Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5990 and Pf6N2E2_5991 are separated by 148 nucleotides Pf6N2E2_5991 and Pf6N2E2_5992 are separated by 54 nucleotides Pf6N2E2_5992 and Pf6N2E2_5993 are separated by 141 nucleotides Pf6N2E2_5993 and Pf6N2E2_5994 are separated by 201 nucleotides
Pf6N2E2_5990 - Translation initiation factor 2 (IF-2; GTPase), at 6,807,972 to 6,808,448
_5990
Pf6N2E2_5991 - proteinase inhibitor, at 6,808,597 to 6,808,851
_5991
Pf6N2E2_5992 - Collagenase and related proteases, at 6,808,906 to 6,809,514
_5992
Pf6N2E2_5993 - FIG00957412: hypothetical protein, at 6,809,656 to 6,809,892
_5993
Pf6N2E2_5994 - Superfamily II DNA/RNA helicases, SNF2 family, at 6,810,094 to 6,810,435
_5994
Position (kb)
6808
6809
6810 Strain fitness (log2 ratio)
-1
0
1
2 at 6807.927 kb on + strand at 6807.967 kb on + strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.968 kb on - strand at 6807.971 kb on + strand at 6807.971 kb on + strand at 6807.972 kb on - strand at 6808.040 kb on - strand, within Pf6N2E2_5990 at 6808.040 kb on - strand, within Pf6N2E2_5990 at 6808.446 kb on + strand at 6808.446 kb on + strand at 6808.446 kb on + strand at 6808.446 kb on + strand at 6808.447 kb on - strand at 6808.526 kb on + strand at 6808.526 kb on + strand at 6808.540 kb on + strand at 6808.541 kb on - strand at 6808.562 kb on + strand at 6808.563 kb on - strand at 6808.563 kb on - strand at 6808.566 kb on - strand at 6808.572 kb on + strand at 6808.573 kb on - strand at 6808.573 kb on - strand at 6808.573 kb on - strand at 6808.573 kb on - strand at 6808.653 kb on + strand, within Pf6N2E2_5991 at 6808.653 kb on + strand, within Pf6N2E2_5991 at 6808.654 kb on - strand, within Pf6N2E2_5991 at 6808.654 kb on - strand, within Pf6N2E2_5991 at 6808.849 kb on + strand at 6808.849 kb on + strand at 6808.850 kb on - strand at 6808.871 kb on + strand at 6808.871 kb on + strand at 6808.871 kb on + strand at 6808.872 kb on - strand at 6808.892 kb on + strand at 6808.893 kb on - strand at 6808.905 kb on + strand at 6808.905 kb on + strand at 6808.905 kb on + strand at 6808.905 kb on + strand at 6808.905 kb on + strand at 6808.923 kb on + strand at 6808.923 kb on + strand at 6808.924 kb on - strand at 6808.924 kb on - strand at 6808.924 kb on - strand at 6809.011 kb on - strand, within Pf6N2E2_5992 at 6809.047 kb on + strand, within Pf6N2E2_5992 at 6809.048 kb on - strand, within Pf6N2E2_5992 at 6809.048 kb on - strand, within Pf6N2E2_5992 at 6809.048 kb on - strand, within Pf6N2E2_5992 at 6809.048 kb on - strand, within Pf6N2E2_5992 at 6809.048 kb on - strand, within Pf6N2E2_5992 at 6809.194 kb on + strand, within Pf6N2E2_5992 at 6809.194 kb on + strand, within Pf6N2E2_5992 at 6809.194 kb on + strand, within Pf6N2E2_5992 at 6809.402 kb on - strand, within Pf6N2E2_5992 at 6809.432 kb on + strand, within Pf6N2E2_5992 at 6809.432 kb on + strand, within Pf6N2E2_5992 at 6809.433 kb on - strand at 6809.433 kb on - strand, within Pf6N2E2_5992 at 6809.616 kb on + strand at 6809.617 kb on - strand at 6809.617 kb on - strand at 6809.617 kb on - strand at 6809.617 kb on - strand at 6809.733 kb on + strand, within Pf6N2E2_5993 at 6809.734 kb on - strand, within Pf6N2E2_5993 at 6809.877 kb on + strand at 6809.877 kb on + strand at 6809.877 kb on + strand at 6809.877 kb on + strand at 6809.878 kb on - strand at 6809.878 kb on - strand at 6809.987 kb on + strand at 6809.987 kb on + strand at 6809.987 kb on + strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6809.988 kb on - strand at 6810.021 kb on + strand at 6810.106 kb on + strand at 6810.107 kb on - strand at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.316 kb on + strand, within Pf6N2E2_5994 at 6810.317 kb on - strand, within Pf6N2E2_5994 at 6810.325 kb on + strand, within Pf6N2E2_5994 at 6810.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,807,927 + -0.1 6,807,967 + +0.6 6,807,968 - -0.3 6,807,968 - +0.0 6,807,968 - -0.5 6,807,968 - +0.1 6,807,968 - -0.1 6,807,968 - +0.9 6,807,968 - +1.4 6,807,971 + +1.1 6,807,971 + +0.2 6,807,972 - -0.3 6,808,040 - Pf6N2E2_5990 0.14 +0.7 6,808,040 - Pf6N2E2_5990 0.14 +0.2 6,808,446 + +0.4 6,808,446 + +1.1 6,808,446 + -1.0 6,808,446 + +0.1 6,808,447 - +0.5 6,808,526 + -1.0 6,808,526 + -0.5 6,808,540 + +1.0 6,808,541 - +0.5 6,808,562 + -0.1 6,808,563 - +0.2 6,808,563 - +0.8 6,808,566 - +0.0 6,808,572 + +0.6 6,808,573 - -0.5 6,808,573 - +0.7 6,808,573 - -0.9 6,808,573 - -0.1 6,808,653 + Pf6N2E2_5991 0.22 -0.1 6,808,653 + Pf6N2E2_5991 0.22 -1.1 6,808,654 - Pf6N2E2_5991 0.22 +0.9 6,808,654 - Pf6N2E2_5991 0.22 +1.0 6,808,849 + +1.5 6,808,849 + +2.5 6,808,850 - +0.5 6,808,871 + -0.1 6,808,871 + +0.4 6,808,871 + +0.9 6,808,872 - -0.7 6,808,892 + +1.3 6,808,893 - +0.2 6,808,905 + +0.4 6,808,905 + +0.3 6,808,905 + +1.5 6,808,905 + +0.6 6,808,905 + +0.3 6,808,923 + +1.0 6,808,923 + +0.7 6,808,924 - +0.6 6,808,924 - -0.3 6,808,924 - -0.6 6,809,011 - Pf6N2E2_5992 0.17 +0.0 6,809,047 + Pf6N2E2_5992 0.23 +0.2 6,809,048 - Pf6N2E2_5992 0.23 +0.1 6,809,048 - Pf6N2E2_5992 0.23 +1.0 6,809,048 - Pf6N2E2_5992 0.23 +1.4 6,809,048 - Pf6N2E2_5992 0.23 +0.9 6,809,048 - Pf6N2E2_5992 0.23 +1.6 6,809,194 + Pf6N2E2_5992 0.47 +1.6 6,809,194 + Pf6N2E2_5992 0.47 +1.9 6,809,194 + Pf6N2E2_5992 0.47 +1.0 6,809,402 - Pf6N2E2_5992 0.81 +0.7 6,809,432 + Pf6N2E2_5992 0.86 +0.2 6,809,432 + Pf6N2E2_5992 0.86 +0.1 6,809,433 - -1.7 6,809,433 - Pf6N2E2_5992 0.87 +0.1 6,809,616 + +0.6 6,809,617 - -0.6 6,809,617 - +0.3 6,809,617 - +0.3 6,809,617 - -1.1 6,809,733 + Pf6N2E2_5993 0.32 -0.3 6,809,734 - Pf6N2E2_5993 0.33 +0.6 6,809,877 + +0.1 6,809,877 + -1.1 6,809,877 + -0.4 6,809,877 + +0.2 6,809,878 - -0.1 6,809,878 - +0.9 6,809,987 + -0.2 6,809,987 + -0.6 6,809,987 + -0.4 6,809,988 - +0.4 6,809,988 - -0.4 6,809,988 - +1.9 6,809,988 - +0.4 6,809,988 - -0.9 6,809,988 - +0.1 6,809,988 - +0.9 6,810,021 + +1.4 6,810,106 + +0.5 6,810,107 - +0.6 6,810,316 + Pf6N2E2_5994 0.65 -0.5 6,810,316 + Pf6N2E2_5994 0.65 -1.1 6,810,316 + Pf6N2E2_5994 0.65 -0.8 6,810,316 + Pf6N2E2_5994 0.65 +1.3 6,810,316 + Pf6N2E2_5994 0.65 +0.3 6,810,316 + Pf6N2E2_5994 0.65 +0.4 6,810,316 + Pf6N2E2_5994 0.65 -0.1 6,810,317 - Pf6N2E2_5994 0.65 -0.1 6,810,325 + Pf6N2E2_5994 0.68 +0.2 6,810,501 - +1.7
Or see this region's nucleotide sequence