Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_698

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotidesPf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotidesPf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotidesPf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotides Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069 _697 Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634 _698 Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052 _699 Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557 _700 Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012 _701 Position (kb) 805 806Strain fitness (log2 ratio) -3 -2 -1 0 1at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.165 kb on + strand, within Pf6N2E2_697at 804.191 kb on - strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.456 kb on + strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.457 kb on - strand, within Pf6N2E2_697at 804.486 kb on - strand, within Pf6N2E2_697at 804.493 kb on - strand, within Pf6N2E2_697at 804.681 kb on + strand, within Pf6N2E2_697at 804.682 kb on - strand, within Pf6N2E2_697at 804.682 kb on - strand, within Pf6N2E2_697at 804.777 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.930 kb on + strand, within Pf6N2E2_697at 804.931 kb on - strand, within Pf6N2E2_697at 805.070 kb on - strandat 805.070 kb on - strandat 805.070 kb on - strandat 805.070 kb on - strandat 805.210 kb on + strand, within Pf6N2E2_698at 805.211 kb on - strand, within Pf6N2E2_698at 805.623 kb on - strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.769 kb on + strand, within Pf6N2E2_699at 805.769 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.907 kb on - strand, within Pf6N2E2_699at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.224 kb on - strand, within Pf6N2E2_700at 806.257 kb on - strand, within Pf6N2E2_700

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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804,165 + Pf6N2E2_697 0.23 -0.1
804,165 + Pf6N2E2_697 0.23 -0.6
804,165 + Pf6N2E2_697 0.23 -0.8
804,165 + Pf6N2E2_697 0.23 +0.8
804,191 - Pf6N2E2_697 0.25 -0.6
804,456 + Pf6N2E2_697 0.48 +0.1
804,456 + Pf6N2E2_697 0.48 +0.7
804,456 + Pf6N2E2_697 0.48 -0.4
804,457 - Pf6N2E2_697 0.48 -0.3
804,457 - Pf6N2E2_697 0.48 +0.2
804,457 - Pf6N2E2_697 0.48 +1.1
804,457 - Pf6N2E2_697 0.48 -0.1
804,486 - Pf6N2E2_697 0.50 -0.3
804,493 - Pf6N2E2_697 0.51 +1.1
804,681 + Pf6N2E2_697 0.67 -0.3
804,682 - Pf6N2E2_697 0.67 +0.0
804,682 - Pf6N2E2_697 0.67 -0.6
804,777 + Pf6N2E2_697 0.75 +0.2
804,930 + Pf6N2E2_697 0.88 -0.3
804,930 + Pf6N2E2_697 0.88 -0.6
804,930 + Pf6N2E2_697 0.88 -2.9
804,930 + Pf6N2E2_697 0.88 +0.1
804,930 + Pf6N2E2_697 0.88 +0.1
804,930 + Pf6N2E2_697 0.88 -1.5
804,931 - Pf6N2E2_697 0.88 +0.1
805,070 - -1.2
805,070 - -0.2
805,070 - +0.7
805,070 - +0.2
805,210 + Pf6N2E2_698 0.21 -0.8
805,211 - Pf6N2E2_698 0.21 -0.5
805,623 - -0.3
805,637 + -0.7
805,637 + -0.3
805,637 + +0.4
805,637 + +0.6
805,637 + +0.5
805,637 + -0.6
805,637 + +0.4
805,637 + +0.8
805,638 - -1.7
805,638 - -0.8
805,638 - +0.3
805,638 - -0.6
805,638 - -0.9
805,638 - -0.5
805,638 - -0.3
805,638 - +0.4
805,638 - +0.2
805,638 - -0.0
805,769 + Pf6N2E2_699 0.36 +1.4
805,769 + Pf6N2E2_699 0.36 -0.5
805,906 + Pf6N2E2_699 0.67 +0.2
805,906 + Pf6N2E2_699 0.67 -0.9
805,907 - Pf6N2E2_699 0.67 -0.2
806,223 + Pf6N2E2_700 0.35 +0.4
806,223 + Pf6N2E2_700 0.35 +0.2
806,223 + Pf6N2E2_700 0.35 -1.7
806,223 + Pf6N2E2_700 0.35 +0.0
806,223 + Pf6N2E2_700 0.35 -0.7
806,223 + Pf6N2E2_700 0.35 -1.3
806,224 - Pf6N2E2_700 0.35 +0.8
806,257 - Pf6N2E2_700 0.41 +0.8

Or see this region's nucleotide sequence