Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_622

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_621 and Pf6N2E2_622 overlap by 4 nucleotidesPf6N2E2_622 and Pf6N2E2_623 overlap by 8 nucleotidesPf6N2E2_623 and Pf6N2E2_624 overlap by 4 nucleotides Pf6N2E2_621 - TPR domain protein in aerotolerance operon, at 707,263 to 708,999 _621 Pf6N2E2_622 - BatA (Bacteroides aerotolerance operon), at 708,996 to 710,075 _622 Pf6N2E2_623 - FIG00954674: hypothetical protein, at 710,068 to 710,562 _623 Pf6N2E2_624 - hypothetical protein PA3071, at 710,559 to 711,503 _624 Position (kb) 708 709 710 711Strain fitness (log2 ratio) -2 -1 0 1at 707.996 kb on - strand, within Pf6N2E2_621at 708.275 kb on + strand, within Pf6N2E2_621at 708.567 kb on + strand, within Pf6N2E2_621at 708.634 kb on + strand, within Pf6N2E2_621at 708.634 kb on + strand, within Pf6N2E2_621at 708.635 kb on - strand, within Pf6N2E2_621at 708.861 kb on + strandat 708.862 kb on - strandat 708.862 kb on - strandat 708.950 kb on - strandat 708.950 kb on - strandat 708.958 kb on + strandat 708.959 kb on - strandat 709.165 kb on + strand, within Pf6N2E2_622at 709.166 kb on - strand, within Pf6N2E2_622at 709.245 kb on + strand, within Pf6N2E2_622at 709.639 kb on + strand, within Pf6N2E2_622at 709.639 kb on + strand, within Pf6N2E2_622at 709.640 kb on - strand, within Pf6N2E2_622at 710.168 kb on - strand, within Pf6N2E2_623at 710.628 kb on - strandat 711.067 kb on + strand, within Pf6N2E2_624

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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707,996 - Pf6N2E2_621 0.42 -0.9
708,275 + Pf6N2E2_621 0.58 -0.5
708,567 + Pf6N2E2_621 0.75 +0.0
708,634 + Pf6N2E2_621 0.79 -1.1
708,634 + Pf6N2E2_621 0.79 -0.8
708,635 - Pf6N2E2_621 0.79 +0.0
708,861 + -2.6
708,862 - -0.5
708,862 - -1.9
708,950 - -1.1
708,950 - -1.3
708,958 + -1.7
708,959 - -1.1
709,165 + Pf6N2E2_622 0.16 -2.2
709,166 - Pf6N2E2_622 0.16 -1.2
709,245 + Pf6N2E2_622 0.23 -1.9
709,639 + Pf6N2E2_622 0.60 -0.5
709,639 + Pf6N2E2_622 0.60 +0.5
709,640 - Pf6N2E2_622 0.60 -0.3
710,168 - Pf6N2E2_623 0.20 +0.2
710,628 - -0.8
711,067 + Pf6N2E2_624 0.54 -1.5

Or see this region's nucleotide sequence