870,435 - -0.5
870,435 - +1.7
870,435 - -0.2
870,435 - -0.1
870,435 - -0.7
870,440 + -1.2
870,440 + +1.7
870,440 + +0.4
870,441 - +0.3
870,449 + -0.2
870,450 - -0.5
870,450 - +0.6
870,450 - +0.3
870,462 - -0.6
870,492 - +0.7
870,522 - +0.4
870,693 + Pf6N2E2_760 0.17 +0.3
870,698 + Pf6N2E2_760 0.17 +1.4
870,698 + Pf6N2E2_760 0.17 -0.3
870,698 + Pf6N2E2_760 0.17 +0.6
870,698 + Pf6N2E2_760 0.17 +0.5
870,698 + Pf6N2E2_760 0.17 +0.3
870,698 + Pf6N2E2_760 0.17 -0.1
870,698 + Pf6N2E2_760 0.17 +0.2
870,698 + Pf6N2E2_760 0.17 +0.7
870,698 + Pf6N2E2_760 0.17 -0.4
870,699 - Pf6N2E2_760 0.17 +0.1
871,023 + Pf6N2E2_760 0.39 -0.3
871,024 - Pf6N2E2_760 0.39 -0.1
871,024 - Pf6N2E2_760 0.39 +1.1
871,085 + Pf6N2E2_760 0.43 -0.6
871,085 + Pf6N2E2_760 0.43 -0.5
871,106 - Pf6N2E2_760 0.44 +2.8
871,106 - Pf6N2E2_760 0.44 -0.6
871,106 - Pf6N2E2_760 0.44 +0.7
871,106 - Pf6N2E2_760 0.44 -0.8
871,106 - Pf6N2E2_760 0.44 +0.7
871,106 - Pf6N2E2_760 0.44 +0.0
871,106 - Pf6N2E2_760 0.44 -1.2
871,106 - Pf6N2E2_760 0.44 -0.6
871,106 - Pf6N2E2_760 0.44 -0.3

Or see this region's nucleotide sequence