Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6057 and Pf6N2E2_6058 overlap by 1 nucleotides Pf6N2E2_6058 and Pf6N2E2_6059 are separated by 32 nucleotides Pf6N2E2_6059 and Pf6N2E2_6060 are separated by 280 nucleotides
Pf6N2E2_6057 - FIG002781: Alpha-L-glutamate ligase family protein, at 6,876,813 to 6,877,799
_6057
Pf6N2E2_6058 - FIG139976: hypothetical protein, at 6,877,799 to 6,879,304
_6058
Pf6N2E2_6059 - FIG008443: hypothetical protein, at 6,879,337 to 6,879,873
_6059
Pf6N2E2_6060 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 6,880,154 to 6,880,864
_6060
Position (kb)
6877
6878
6879
6880 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6876.834 kb on - strand at 6877.046 kb on - strand, within Pf6N2E2_6057 at 6877.066 kb on + strand at 6877.066 kb on + strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.067 kb on - strand, within Pf6N2E2_6057 at 6877.711 kb on + strand at 6877.711 kb on + strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.712 kb on - strand at 6877.723 kb on + strand at 6877.723 kb on + strand at 6877.733 kb on + strand at 6877.926 kb on + strand at 6877.926 kb on + strand at 6877.927 kb on - strand at 6878.622 kb on + strand, within Pf6N2E2_6058 at 6878.623 kb on - strand, within Pf6N2E2_6058 at 6878.973 kb on + strand, within Pf6N2E2_6058 at 6878.998 kb on - strand, within Pf6N2E2_6058 at 6879.000 kb on + strand, within Pf6N2E2_6058 at 6879.001 kb on - strand, within Pf6N2E2_6058 at 6879.001 kb on - strand, within Pf6N2E2_6058 at 6879.001 kb on - strand, within Pf6N2E2_6058 at 6879.001 kb on - strand, within Pf6N2E2_6058 at 6879.117 kb on + strand, within Pf6N2E2_6058 at 6879.117 kb on + strand, within Pf6N2E2_6058 at 6879.118 kb on - strand, within Pf6N2E2_6058 at 6879.118 kb on - strand, within Pf6N2E2_6058 at 6879.118 kb on - strand, within Pf6N2E2_6058 at 6879.338 kb on + strand at 6879.338 kb on + strand at 6879.339 kb on - strand at 6879.386 kb on + strand at 6879.386 kb on + strand at 6879.390 kb on + strand at 6879.390 kb on + strand at 6879.390 kb on + strand at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.391 kb on - strand, within Pf6N2E2_6059 at 6879.654 kb on - strand, within Pf6N2E2_6059 at 6879.779 kb on + strand, within Pf6N2E2_6059 at 6879.794 kb on + strand, within Pf6N2E2_6059 at 6879.795 kb on - strand, within Pf6N2E2_6059 at 6879.842 kb on + strand at 6879.842 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.873 kb on + strand at 6879.874 kb on - strand at 6879.920 kb on + strand at 6879.920 kb on + strand at 6879.920 kb on + strand at 6879.950 kb on + strand at 6879.951 kb on - strand at 6879.952 kb on + strand at 6879.983 kb on + strand at 6879.984 kb on - strand at 6880.060 kb on + strand at 6880.229 kb on - strand, within Pf6N2E2_6060 at 6880.301 kb on + strand, within Pf6N2E2_6060
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,876,834 - +0.1 6,877,046 - Pf6N2E2_6057 0.24 -0.1 6,877,066 + -1.1 6,877,066 + Pf6N2E2_6057 0.26 -0.1 6,877,067 - Pf6N2E2_6057 0.26 -0.3 6,877,067 - Pf6N2E2_6057 0.26 -0.8 6,877,067 - Pf6N2E2_6057 0.26 -1.1 6,877,711 + -1.1 6,877,711 + -1.8 6,877,712 - +1.7 6,877,712 - -1.3 6,877,712 - +0.6 6,877,712 - +0.2 6,877,723 + -1.4 6,877,723 + +0.5 6,877,733 + -0.1 6,877,926 + -1.2 6,877,926 + -0.3 6,877,927 - -1.7 6,878,622 + Pf6N2E2_6058 0.55 -0.7 6,878,623 - Pf6N2E2_6058 0.55 -1.1 6,878,973 + Pf6N2E2_6058 0.78 -0.7 6,878,998 - Pf6N2E2_6058 0.80 -0.5 6,879,000 + Pf6N2E2_6058 0.80 -0.6 6,879,001 - Pf6N2E2_6058 0.80 -1.3 6,879,001 - Pf6N2E2_6058 0.80 -1.0 6,879,001 - Pf6N2E2_6058 0.80 -0.1 6,879,001 - Pf6N2E2_6058 0.80 -0.1 6,879,117 + Pf6N2E2_6058 0.88 +0.6 6,879,117 + Pf6N2E2_6058 0.88 -1.7 6,879,118 - Pf6N2E2_6058 0.88 -0.1 6,879,118 - Pf6N2E2_6058 0.88 -1.7 6,879,118 - Pf6N2E2_6058 0.88 -0.1 6,879,338 + -0.5 6,879,338 + -1.7 6,879,339 - -2.1 6,879,386 + +0.2 6,879,386 + +0.1 6,879,390 + -0.8 6,879,390 + +1.6 6,879,390 + -0.3 6,879,391 - Pf6N2E2_6059 0.10 +0.6 6,879,391 - Pf6N2E2_6059 0.10 -0.8 6,879,391 - Pf6N2E2_6059 0.10 +0.8 6,879,391 - Pf6N2E2_6059 0.10 +0.5 6,879,654 - Pf6N2E2_6059 0.59 +1.9 6,879,779 + Pf6N2E2_6059 0.82 -0.1 6,879,794 + Pf6N2E2_6059 0.85 +0.8 6,879,795 - Pf6N2E2_6059 0.85 +0.8 6,879,842 + +0.3 6,879,842 + -0.1 6,879,873 + -0.7 6,879,873 + -0.4 6,879,873 + +0.9 6,879,873 + -0.7 6,879,873 + -0.2 6,879,874 - +0.8 6,879,920 + +0.5 6,879,920 + -0.7 6,879,920 + -1.7 6,879,950 + -1.7 6,879,951 - +0.5 6,879,952 + -0.3 6,879,983 + -0.8 6,879,984 - -1.1 6,880,060 + +0.2 6,880,229 - Pf6N2E2_6060 0.11 +1.4 6,880,301 + Pf6N2E2_6060 0.21 +0.6
Or see this region's nucleotide sequence