Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6174 and Pf6N2E2_6175 are separated by 166 nucleotides Pf6N2E2_6175 and Pf6N2E2_6084 are separated by 115 nucleotides Pf6N2E2_6084 and Pf6N2E2_6085 are separated by 142 nucleotides
Pf6N2E2_6174 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 6,905,531 to 6,908,422
_6174
Pf6N2E2_6175 - 5S RNA, at 6,908,589 to 6,908,708
_6175
Pf6N2E2_6084 - FIG00638667: hypothetical protein, at 6,908,824 to 6,909,225
_6084
Pf6N2E2_6085 - Aha1 domain superfamily, at 6,909,368 to 6,909,826
_6085
Position (kb)
6909
6910 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6908.796 kb on - strand at 6908.874 kb on - strand, within Pf6N2E2_6084 at 6908.874 kb on - strand, within Pf6N2E2_6084 at 6908.901 kb on + strand, within Pf6N2E2_6084 at 6908.901 kb on + strand, within Pf6N2E2_6084 at 6908.927 kb on + strand, within Pf6N2E2_6084 at 6908.927 kb on + strand, within Pf6N2E2_6084 at 6909.074 kb on + strand, within Pf6N2E2_6084 at 6909.074 kb on + strand, within Pf6N2E2_6084 at 6909.074 kb on + strand, within Pf6N2E2_6084 at 6909.074 kb on + strand, within Pf6N2E2_6084 at 6909.180 kb on + strand, within Pf6N2E2_6084 at 6909.268 kb on + strand at 6909.269 kb on - strand at 6909.269 kb on - strand at 6909.306 kb on + strand at 6909.307 kb on - strand at 6909.307 kb on - strand at 6909.334 kb on + strand at 6909.334 kb on + strand at 6909.334 kb on + strand at 6909.334 kb on + strand at 6909.334 kb on + strand at 6909.334 kb on + strand at 6909.335 kb on - strand at 6909.343 kb on - strand at 6909.343 kb on - strand at 6909.531 kb on - strand, within Pf6N2E2_6085 at 6909.531 kb on - strand, within Pf6N2E2_6085 at 6909.615 kb on - strand, within Pf6N2E2_6085 at 6909.827 kb on + strand at 6909.828 kb on - strand at 6909.872 kb on + strand at 6909.884 kb on + strand at 6909.896 kb on + strand at 6909.896 kb on + strand at 6909.896 kb on + strand at 6909.896 kb on + strand at 6909.897 kb on - strand at 6909.897 kb on - strand at 6909.897 kb on - strand at 6909.897 kb on - strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.900 kb on + strand at 6909.901 kb on - strand at 6909.901 kb on - strand at 6910.018 kb on + strand at 6910.082 kb on + strand at 6910.082 kb on + strand at 6910.082 kb on + strand at 6910.083 kb on - strand at 6910.096 kb on + strand at 6910.096 kb on + strand at 6910.096 kb on + strand at 6910.097 kb on - strand at 6910.144 kb on + strand at 6910.144 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.149 kb on + strand at 6910.150 kb on - strand at 6910.150 kb on - strand at 6910.150 kb on - strand at 6910.150 kb on - strand at 6910.166 kb on + strand at 6910.167 kb on - strand at 6910.684 kb on - strand at 6910.795 kb on + strand at 6910.810 kb on + strand at 6910.810 kb on + strand at 6910.810 kb on + strand at 6910.810 kb on + strand at 6910.811 kb on - strand at 6910.811 kb on - strand at 6910.811 kb on - strand at 6910.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,908,796 - +0.4 6,908,874 - Pf6N2E2_6084 0.12 -1.1 6,908,874 - Pf6N2E2_6084 0.12 +0.7 6,908,901 + Pf6N2E2_6084 0.19 -0.4 6,908,901 + Pf6N2E2_6084 0.19 -0.0 6,908,927 + Pf6N2E2_6084 0.26 +2.5 6,908,927 + Pf6N2E2_6084 0.26 +0.8 6,909,074 + Pf6N2E2_6084 0.62 +1.2 6,909,074 + Pf6N2E2_6084 0.62 -0.1 6,909,074 + Pf6N2E2_6084 0.62 +1.1 6,909,074 + Pf6N2E2_6084 0.62 +0.1 6,909,180 + Pf6N2E2_6084 0.89 +0.3 6,909,268 + +2.2 6,909,269 - -2.3 6,909,269 - -0.3 6,909,306 + +1.2 6,909,307 - -0.3 6,909,307 - -1.7 6,909,334 + -0.4 6,909,334 + -1.1 6,909,334 + +0.5 6,909,334 + -0.5 6,909,334 + -0.9 6,909,334 + -0.3 6,909,335 - -0.1 6,909,343 - -0.3 6,909,343 - +0.0 6,909,531 - Pf6N2E2_6085 0.36 -1.1 6,909,531 - Pf6N2E2_6085 0.36 +0.1 6,909,615 - Pf6N2E2_6085 0.54 -0.6 6,909,827 + +1.1 6,909,828 - +0.2 6,909,872 + +0.3 6,909,884 + +0.0 6,909,896 + -0.2 6,909,896 + +0.7 6,909,896 + +1.2 6,909,896 + -0.9 6,909,897 - +0.6 6,909,897 - +1.0 6,909,897 - +0.5 6,909,897 - -0.8 6,909,900 + +0.9 6,909,900 + +0.2 6,909,900 + +1.1 6,909,900 + +0.9 6,909,900 + -0.1 6,909,900 + +0.3 6,909,900 + +1.1 6,909,900 + +0.8 6,909,900 + -0.2 6,909,900 + +0.2 6,909,900 + +0.3 6,909,901 - -1.1 6,909,901 - +0.9 6,910,018 + +0.9 6,910,082 + +0.8 6,910,082 + -0.4 6,910,082 + +0.3 6,910,083 - +1.3 6,910,096 + +1.1 6,910,096 + -0.9 6,910,096 + +0.9 6,910,097 - +0.5 6,910,144 + +0.8 6,910,144 + +0.5 6,910,149 + -0.4 6,910,149 + +0.2 6,910,149 + -0.3 6,910,149 + -0.2 6,910,149 + +0.1 6,910,149 + -3.1 6,910,149 + +0.8 6,910,150 - +0.6 6,910,150 - -0.5 6,910,150 - +0.3 6,910,150 - -0.9 6,910,166 + +3.5 6,910,167 - +2.2 6,910,684 - +0.1 6,910,795 + -0.3 6,910,810 + -0.5 6,910,810 + -0.4 6,910,810 + +0.2 6,910,810 + +0.2 6,910,811 - +1.0 6,910,811 - -0.9 6,910,811 - -0.0 6,910,811 - +1.0
Or see this region's nucleotide sequence