Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotides Pf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotides Pf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides Pf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotides
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634
_698
Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052
_699
Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557
_700
Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012
_701
Position (kb) 805
806 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.191 kb on - strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.486 kb on - strand, within Pf6N2E2_697 at 804.493 kb on - strand, within Pf6N2E2_697 at 804.681 kb on + strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.777 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.931 kb on - strand, within Pf6N2E2_697 at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.210 kb on + strand, within Pf6N2E2_698 at 805.211 kb on - strand, within Pf6N2E2_698 at 805.623 kb on - strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.769 kb on + strand, within Pf6N2E2_699 at 805.769 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.907 kb on - strand, within Pf6N2E2_699 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.223 kb on + strand, within Pf6N2E2_700 at 806.224 kb on - strand, within Pf6N2E2_700 at 806.257 kb on - strand, within Pf6N2E2_700
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 804,165 + Pf6N2E2_697 0.23 -0.1 804,165 + Pf6N2E2_697 0.23 -0.6 804,165 + Pf6N2E2_697 0.23 -0.8 804,165 + Pf6N2E2_697 0.23 +0.8 804,191 - Pf6N2E2_697 0.25 -0.6 804,456 + Pf6N2E2_697 0.48 +0.1 804,456 + Pf6N2E2_697 0.48 +0.7 804,456 + Pf6N2E2_697 0.48 -0.4 804,457 - Pf6N2E2_697 0.48 -0.3 804,457 - Pf6N2E2_697 0.48 +0.2 804,457 - Pf6N2E2_697 0.48 +1.1 804,457 - Pf6N2E2_697 0.48 -0.1 804,486 - Pf6N2E2_697 0.50 -0.3 804,493 - Pf6N2E2_697 0.51 +1.1 804,681 + Pf6N2E2_697 0.67 -0.3 804,682 - Pf6N2E2_697 0.67 +0.0 804,682 - Pf6N2E2_697 0.67 -0.6 804,777 + Pf6N2E2_697 0.75 +0.2 804,930 + Pf6N2E2_697 0.88 -0.3 804,930 + Pf6N2E2_697 0.88 -0.6 804,930 + Pf6N2E2_697 0.88 -2.9 804,930 + Pf6N2E2_697 0.88 +0.1 804,930 + Pf6N2E2_697 0.88 +0.1 804,930 + Pf6N2E2_697 0.88 -1.5 804,931 - Pf6N2E2_697 0.88 +0.1 805,070 - -1.2 805,070 - -0.2 805,070 - +0.7 805,070 - +0.2 805,210 + Pf6N2E2_698 0.21 -0.8 805,211 - Pf6N2E2_698 0.21 -0.5 805,623 - -0.3 805,637 + -0.7 805,637 + -0.3 805,637 + +0.4 805,637 + +0.6 805,637 + +0.5 805,637 + -0.6 805,637 + +0.4 805,637 + +0.8 805,638 - -1.7 805,638 - -0.8 805,638 - +0.3 805,638 - -0.6 805,638 - -0.9 805,638 - -0.5 805,638 - -0.3 805,638 - +0.4 805,638 - +0.2 805,638 - -0.0 805,769 + Pf6N2E2_699 0.36 +1.4 805,769 + Pf6N2E2_699 0.36 -0.5 805,906 + Pf6N2E2_699 0.67 +0.2 805,906 + Pf6N2E2_699 0.67 -0.9 805,907 - Pf6N2E2_699 0.67 -0.2 806,223 + Pf6N2E2_700 0.35 +0.4 806,223 + Pf6N2E2_700 0.35 +0.2 806,223 + Pf6N2E2_700 0.35 -1.7 806,223 + Pf6N2E2_700 0.35 +0.0 806,223 + Pf6N2E2_700 0.35 -0.7 806,223 + Pf6N2E2_700 0.35 -1.3 806,224 - Pf6N2E2_700 0.35 +0.8 806,257 - Pf6N2E2_700 0.41 +0.8
Or see this region's nucleotide sequence