Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotides Pf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides Pf6N2E2_614 and Pf6N2E2_615 are separated by 156 nucleotides Pf6N2E2_615 and Pf6N2E2_616 are separated by 173 nucleotides Pf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotides
Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) <small>(from data)</small>, at 695,832 to 697,412
_612
Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869
_613
Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031
_614
Pf6N2E2_615 - FIG00957600: hypothetical protein, at 699,188 to 699,367
_615
Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666
_616
Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412
_617
Position (kb) 697
698
699
700 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 696.858 kb on + strand, within Pf6N2E2_612 at 697.705 kb on - strand, within Pf6N2E2_613 at 697.985 kb on + strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614 at 699.067 kb on + strand at 699.068 kb on - strand at 699.068 kb on - strand at 699.098 kb on - strand at 699.299 kb on + strand, within Pf6N2E2_615 at 699.299 kb on + strand, within Pf6N2E2_615 at 699.300 kb on - strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.312 kb on - strand, within Pf6N2E2_615 at 699.335 kb on + strand, within Pf6N2E2_615 at 699.527 kb on + strand at 699.527 kb on + strand at 699.528 kb on - strand at 699.578 kb on - strand, within Pf6N2E2_616 at 699.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 696,858 + Pf6N2E2_612 0.65 -0.0 697,705 - Pf6N2E2_613 0.51 +0.2 697,985 + Pf6N2E2_614 0.11 -0.3 697,986 - Pf6N2E2_614 0.11 +0.3 697,986 - Pf6N2E2_614 0.11 -1.5 697,986 - Pf6N2E2_614 0.11 -1.8 697,986 - Pf6N2E2_614 0.11 +0.4 698,153 + Pf6N2E2_614 0.25 -2.0 698,153 + Pf6N2E2_614 0.25 -1.5 698,266 + Pf6N2E2_614 0.35 -1.4 698,266 + Pf6N2E2_614 0.35 -1.1 698,270 + Pf6N2E2_614 0.35 -0.8 698,270 + Pf6N2E2_614 0.35 +0.3 698,270 + Pf6N2E2_614 0.35 -1.3 698,271 - Pf6N2E2_614 0.35 -0.0 698,271 - Pf6N2E2_614 0.35 -0.5 698,438 + Pf6N2E2_614 0.49 -3.3 698,438 + Pf6N2E2_614 0.49 -2.5 698,438 + Pf6N2E2_614 0.49 +0.6 698,438 + Pf6N2E2_614 0.49 +0.3 698,439 - Pf6N2E2_614 0.50 -1.2 698,439 - Pf6N2E2_614 0.50 -0.6 698,439 - Pf6N2E2_614 0.50 +0.1 698,439 - Pf6N2E2_614 0.50 -0.5 698,439 - Pf6N2E2_614 0.50 +0.1 698,439 - Pf6N2E2_614 0.50 +0.4 698,472 + Pf6N2E2_614 0.52 -1.3 698,472 + Pf6N2E2_614 0.52 -0.8 698,472 + Pf6N2E2_614 0.52 -1.9 698,472 + Pf6N2E2_614 0.52 -0.3 698,472 + Pf6N2E2_614 0.52 -0.8 698,473 - Pf6N2E2_614 0.52 -0.6 698,473 - Pf6N2E2_614 0.52 -0.3 698,756 + Pf6N2E2_614 0.77 -1.0 698,756 + Pf6N2E2_614 0.77 +0.1 699,067 + -0.2 699,068 - -0.3 699,068 - +0.8 699,098 - +0.4 699,299 + Pf6N2E2_615 0.62 -0.2 699,299 + Pf6N2E2_615 0.62 +0.5 699,300 - Pf6N2E2_615 0.62 -0.5 699,311 + Pf6N2E2_615 0.68 +0.6 699,311 + Pf6N2E2_615 0.68 +0.5 699,312 - Pf6N2E2_615 0.69 +1.7 699,335 + Pf6N2E2_615 0.82 +0.4 699,527 + +0.4 699,527 + +0.8 699,528 - -1.1 699,578 - Pf6N2E2_616 0.29 +1.4 699,812 - +0.1
Or see this region's nucleotide sequence