Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_74

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_73 and Pf6N2E2_74 overlap by 17 nucleotidesPf6N2E2_74 and Pf6N2E2_75 are separated by 89 nucleotidesPf6N2E2_75 and Pf6N2E2_76 are separated by 41 nucleotides Pf6N2E2_73 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 115,330 to 116,637 _73 Pf6N2E2_74 - DedD protein, at 116,621 to 117,295 _74 Pf6N2E2_75 - Colicin V production protein, at 117,385 to 117,945 _75 Pf6N2E2_76 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 117,987 to 119,492 _76 Position (kb) 116 117 118Strain fitness (log2 ratio) -1 0 1 2at 116.642 kb on + strandat 117.144 kb on - strand, within Pf6N2E2_74at 117.144 kb on - strand, within Pf6N2E2_74at 117.153 kb on - strand, within Pf6N2E2_74at 117.374 kb on + strandat 117.637 kb on + strand, within Pf6N2E2_75at 117.777 kb on + strand, within Pf6N2E2_75at 117.777 kb on + strand, within Pf6N2E2_75at 117.777 kb on + strand, within Pf6N2E2_75at 117.777 kb on + strand, within Pf6N2E2_75at 117.899 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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116,642 + +1.4
117,144 - Pf6N2E2_74 0.77 +0.1
117,144 - Pf6N2E2_74 0.77 +0.4
117,153 - Pf6N2E2_74 0.79 -1.1
117,374 + -0.2
117,637 + Pf6N2E2_75 0.45 -0.8
117,777 + Pf6N2E2_75 0.70 -0.7
117,777 + Pf6N2E2_75 0.70 +0.3
117,777 + Pf6N2E2_75 0.70 +1.8
117,777 + Pf6N2E2_75 0.70 +1.5
117,899 + -0.2

Or see this region's nucleotide sequence