Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotides Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides Pf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotides Pf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotides
Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920
_638
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822
_641
Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801
_642
Position (kb) 731
732
733 Strain fitness (log2 ratio) -2
-1
0
1
2 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 731.029 kb on - strand at 731.103 kb on - strand at 731.169 kb on + strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.976 kb on - strand, within Pf6N2E2_640 at 732.228 kb on + strand at 732.229 kb on - strand at 732.253 kb on - strand at 732.325 kb on + strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.455 kb on - strand, within Pf6N2E2_641 at 732.477 kb on + strand, within Pf6N2E2_641 at 732.622 kb on + strand, within Pf6N2E2_641 at 732.623 kb on - strand, within Pf6N2E2_641 at 732.709 kb on - strand, within Pf6N2E2_641
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 730,801 + Pf6N2E2_638 0.73 -0.2 730,801 + Pf6N2E2_638 0.73 +0.1 730,802 - Pf6N2E2_638 0.73 -0.5 730,802 - Pf6N2E2_638 0.73 +0.0 731,029 - +0.9 731,103 - +1.3 731,169 + Pf6N2E2_639 0.20 +0.0 731,170 - Pf6N2E2_639 0.20 +0.6 731,170 - Pf6N2E2_639 0.20 +0.4 731,170 - Pf6N2E2_639 0.20 +0.3 731,170 - Pf6N2E2_639 0.20 +0.0 731,495 + -1.8 731,528 + -0.5 731,528 + +0.0 731,529 - -0.3 731,540 - -2.0 731,540 - +0.7 731,572 + +1.0 731,573 - +0.4 731,573 - +0.3 731,803 + Pf6N2E2_640 0.24 -0.7 731,803 + Pf6N2E2_640 0.24 -0.9 731,804 - Pf6N2E2_640 0.25 -0.9 731,882 + Pf6N2E2_640 0.37 -0.3 731,975 + Pf6N2E2_640 0.52 +0.4 731,975 + Pf6N2E2_640 0.52 -2.0 731,976 - Pf6N2E2_640 0.52 -0.5 732,228 + +2.3 732,229 - -0.2 732,253 - +0.2 732,325 + Pf6N2E2_641 0.14 +0.5 732,326 - Pf6N2E2_641 0.14 +0.5 732,326 - Pf6N2E2_641 0.14 +0.9 732,401 - Pf6N2E2_641 0.27 +0.2 732,401 - Pf6N2E2_641 0.27 -1.3 732,454 + Pf6N2E2_641 0.36 +0.0 732,454 + Pf6N2E2_641 0.36 -0.3 732,454 + Pf6N2E2_641 0.36 +0.9 732,454 + Pf6N2E2_641 0.36 +0.0 732,454 + Pf6N2E2_641 0.36 +0.2 732,455 - Pf6N2E2_641 0.36 +1.4 732,477 + Pf6N2E2_641 0.40 +0.1 732,622 + Pf6N2E2_641 0.65 +0.2 732,623 - Pf6N2E2_641 0.65 +0.8 732,709 - Pf6N2E2_641 0.80 +0.7
Or see this region's nucleotide sequence