Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_620

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_619 and Pf6N2E2_620 are separated by 337 nucleotidesPf6N2E2_620 and Pf6N2E2_621 overlap by 4 nucleotides Pf6N2E2_619 - Exonuclease SbcD, at 704,047 to 705,291 _619 Pf6N2E2_620 - BatD, at 705,629 to 707,266 _620 Pf6N2E2_621 - TPR domain protein in aerotolerance operon, at 707,263 to 708,999 _621 Position (kb) 705 706 707 708Strain fitness (log2 ratio) -3 -2 -1 0 1at 704.770 kb on - strand, within Pf6N2E2_619at 705.102 kb on - strand, within Pf6N2E2_619at 705.147 kb on + strand, within Pf6N2E2_619at 705.296 kb on + strandat 705.296 kb on + strandat 705.579 kb on + strandat 705.630 kb on + strandat 705.631 kb on - strandat 705.754 kb on - strandat 705.892 kb on + strand, within Pf6N2E2_620at 705.893 kb on - strand, within Pf6N2E2_620at 705.893 kb on - strand, within Pf6N2E2_620at 706.036 kb on - strand, within Pf6N2E2_620at 706.488 kb on + strand, within Pf6N2E2_620at 706.653 kb on + strand, within Pf6N2E2_620at 706.653 kb on + strand, within Pf6N2E2_620at 706.654 kb on - strand, within Pf6N2E2_620at 706.654 kb on - strand, within Pf6N2E2_620at 706.710 kb on + strand, within Pf6N2E2_620at 706.782 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.836 kb on + strand, within Pf6N2E2_620at 706.837 kb on - strand, within Pf6N2E2_620at 706.837 kb on - strand, within Pf6N2E2_620at 706.837 kb on - strand, within Pf6N2E2_620at 706.837 kb on - strand, within Pf6N2E2_620at 707.247 kb on + strandat 707.247 kb on + strandat 707.248 kb on - strandat 707.248 kb on - strandat 707.313 kb on + strandat 707.458 kb on + strand, within Pf6N2E2_621at 707.458 kb on + strand, within Pf6N2E2_621at 707.458 kb on + strand, within Pf6N2E2_621at 707.459 kb on - strand, within Pf6N2E2_621at 707.723 kb on + strand, within Pf6N2E2_621at 707.723 kb on + strand, within Pf6N2E2_621at 707.723 kb on + strand, within Pf6N2E2_621at 707.723 kb on + strand, within Pf6N2E2_621at 707.780 kb on + strand, within Pf6N2E2_621at 707.780 kb on + strand, within Pf6N2E2_621at 707.781 kb on - strand, within Pf6N2E2_621at 707.995 kb on + strand, within Pf6N2E2_621at 707.995 kb on + strand, within Pf6N2E2_621at 707.996 kb on - strand, within Pf6N2E2_621

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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704,770 - Pf6N2E2_619 0.58 +0.3
705,102 - Pf6N2E2_619 0.85 +0.1
705,147 + Pf6N2E2_619 0.88 -0.4
705,296 + +1.2
705,296 + -0.6
705,579 + -1.3
705,630 + +1.0
705,631 - +0.7
705,754 - -0.6
705,892 + Pf6N2E2_620 0.16 +0.0
705,893 - Pf6N2E2_620 0.16 -1.1
705,893 - Pf6N2E2_620 0.16 -1.9
706,036 - Pf6N2E2_620 0.25 -1.2
706,488 + Pf6N2E2_620 0.52 -1.9
706,653 + Pf6N2E2_620 0.63 -2.0
706,653 + Pf6N2E2_620 0.63 -0.1
706,654 - Pf6N2E2_620 0.63 -1.3
706,654 - Pf6N2E2_620 0.63 -0.3
706,710 + Pf6N2E2_620 0.66 -1.1
706,782 + Pf6N2E2_620 0.70 -0.3
706,836 + Pf6N2E2_620 0.74 -0.3
706,836 + Pf6N2E2_620 0.74 -2.6
706,836 + Pf6N2E2_620 0.74 -1.3
706,836 + Pf6N2E2_620 0.74 +0.3
706,836 + Pf6N2E2_620 0.74 -1.5
706,836 + Pf6N2E2_620 0.74 -0.5
706,836 + Pf6N2E2_620 0.74 -0.8
706,836 + Pf6N2E2_620 0.74 -0.2
706,836 + Pf6N2E2_620 0.74 -1.6
706,836 + Pf6N2E2_620 0.74 -0.8
706,837 - Pf6N2E2_620 0.74 -0.1
706,837 - Pf6N2E2_620 0.74 -0.7
706,837 - Pf6N2E2_620 0.74 -0.7
706,837 - Pf6N2E2_620 0.74 -0.3
707,247 + -0.6
707,247 + +0.2
707,248 - -0.3
707,248 - -0.3
707,313 + -0.6
707,458 + Pf6N2E2_621 0.11 -0.1
707,458 + Pf6N2E2_621 0.11 -1.9
707,458 + Pf6N2E2_621 0.11 +0.0
707,459 - Pf6N2E2_621 0.11 -1.3
707,723 + Pf6N2E2_621 0.26 -0.3
707,723 + Pf6N2E2_621 0.26 -0.5
707,723 + Pf6N2E2_621 0.26 -0.1
707,723 + Pf6N2E2_621 0.26 -3.3
707,780 + Pf6N2E2_621 0.30 -0.8
707,780 + Pf6N2E2_621 0.30 -1.3
707,781 - Pf6N2E2_621 0.30 +0.2
707,995 + Pf6N2E2_621 0.42 +0.7
707,995 + Pf6N2E2_621 0.42 -0.7
707,996 - Pf6N2E2_621 0.42 -0.9

Or see this region's nucleotide sequence